Detailed information NTG0333324

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: ELR70_RS22275
• Genome accession: NZ_CP034835
• Coordinates: 3915358..3916233 (+)
• Length: 291 a.a.
• NCBI ID: WP_054014751.1
• Uniprot ID: A0A3S5I628
• Organism: Pseudoalteromonas sp. R3
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3910358..3921233

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ELR70_RS22250 (ELR70_22255)	ampE	3910733..3911617 (-)	885	WP_054014746.1	beta-lactamase regulator AmpE	-
ELR70_RS22255 (ELR70_22260)	ampD	3911623..3912165 (-)	543	WP_054014747.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
ELR70_RS22260 (ELR70_22265)	nadC	3912355..3913194 (+)	840	WP_054014748.1	carboxylating nicotinate-nucleotide diphosphorylase	-
ELR70_RS25780 (ELR70_22270)	-	3913595..3914050 (+)	456	WP_054014749.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
ELR70_RS22270 (ELR70_22275)	-	3914107..3915338 (+)	1232	Protein_3396	type II secretion system F family protein	-
ELR70_RS22275 (ELR70_22280)	pilD	3915358..3916233 (+)	876	WP_054014751.1	A24 family peptidase	Machinery gene
ELR70_RS22280 (ELR70_22285)	coaE	3916238..3916849 (+)	612	WP_054014752.1	dephospho-CoA kinase	-
ELR70_RS22285 (ELR70_22290)	pilB	3916957..3918633 (+)	1677	WP_054014753.1	type IV-A pilus assembly ATPase PilB	Machinery gene
ELR70_RS22290 (ELR70_22295)	yacG	3918705..3918947 (+)	243	WP_054014754.1	DNA gyrase inhibitor YacG	-
ELR70_RS22295 (ELR70_22300)	-	3919006..3919515 (-)	510	WP_054014755.1	Spy/CpxP family protein refolding chaperone	-
ELR70_RS22300 (ELR70_22305)	-	3919724..3920410 (+)	687	WP_054014756.1	response regulator transcription factor	-

Sequence

Protein

• Length: 291 a.a.
• Molecular weight: 32422.72 Da
• Isoelectric Point: 7.9704

>NTDB_id=333324 ELR70_RS22275 WP_054014751.1 3915358..3916233(+) (pilD) [Pseudoalteromonas sp. R3]
MQEVWHLMEQQTWFWLLTVALTSLCIGSFLNVVIYRLPIMMQRGWQAECRVILSQEPEHPEPAPFNLMTPGSTCPKCNSK
IKVHHNIPLLSWLFLKGKCAYCSTPIAKRYPLIELLTGVASVWLAWHFSATPQAVVYLLVTWVLIALIFIDIDHMLLPDQ
LTLPLLWFALLAAVFGITITPSQAIIGAAVGYLSLWSIYWAFKLLTGKEGMGFGDFKLLAVFGALMGWQSLLLIVLLSSV
VGAVIGATQLAVQGKDKATPIPFGPYLAIAGWLTLLYGEQISRWYFSLLGV

Nucleotide

• Length: 876 bp

>NTDB_id=333324 ELR70_RS22275 WP_054014751.1 3915358..3916233(+) (pilD) [Pseudoalteromonas sp. R3]
ATGCAAGAAGTTTGGCATCTCATGGAGCAGCAAACCTGGTTTTGGTTGCTGACCGTCGCTCTGACTAGCCTATGTATCGG
CAGCTTTTTAAACGTCGTAATCTATCGTTTACCTATCATGATGCAACGCGGTTGGCAGGCAGAATGCAGGGTGATTTTAT
CTCAGGAGCCTGAACATCCCGAACCCGCTCCATTTAACCTGATGACGCCAGGCTCAACCTGTCCGAAGTGTAACAGTAAA
ATTAAAGTTCATCACAACATTCCCCTGCTCAGCTGGTTATTTTTGAAAGGTAAATGCGCATACTGCAGCACGCCTATTGC
CAAACGTTACCCACTTATCGAATTACTCACTGGAGTTGCCTCCGTGTGGCTGGCCTGGCATTTTTCTGCAACTCCACAGG
CAGTTGTTTACTTACTTGTGACCTGGGTACTCATCGCACTGATTTTTATCGACATTGACCATATGTTACTACCCGATCAG
CTGACCTTGCCCTTACTCTGGTTCGCACTGTTGGCCGCCGTATTTGGTATAACTATTACGCCTTCTCAAGCGATCATTGG
CGCAGCTGTTGGCTATTTGAGCCTGTGGAGTATTTATTGGGCTTTTAAGCTGCTGACAGGAAAAGAAGGCATGGGATTTG
GTGATTTTAAACTGCTGGCGGTATTTGGTGCACTTATGGGCTGGCAGTCATTATTGCTGATTGTATTGTTGTCTAGTGTC
GTAGGTGCCGTGATCGGTGCTACTCAGCTTGCCGTGCAAGGGAAAGACAAAGCCACTCCTATCCCATTTGGCCCTTACCT
CGCTATTGCTGGCTGGCTAACCTTGTTATATGGTGAGCAAATCAGTCGCTGGTACTTTTCGCTATTAGGAGTTTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (139-246)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (22-128)

3D structure

• AlphaFold DB: A0A3S5I628

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	55.435	94.845	0.526
pilD	Vibrio campbellii strain DS40M4	51.042	98.969	0.505
pilD	Acinetobacter nosocomialis M2	49.477	98.625	0.488
pilD	Acinetobacter baumannii D1279779	49.129	98.625	0.485
pilD	Neisseria gonorrhoeae MS11	43.939	90.722	0.399