Detailed information NTG0334547

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: EPY81_RS02550
• Genome accession: NZ_CP035109
• Coordinates: 512176..513234 (-)
• Length: 352 a.a.
• NCBI ID: WP_031945632.1
• Uniprot ID: A0A8I1HC74
• Organism: Acinetobacter pittii strain NQ-003
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 507176..518234

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EPY81_RS02525 (EPY81_02535)	aroK	507439..508008 (-)	570	WP_002116987.1	shikimate kinase AroK	-
EPY81_RS02530 (EPY81_02540)	pilQ	508020..510185 (-)	2166	WP_002116833.1	type IV pilus secretin PilQ	Machinery gene
EPY81_RS02535 (EPY81_02545)	pilP	510248..510775 (-)	528	WP_002116864.1	pilus assembly protein PilP	Machinery gene
EPY81_RS02540 (EPY81_02550)	pilO	510786..511538 (-)	753	WP_002116684.1	type 4a pilus biogenesis protein PilO	Machinery gene
EPY81_RS02545 (EPY81_02555)	pilN	511535..512176 (-)	642	WP_002116944.1	PilN domain-containing protein	Machinery gene
EPY81_RS02550 (EPY81_02560)	pilM	512176..513234 (-)	1059	WP_031945632.1	pilus assembly protein PilM	Machinery gene
EPY81_RS02555 (EPY81_02565)	ponA	513395..515956 (+)	2562	WP_002116655.1	penicillin-binding protein PBP1a	-
EPY81_RS02560 (EPY81_02570)	rrmA	516011..516835 (-)	825	WP_002117059.1	putative RNA methyltransferase	-
EPY81_RS02565 (EPY81_02575)	-	516838..517170 (-)	333	WP_002116981.1	DUF7709 family protein	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39054.62 Da
• Isoelectric Point: 4.4657

>NTDB_id=334547 EPY81_RS02550 WP_031945632.1 512176..513234(-) (pilM) [Acinetobacter pittii strain NQ-003]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALTPLPENSVVEKNILNPEAVAEALERAMNLANPHTTNAAI
AVPTSTVIHKTIEMDADMNDDEREVQIRLDAEQYIPFPLDEVSLDFEVLPDRLPNPNRVNVLLVATRTENVETRVEVLEL
VDLTPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLSLEIQSRYGLSLEE
AGRAKKERSLPDDYDIEILEPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=334547 EPY81_RS02550 WP_031945632.1 512176..513234(-) (pilM) [Acinetobacter pittii strain NQ-003]
GTGCTCAGGTTATATCGTAAACCGAATAAGGGGTTAATGGGCGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTTAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTAACGCCTTTACCTGAGAACAGTGTAGTTGAAAAAAATA
TTTTGAATCCAGAAGCAGTTGCAGAAGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCACACCACTAACGCTGCAATT
GCTGTTCCAACATCGACGGTTATTCATAAAACCATCGAAATGGATGCAGATATGAACGATGATGAACGTGAAGTTCAGAT
TCGATTAGATGCGGAGCAATATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTACCAGATCGACTGC
CGAATCCAAATCGTGTGAATGTACTTTTAGTCGCAACACGAACAGAAAATGTTGAAACACGCGTAGAAGTACTGGAACTA
GTTGATTTAACGCCTAAGCTTGCTGATGTTGAAAGTTATGCTGTTGAGCGTGCTTTTAGTGTGTTTGCTGATAGCTTACC
CATGGGTGCAAATACCATTGGTATTTTAGATATTGGCCATACCATGACGACATTATCGGTTATGCAAAATGGCAAAATCA
TTTATACCCGTGAACAAGTCTTTGGCGGTAAACAGCTTAGTTTGGAAATTCAAAGCCGTTACGGTTTGTCATTAGAAGAA
GCGGGACGCGCTAAAAAAGAACGTTCTTTGCCAGATGATTATGACATCGAAATCTTAGAACCATTTTTAGATGCTGTAGT
TCAGCAAGCTGCTAGATCTCTACAATTTTTCTTCTCTTCATCCCAATTCAATGAAATTGACCATATTTTGCTAGCGGGTG
GCAATGCCAACATTCCAGGGCTTGCTAAATTATTGCAACAAAAACTCGGTTACCGAGTCACTATTGCCAATCCATTTTTA
CAAATGGGTTTCTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCTTCATTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	96.023	100	0.96
comM	Acinetobacter nosocomialis M2	96.023	100	0.96
comM	Acinetobacter baylyi ADP1	80.966	100	0.81
pilM	Legionella pneumophila strain ERS1305867	41.808	100	0.42