Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KWO_RS16575 Genome accession   NZ_CP034655
Coordinates   3575395..3577128 (-) Length   577 a.a.
NCBI ID   WP_010375213.1    Uniprot ID   -
Organism   Xanthomonas vasicola pv. musacearum NCPPB 4379     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3570395..3582128
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KWO_RS16565 (KWO_016565) - 3571592..3573205 (+) 1614 WP_010375222.1 HAMP domain-containing sensor histidine kinase -
  KWO_RS16570 (KWO_016570) pilR 3573470..3574924 (+) 1455 WP_026113341.1 sigma-54 dependent transcriptional regulator Regulator
  KWO_RS16575 (KWO_016575) pilB 3575395..3577128 (-) 1734 WP_010375213.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KWO_RS16580 (KWO_016580) - 3577193..3577625 (-) 433 Protein_3051 pilin -
  KWO_RS16585 (KWO_016585) pilA/pilAI 3577701..3578117 (-) 417 WP_010375211.1 pilin Machinery gene
  KWO_RS16590 (KWO_016590) pilC 3578481..3579737 (+) 1257 WP_026113135.1 type II secretion system F family protein Machinery gene
  KWO_RS16595 (KWO_016595) - 3579744..3580607 (+) 864 WP_010375207.1 A24 family peptidase -
  KWO_RS16600 (KWO_016600) coaE 3580621..3581265 (+) 645 WP_010375205.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62803.91 Da        Isoelectric Point: 5.6039

>NTDB_id=332151 KWO_RS16575 WP_010375213.1 3575395..3577128(-) (pilB) [Xanthomonas vasicola pv. musacearum NCPPB 4379]
MNVSASANLVGITGIARRLVQDGALEESVARSAMDQAASAKIPLPQWFSEKKLVSAAQLAAANAVEFGMPLIDVSVFDAS
QNAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEAILVDEDQIRRTLEQWQASNASLGSSLG
NDDDEMGDLDVSTGDEDMGAGGDSGVDARGDDTPVVKFVNKMLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
DNALLAEGFTPEQIANMELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALMK
ASHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=332151 KWO_RS16575 WP_010375213.1 3575395..3577128(-) (pilB) [Xanthomonas vasicola pv. musacearum NCPPB 4379]
ATGAACGTGAGTGCATCTGCAAATTTAGTTGGTATTACCGGGATTGCGCGTCGTCTAGTCCAAGATGGCGCCCTTGAAGA
GTCTGTAGCACGGTCCGCTATGGATCAAGCTGCCTCGGCGAAAATTCCACTTCCACAGTGGTTTTCTGAAAAGAAGCTAG
TCTCAGCTGCTCAGTTGGCTGCTGCCAACGCGGTCGAGTTCGGCATGCCGTTGATCGACGTATCAGTGTTCGATGCTAGC
CAGAACGCCGTCAAGCTGGTCAGCGAAGAGTTGCTCCAAAAGCACCAAGTGCTGCCGCTGTTCAAGCGTGGCAACCGGTT
GTTCGTGGGGGTGAGCAATCCAACCCAAACCCGGGCGCTGGATGACATTAAGTTCCATACGAACTTGGTAGTTGAGGCCA
TTCTTGTTGACGAGGATCAGATCCGTCGGACACTAGAACAGTGGCAGGCCAGCAACGCGTCTTTGGGTTCGTCGCTTGGT
AATGACGACGATGAGATGGGGGACTTGGACGTCTCCACCGGCGACGAAGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAGGGGCGACGACACGCCGGTGGTGAAGTTCGTCAATAAGATGTTGGTGGATGCGATCAGGCGGGGAGCCTCGGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGCTTGCGCATCGATGGGCTGTTGAAGAACGTGGCAAAGGCGCCG
GTGAAGCTCAATCAGCGCATCGCGGCGCGCCTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCTAAGACCAAGCAGATCGACTTTCGCGTGAGCACCTTGCCAACCCTGTTCGGCGAGA
AGGTGGTGCTGCGTATCCTGGACGCCAGCGCGGCCAAGCTCGGTATCGAGAAGCTGGGCTATGAGGCTGACCAGCAGAAG
CTGTTCCTGGACGCGATCCATAAGCCTTATGGCATGGTGCTGGTGACCGGGCCGACGGGCTCGGGTAAGACGGTGTCGCT
GTATACGGCGCTGGGCATCCTCAACGACGAGACGCGGAACATTTCCACGGCAGAGGATCCGGTCGAAATTCGCTTGCCTG
GCGTCAATCAGGTGCAGCAGAACGTCAAGCGCGGCATGACCTTTGCCGCTGCGCTGCGTTCGTTCCTGCGTCAGGACCCG
GACATCATCATGGTCGGCGAAATCCGTGACCTGGAGACCGCCGAGATTGCGATCAAGGCGGCGCAGACCGGTCATATGGT
GCTGTCGACGCTGCATACCAACGATGCGCCGCAGACCATCGCGCGTCTGATGAATATGGGGATCGCGCCGTACAACATCA
CCTCGTCGGTAACGTTGGTGATCGCGCAGCGTCTGGCGCGGCGTTTGTGCAACAACTGCAAGCGCAAGTCGACGTTGCCG
GACAACGCATTGCTGGCGGAAGGTTTTACGCCCGAGCAAATCGCGAACATGGAGCTCTACGAGGCTGTGGGTTGCGACGA
GTGCACTGAAGGCTACAAGGGCCGTACCGGTATCTATCAGGTGATGCCGATGACCGACGAGATCGGCGCGATCGTGCTGG
AAGGCGGCAATGCGATGCAGATTGCCGAGGCGGCGCAGAAAATCGGTATCCGGGACTTGCGGCAGTCGGCGTTGATGAAG
GCCTCGCACGGCGTGACCAGCCTGGCTGAGATCAATCGGGTGACGAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-153)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.81

98.44

0.549
  pilB Acinetobacter baylyi ADP1

54.766

100

0.548
  pilB Legionella pneumophila strain ERS1305867

52.465

98.44

0.516
  pilF Neisseria gonorrhoeae MS11

49.12

98.44

0.484
  pilB Vibrio cholerae strain A1552

49.002

95.494

0.468
  pilB Vibrio campbellii strain DS40M4

46.397

98.614

0.458
  pilB Vibrio parahaemolyticus RIMD 2210633

45.471

97.574

0.444

Multiple sequence alignment