Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ELB75_RS12220 Genome accession   NZ_CP034670
Coordinates   2417020..2418684 (+) Length   554 a.a.
NCBI ID   WP_126984126.1    Uniprot ID   A0A3S9SMF8
Organism   Eikenella corrodens strain KCOM 3110     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2412020..2423684
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ELB75_RS12195 (ELB75_12195) - 2412137..2412691 (+) 555 WP_126984124.1 hypothetical protein -
  ELB75_RS12200 (ELB75_12200) fumC 2413045..2414439 (-) 1395 WP_126984306.1 class II fumarate hydratase -
  ELB75_RS12215 (ELB75_12215) recJ 2415077..2416774 (+) 1698 WP_126984125.1 single-stranded-DNA-specific exonuclease RecJ -
  ELB75_RS12220 (ELB75_12220) pilF 2417020..2418684 (+) 1665 WP_126984126.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ELB75_RS12225 (ELB75_12225) pilG 2418716..2419951 (+) 1236 WP_126984127.1 type II secretion system F family protein Machinery gene
  ELB75_RS12230 (ELB75_12230) pilD 2420043..2420900 (+) 858 WP_126984128.1 A24 family peptidase Machinery gene
  ELB75_RS12235 (ELB75_12235) coaE 2420902..2421507 (+) 606 WP_126984129.1 dephospho-CoA kinase -
  ELB75_RS12240 (ELB75_12240) zapD 2421511..2422287 (+) 777 WP_206501462.1 cell division protein ZapD -
  ELB75_RS12245 (ELB75_12245) yacG 2422284..2422466 (+) 183 WP_126984130.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 554 a.a.        Molecular weight: 61868.59 Da        Isoelectric Point: 5.7684

>NTDB_id=332344 ELB75_RS12220 WP_126984126.1 2417020..2418684(+) (pilF) [Eikenella corrodens strain KCOM 3110]
MSVGLLRVLFQNKLIGQDQVEKYRENINQNKNIIPMLAEAGIIQQKDLLLLLAKLFHYPMMDLSRYSRSMLVQDVINDTQ
MLEYRCVPLFKRGQKLFLGVSNPTLLQIYQQLVFPSGLSVDLVLVNDEQLDRLLEFASQASTAILDEISENPDGKPILLD
GESEDGPIAKFIHKVLSDALNTGASDIHFEFYEEMARIRFRTDGQLREVVQPPLNIRGQIASRIKVMSKMDISEKRIPQD
GRIQIQFHKNQKPIDFRVNTLPTLFGEKIVMRILNSDAATLNIDQLGFEPFQKELLLEAIHRPYGMVLVTGPTGSGKTVS
LYTCLNILNTDSVNISTAEDPAEINLPGINQVNVNDKQGMTFAAALKAFLRQDPDIIMVGEIRDLETADIAIKAAQTGHM
VFSTLHTNNAPATLSRMLNMGVAPFNIASSVNLIMAQRLVRRLCTCKQPIERPPAEALLKVGFTEEDLAKDAWQLYRQVG
CDRCRGKGFKGRAGVYEIMPVTDAMQKIIINNGTEVDIAQQAYADGLVDLRRAGILKAMQGITTLEEILASTND

Nucleotide


Download         Length: 1665 bp        

>NTDB_id=332344 ELB75_RS12220 WP_126984126.1 2417020..2418684(+) (pilF) [Eikenella corrodens strain KCOM 3110]
ATGAGCGTCGGACTATTGCGTGTCCTGTTTCAAAATAAGTTGATTGGGCAGGATCAGGTTGAAAAATACCGAGAAAACAT
TAATCAAAACAAAAATATCATTCCTATGCTAGCTGAGGCAGGCATTATCCAACAAAAAGATTTACTGTTGCTGCTGGCTA
AATTGTTCCATTATCCGATGATGGATTTATCCCGCTACTCGCGTTCGATGCTGGTACAGGATGTCATTAATGATACCCAG
ATGCTGGAGTACCGTTGTGTCCCTTTATTCAAGCGTGGGCAAAAACTGTTTTTAGGTGTATCCAACCCCACACTGTTGCA
AATATACCAACAATTGGTTTTTCCCAGTGGTTTGTCCGTCGATTTGGTGCTGGTCAATGACGAGCAGCTGGATAGATTGC
TGGAATTCGCCAGCCAAGCCTCCACTGCCATCCTAGATGAAATTTCCGAGAATCCGGATGGCAAACCAATATTACTGGAC
GGCGAATCAGAAGACGGCCCGATTGCCAAATTCATTCACAAAGTGCTGTCTGATGCCCTAAATACCGGCGCCTCAGATAT
CCATTTTGAGTTTTATGAAGAAATGGCACGCATCCGATTCCGTACTGACGGTCAATTGCGCGAAGTGGTGCAGCCTCCGC
TCAACATCCGCGGGCAGATTGCTTCGCGTATCAAGGTAATGTCGAAAATGGACATTTCCGAAAAACGCATACCGCAGGAC
GGCCGTATCCAGATCCAGTTCCATAAAAACCAAAAGCCGATCGACTTTCGTGTAAATACGCTGCCTACACTATTCGGCGA
AAAAATTGTAATGCGTATTCTGAATTCAGATGCTGCCACGTTGAATATCGACCAACTGGGTTTCGAACCTTTCCAAAAAG
AATTACTACTGGAGGCCATCCACCGCCCATACGGCATGGTGCTGGTTACCGGCCCGACCGGTTCGGGTAAAACCGTGTCG
CTGTATACCTGTCTAAACATCTTGAATACCGACAGTGTAAACATCTCCACCGCCGAAGACCCGGCCGAGATTAACTTGCC
CGGTATCAATCAGGTAAACGTAAACGATAAACAGGGCATGACATTTGCTGCAGCATTGAAAGCATTCCTGCGTCAAGACC
CGGACATCATCATGGTGGGTGAGATTCGCGACTTGGAAACTGCCGATATTGCCATTAAAGCCGCGCAAACCGGCCACATG
GTGTTCTCTACCCTGCATACCAATAATGCTCCGGCCACACTATCCCGTATGTTGAACATGGGGGTAGCACCGTTCAATAT
TGCTAGCTCGGTTAATCTGATTATGGCGCAGCGTTTGGTGCGGCGGCTGTGTACCTGCAAACAACCGATCGAGCGTCCGC
CAGCTGAAGCTTTACTGAAGGTCGGCTTCACTGAAGAAGACTTAGCCAAGGACGCCTGGCAGTTATACCGTCAAGTGGGT
TGTGACCGGTGCAGAGGAAAAGGTTTCAAAGGACGAGCCGGTGTATATGAAATCATGCCGGTTACCGATGCCATGCAGAA
AATCATCATCAATAACGGTACCGAAGTGGATATTGCTCAACAAGCCTACGCTGATGGATTGGTGGACCTGCGCCGAGCCG
GTATTTTAAAAGCAATGCAAGGTATTACCACCTTAGAAGAAATCCTTGCCAGCACTAACGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-135)

Type II/IV secretion system protein (T2SSE)

(174-442)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3S9SMF8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

69.767

100

0.704
  pilB Acinetobacter baylyi ADP1

50.352

100

0.516
  pilB Acinetobacter baumannii D1279779

55.268

90.794

0.502
  pilB Vibrio cholerae strain A1552

46.004

100

0.468
  pilB Legionella pneumophila strain ERS1305867

45.633

100

0.462
  pilB Vibrio campbellii strain DS40M4

43.915

100

0.449
  pilB Vibrio parahaemolyticus RIMD 2210633

43.717

100

0.446
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.464

94.224

0.372

Multiple sequence alignment