Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ELR70_RS22285 Genome accession   NZ_CP034835
Coordinates   3916957..3918633 (+) Length   558 a.a.
NCBI ID   WP_054014753.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. R3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3911957..3923633
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ELR70_RS22260 (ELR70_22265) nadC 3912355..3913194 (+) 840 WP_054014748.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ELR70_RS25780 (ELR70_22270) - 3913595..3914050 (+) 456 WP_054014749.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ELR70_RS22270 (ELR70_22275) - 3914107..3915338 (+) 1232 Protein_3396 type II secretion system F family protein -
  ELR70_RS22275 (ELR70_22280) pilD 3915358..3916233 (+) 876 WP_054014751.1 A24 family peptidase Machinery gene
  ELR70_RS22280 (ELR70_22285) coaE 3916238..3916849 (+) 612 WP_054014752.1 dephospho-CoA kinase -
  ELR70_RS22285 (ELR70_22290) pilB 3916957..3918633 (+) 1677 WP_054014753.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ELR70_RS22290 (ELR70_22295) yacG 3918705..3918947 (+) 243 WP_054014754.1 DNA gyrase inhibitor YacG -
  ELR70_RS22295 (ELR70_22300) - 3919006..3919515 (-) 510 WP_054014755.1 Spy/CpxP family protein refolding chaperone -
  ELR70_RS22300 (ELR70_22305) - 3919724..3920410 (+) 687 WP_054014756.1 response regulator transcription factor -
  ELR70_RS22305 (ELR70_22310) - 3920412..3921791 (+) 1380 WP_054014757.1 ATP-binding protein -
  ELR70_RS22310 (ELR70_22315) - 3921781..3922797 (+) 1017 WP_054014758.1 alpha/beta hydrolase -
  ELR70_RS22315 (ELR70_22320) - 3922749..3923594 (-) 846 WP_082353163.1 cation diffusion facilitator family transporter -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 62252.29 Da        Isoelectric Point: 5.5480

>NTDB_id=333325 ELR70_RS22285 WP_054014753.1 3916957..3918633(+) (pilB) [Pseudoalteromonas sp. R3]
MEHHSPLLRKFITLGKLTAEQVKAKQADAHSTAELICLCSGMSGQEMAEQCLDLFRVPYFDLSGFDITQVPKELVKEKLI
RQHHLLPLVKKDRKLFIATSDPTDYGAFENFEFSTGLQCEVVVVDYKLLDQKIDQLFDDNSSLSLSEDEFKEFASLDVED
EPKTQNTDEEKDDAPIIVYINKILMDAIKKGASDLHFEPYEKKYRVRFRIDGLLHEMASPPHALSSRLSARIKVMSHLDI
AEKRKPQDGRIKLKISERKSIDFRVSTLPTMWGEKIVMRILDSSAAKLGIDALGYEADQKDMYLNALEQPQGMILVTGPT
GSGKTVSLYTGLNILNKPERNISTAEDPVEINLEGINQVQINTKADMTFANALRAFLRQDPDVVMVGEIRDLETAEIAIK
AAQTGHLVMSTLHTNSASETLTRLLNMGVPAYNVASSVSLIIAQRLARRLCPKCKVPEDLPAEELQRQGFSEEQIQNMTV
YSPKGCDHCTDGYKGRVGIYEVMKITPEISHAIMDGGNSLEIAELATKQGFANLRQSGLKKAADGITSLAEVNRVTNY

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=333325 ELR70_RS22285 WP_054014753.1 3916957..3918633(+) (pilB) [Pseudoalteromonas sp. R3]
ATGGAACATCACTCGCCATTACTGAGAAAGTTTATTACCCTTGGCAAACTGACTGCTGAGCAGGTCAAAGCCAAACAAGC
CGACGCCCACTCCACCGCTGAACTTATTTGTCTGTGCAGCGGCATGAGCGGTCAGGAAATGGCCGAGCAATGTCTGGACC
TTTTCAGAGTCCCCTACTTTGACCTAAGCGGTTTTGATATTACTCAGGTACCTAAAGAGCTGGTAAAGGAAAAACTGATC
CGCCAACACCACCTGCTGCCACTGGTTAAAAAGGACAGAAAACTCTTCATTGCCACGTCAGATCCGACTGACTACGGGGC
ATTTGAAAACTTTGAGTTCAGTACCGGTTTACAGTGTGAGGTTGTCGTCGTAGATTACAAACTGCTCGATCAGAAAATTG
ATCAGCTCTTTGACGACAATAGTAGCCTGAGCCTCAGTGAAGACGAGTTTAAAGAATTCGCCAGTCTGGATGTCGAAGAC
GAACCAAAAACACAAAACACGGACGAAGAGAAAGATGATGCGCCAATTATTGTCTACATCAATAAAATTTTGATGGATGC
GATAAAAAAAGGCGCTTCGGATCTCCACTTCGAACCTTATGAAAAAAAATACCGAGTGCGTTTTCGTATTGATGGCTTAT
TACACGAAATGGCCAGTCCGCCACACGCGCTGTCCAGCCGCTTATCAGCTCGTATCAAGGTTATGTCACACCTGGATATC
GCAGAGAAGCGCAAACCACAGGATGGCCGCATTAAACTGAAAATCTCGGAGCGCAAGAGTATTGATTTTCGTGTCAGTAC
ACTGCCTACCATGTGGGGCGAAAAGATTGTAATGCGGATCCTGGATTCATCCGCCGCGAAACTGGGGATCGATGCTCTAG
GCTATGAGGCTGACCAAAAAGACATGTACCTCAATGCCCTAGAGCAACCACAAGGGATGATACTGGTGACCGGCCCAACC
GGTTCGGGTAAAACGGTTTCTTTGTATACTGGTCTGAATATACTCAATAAACCAGAGCGAAATATCAGCACCGCGGAAGA
CCCAGTGGAAATCAACCTCGAAGGGATCAATCAGGTTCAAATTAACACCAAAGCCGATATGACCTTTGCAAATGCCCTGC
GGGCATTCCTGCGTCAGGATCCCGATGTTGTCATGGTTGGTGAGATCCGGGATCTGGAAACCGCCGAGATTGCCATCAAA
GCTGCACAAACAGGTCACCTGGTGATGAGTACCCTGCACACCAATTCAGCTTCAGAAACCCTGACCCGACTGCTCAACAT
GGGTGTCCCCGCCTATAACGTGGCAAGTTCAGTCAGTCTTATCATTGCACAGCGCCTGGCCCGGCGTCTCTGCCCCAAGT
GTAAAGTGCCAGAAGATCTGCCAGCAGAAGAATTGCAACGTCAGGGCTTTAGCGAGGAGCAGATACAAAACATGACAGTC
TATTCACCCAAAGGATGCGATCACTGTACTGATGGCTATAAAGGGCGTGTGGGCATTTATGAAGTGATGAAAATCACACC
GGAGATCTCTCACGCTATCATGGATGGCGGTAATTCTCTGGAAATCGCTGAACTGGCTACCAAACAGGGCTTTGCGAATC
TGAGACAATCTGGACTGAAAAAAGCAGCTGATGGGATCACTTCACTGGCGGAAGTAAACCGGGTTACCAACTACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-141)

Type II/IV secretion system protein (T2SSE)

(182-449)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.63

96.774

0.577
  pilB Acinetobacter baylyi ADP1

58.743

91.219

0.536
  pilB Vibrio cholerae strain A1552

53.734

98.387

0.529
  pilB Vibrio parahaemolyticus RIMD 2210633

54.144

97.312

0.527
  pilB Legionella pneumophila strain ERS1305867

55.662

93.369

0.52
  pilB Vibrio campbellii strain DS40M4

53.125

97.491

0.518
  pilF Neisseria gonorrhoeae MS11

47.491

100

0.475
  pilB/pilB1 Synechocystis sp. PCC 6803

36.542

100

0.401

Multiple sequence alignment