Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   EPY81_RS02540 Genome accession   NZ_CP035109
Coordinates   510786..511538 (-) Length   250 a.a.
NCBI ID   WP_002116684.1    Uniprot ID   -
Organism   Acinetobacter pittii strain NQ-003     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 505786..516538
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EPY81_RS02520 (EPY81_02530) aroB 506328..507410 (-) 1083 WP_002116776.1 3-dehydroquinate synthase -
  EPY81_RS02525 (EPY81_02535) aroK 507439..508008 (-) 570 WP_002116987.1 shikimate kinase AroK -
  EPY81_RS02530 (EPY81_02540) pilQ 508020..510185 (-) 2166 WP_002116833.1 type IV pilus secretin PilQ Machinery gene
  EPY81_RS02535 (EPY81_02545) pilP 510248..510775 (-) 528 WP_002116864.1 pilus assembly protein PilP Machinery gene
  EPY81_RS02540 (EPY81_02550) pilO 510786..511538 (-) 753 WP_002116684.1 type 4a pilus biogenesis protein PilO Machinery gene
  EPY81_RS02545 (EPY81_02555) pilN 511535..512176 (-) 642 WP_002116944.1 PilN domain-containing protein Machinery gene
  EPY81_RS02550 (EPY81_02560) pilM 512176..513234 (-) 1059 WP_031945632.1 pilus assembly protein PilM Machinery gene
  EPY81_RS02555 (EPY81_02565) ponA 513395..515956 (+) 2562 WP_002116655.1 penicillin-binding protein PBP1a -

Sequence

Protein


Download         Length: 250 a.a.        Molecular weight: 28201.04 Da        Isoelectric Point: 4.8782

>NTDB_id=334545 EPY81_RS02540 WP_002116684.1 510786..511538(-) (pilO) [Acinetobacter pittii strain NQ-003]
MSPDELHELSLEQAASKKKKITLEKFLQQFNTLDMNSYGSWPLSVKITCWIFIFFAVLALGYFVAIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQLQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
ITIEATGDYHAFGAFVSSIAALPRIVTMHDFVVEAAPVKDGKSDIPVLNYSIKAKTYRYMGAVENPEHQEKNASASSVAP
DSSTANTQPK

Nucleotide


Download         Length: 753 bp        

>NTDB_id=334545 EPY81_RS02540 WP_002116684.1 510786..511538(-) (pilO) [Acinetobacter pittii strain NQ-003]
ATGAGTCCTGATGAACTTCATGAGCTGTCTTTAGAGCAAGCTGCATCTAAGAAAAAAAAGATCACCTTGGAAAAATTTCT
TCAGCAATTTAACACGCTCGATATGAACAGTTATGGCAGCTGGCCATTATCTGTGAAAATTACGTGTTGGATTTTTATTT
TCTTTGCCGTATTGGCTTTGGGGTATTTTGTTGCTATTCAGCCAAAGCTACAAGCAATTGATAATGCTCAAGCACAAGAA
AGTAATTTATTAAATGAGTTTCGAGAAAAAGACTCAAAATTACGCAACTTGCAGCAATACCAGCTTCAGCTTCAAGAGAT
GCAAGCCAACTTCAATCAGCAGTTAGAACAATTGCCAAAAGAAACCGAAATTCCAAGTTTAGTTGAAGATATTAACTTAA
CGGGGGTGAATTCTGGTCTGAAGTTTAAAAATATCCGCTTAGAAGATGAAGTAAAGCAGGAAATTTTTATTGAACAGCCT
ATTACTATCGAAGCAACTGGGGATTATCACGCTTTCGGTGCTTTTGTTAGCAGTATTGCGGCATTACCACGTATTGTGAC
AATGCATGATTTTGTTGTTGAAGCTGCTCCTGTTAAGGATGGTAAATCAGATATTCCTGTGCTTAATTATTCTATTAAAG
CAAAAACTTATCGCTATATGGGTGCTGTAGAAAATCCTGAACATCAGGAAAAAAATGCTTCGGCTTCCTCGGTAGCACCT
GATTCTTCTACTGCTAACACACAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

83.6

100

0.836
  comO Acinetobacter baylyi ADP1

70

96

0.672

Multiple sequence alignment