Detailed information NTG0033262

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: SPP_RS10275
• Genome accession: NC_012467
• Coordinates: 1907901..1908362 (-)
• Length: 153 a.a.
• NCBI ID: WP_000250531.1
• Uniprot ID: -
• Organism: Streptococcus pneumoniae P1031
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1902901..1913362

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SPP_RS10240 (SPP_2077)	-	1903205..1904029 (-)	825	WP_000727904.1	membrane protein insertase YidC	-
SPP_RS10245 (SPP_2078)	rnpA	1904004..1904375 (-)	372	WP_000739246.1	ribonuclease P protein component	-
SPP_RS13780 (SPP_2079)	-	1904392..1904523 (-)	132	WP_000768904.1	hypothetical protein	-
SPP_RS10250 (SPP_2080)	-	1904524..1905714 (-)	1191	WP_000167766.1	acetate kinase	-
SPP_RS10255 (SPP_2081)	comYH	1905765..1906718 (-)	954	WP_000345116.1	class I SAM-dependent methyltransferase	Machinery gene
SPP_RS12415	-	1906779..1907373 (-)	595	Protein_1944	class I SAM-dependent methyltransferase	-
SPP_RS10270 (SPP_2085)	comGG/cglG	1907510..1907923 (-)	414	WP_000265631.1	competence type IV pilus minor pilin ComGG	Machinery gene
SPP_RS10275 (SPP_2086)	comGF/cglF	1907901..1908362 (-)	462	WP_000250531.1	competence type IV pilus minor pilin ComGF	Machinery gene
SPP_RS10280 (SPP_2087)	comGE/cglE	1908325..1908627 (-)	303	WP_000413382.1	competence type IV pilus minor pilin ComGE	Machinery gene
SPP_RS10285 (SPP_2088)	comGD/cglD	1908590..1908994 (-)	405	WP_000588020.1	competence type IV pilus minor pilin ComGD	Machinery gene
SPP_RS10290 (SPP_2089)	comGC/cglC	1908987..1909313 (-)	327	WP_000738633.1	comG operon protein ComGC	Machinery gene
SPP_RS10295 (SPP_2090)	comGB/cglB	1909315..1910331 (-)	1017	WP_001844679.1	competence type IV pilus assembly protein ComGB	Machinery gene
SPP_RS10300 (SPP_2091)	comGA/cglA/cilD	1910279..1911220 (-)	942	WP_000249571.1	competence type IV pilus ATPase ComGA	Machinery gene
SPP_RS10305 (SPP_2092)	-	1911296..1911661 (-)	366	WP_000286415.1	DUF1033 family protein	-
SPP_RS10310 (SPP_2093)	-	1911812..1912870 (-)	1059	WP_000649471.1	zinc-dependent alcohol dehydrogenase family protein	-

Sequence

Protein

• Length: 153 a.a.
• Molecular weight: 17849.37 Da
• Isoelectric Point: 6.4832

>NTDB_id=33262 SPP_RS10275 WP_000250531.1 1907901..1908362(-) (comGF/cglF) [Streptococcus pneumoniae P1031]
MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLISEVRYQQQSEQKEWLLFVDQLEVELDRSQFEKVEGN
RLYMKQDGKDIAIGKSKSDDFRKTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES

Nucleotide

• Length: 462 bp

>NTDB_id=33262 SPP_RS10275 WP_000250531.1 1907901..1908362(-) (comGF/cglF) [Streptococcus pneumoniae P1031]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTGCTTGCCCTCAT
TGTCATCAGTGGGGGATTACTCCTTTTTCAAGCTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGACCAACTTGAGGTAGAATTAGACCGTTCGCAGTTCGAAAAAGTAGAAGGCAAT
CGCCTATACATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGACGATTTTCGTAAAACCGATGCTAG
TGGACGGGGGTATCAGCCTATGGTTTATGGCCTCAAATCAGCTCAGATTACAGAGGACAATCAACTGGTTCATTTTCGTT
TCCAGTTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAGAAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (45-141)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus pneumoniae Rx1	95.425	100	0.954
comGF/cglF	Streptococcus pneumoniae D39	95.425	100	0.954
comGF/cglF	Streptococcus pneumoniae R6	95.425	100	0.954
comGF/cglF	Streptococcus pneumoniae TIGR4	95.425	100	0.954
comGF/cglF	Streptococcus mitis SK321	92.81	100	0.928
comGF/cglF	Streptococcus mitis NCTC 12261	91.503	100	0.915