Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DTPSY_RS03795 Genome accession   NC_011992
Coordinates   795776..797509 (-) Length   577 a.a.
NCBI ID   WP_012655751.1    Uniprot ID   A0A6M6IAK8
Organism   [Acidovorax] ebreus TPSY     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 790776..802509
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DTPSY_RS03765 (Dtpsy_0762) - 791433..791879 (+) 447 WP_012655746.1 NUDIX domain-containing protein -
  DTPSY_RS03770 (Dtpsy_0763) - 791949..792179 (-) 231 WP_011804249.1 DNA gyrase inhibitor YacG -
  DTPSY_RS03775 (Dtpsy_0764) zapD 792186..792941 (-) 756 WP_012655747.1 cell division protein ZapD -
  DTPSY_RS03780 (Dtpsy_0765) coaE 793032..793625 (-) 594 WP_012655748.1 dephospho-CoA kinase -
  DTPSY_RS03785 (Dtpsy_0766) - 793657..794538 (-) 882 WP_012655749.1 A24 family peptidase -
  DTPSY_RS03790 (Dtpsy_0767) pilC 794538..795755 (-) 1218 WP_012655750.1 type II secretion system F family protein Machinery gene
  DTPSY_RS03795 (Dtpsy_0768) pilB 795776..797509 (-) 1734 WP_012655751.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DTPSY_RS03805 (Dtpsy_0769) - 797846..798829 (-) 984 WP_012655752.1 polyprenyl synthetase family protein -
  DTPSY_RS03810 (Dtpsy_0770) rplU 798999..799310 (+) 312 WP_012655753.1 50S ribosomal protein L21 -
  DTPSY_RS03815 (Dtpsy_0771) rpmA 799328..799585 (+) 258 WP_011804286.1 50S ribosomal protein L27 -
  DTPSY_RS03820 (Dtpsy_0772) cgtA 799732..800805 (+) 1074 WP_012655754.1 Obg family GTPase CgtA -
  DTPSY_RS03825 (Dtpsy_0773) proB 800879..802021 (+) 1143 WP_012655755.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 63629.02 Da        Isoelectric Point: 5.3559

>NTDB_id=32834 DTPSY_RS03795 WP_012655751.1 795776..797509(-) (pilB) [[Acidovorax] ebreus TPSY]
MAAIDTQKEAQPIALPGLARALMSVGKLDQKAAEEIYKKSQNSRSSFIAELTESKIITPSDLAHTVSTVFGAPLLDVDAI
DRQRIPKDLLDVKLCQSYKVIALSKRNNRLIVATADPTFQEAAEKIKFTTQMGVDWIIAEYDKLLRLIEASTKSGTETLD
GINSSNDFEFGDIPVDEAPDEDADNPAEVEDAPVVKFLHKMLLDAFNMRASDLHFEPYEHHYRVRFRIDGELREIASPPI
AIKEKLASRIKVISRLDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEPEEKER
LLQAINRPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFAVALKSFLRQDPD
IIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPNCKAPVDIPH
ETLLEAGYRDEEIDGSWVTYRPVGCSACNNGYKGRVGIYQVMPVSEEIQRIILRDGSALEIAEQARLEGVRSLRESGLHK
AKMGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=32834 DTPSY_RS03795 WP_012655751.1 795776..797509(-) (pilB) [[Acidovorax] ebreus TPSY]
ATGGCTGCTATCGACACACAGAAAGAAGCTCAACCCATTGCCTTACCCGGACTGGCGCGCGCGTTGATGTCGGTGGGCAA
ATTGGATCAAAAAGCGGCGGAAGAGATTTACAAAAAGTCACAAAATAGCCGCAGTAGCTTTATTGCAGAGCTCACGGAAT
CGAAAATCATCACGCCAAGTGATCTGGCCCATACGGTATCCACCGTATTTGGTGCCCCCTTGCTAGATGTCGATGCGATC
GACCGGCAGCGCATCCCCAAAGACCTGTTGGACGTCAAACTGTGCCAGTCCTACAAAGTCATCGCTCTCAGTAAGCGCAA
TAACCGGCTCATCGTCGCCACCGCGGATCCGACATTTCAAGAGGCCGCAGAAAAGATCAAGTTCACCACACAAATGGGGG
TGGACTGGATCATCGCGGAATATGACAAGCTGCTCCGCTTGATTGAGGCGAGCACGAAGAGCGGTACAGAAACGCTCGAC
GGCATTAACAGTAGTAATGATTTTGAGTTCGGCGACATTCCCGTTGATGAAGCTCCGGACGAGGATGCCGACAATCCCGC
GGAAGTCGAGGATGCTCCCGTTGTCAAATTTCTGCACAAGATGCTGTTGGATGCTTTCAATATGCGTGCATCCGATCTGC
ATTTTGAGCCCTACGAGCACCATTACCGTGTCCGCTTCCGCATAGATGGCGAATTGCGCGAAATCGCATCGCCGCCTATT
GCCATCAAGGAGAAGCTGGCCTCGCGCATCAAGGTGATCTCCCGCCTGGATATTTCGGAAAAACGCGTGCCCCAGGATGG
CCGCATGAAGCTCAAGGTTGGGCCGGACCGGGTTATCGATTTCCGCGTCAGCACCCTGCCTACGCTCTTTGGCGAGAAGA
TCGTGATCCGTATTCTCGATCCGAGCAGCGCCAAGCTGGGTATTGACGCACTGGGATATGAGCCCGAGGAAAAGGAGCGT
TTGCTGCAGGCCATCAACCGCCCTTATGGAATGATCTTGGTGACCGGACCCACGGGCTCCGGGAAGACCGTATCGCTCTA
CACATGTCTCAATCTGTTGAACAAGCCCGGCGTCAATATTGCGACCGCTGAGGACCCTTCGGAAATCAACCTCCCGGGTG
TGAACCAAGTCAACGTCAACGAGAAAGCCGGCCTCACGTTTGCCGTCGCGCTTAAGTCATTCCTTCGCCAAGATCCGGAC
ATCATCATGGTGGGTGAAATCCGGGATCTGGAAACGGCAGACATTTCAATCAAGGCCGCGCAGACGGGTCACTTGGTGCT
CTCTACCCTGCACACCAATGACGCTCCCACGACATTGACGCGCATGCGCAATATGGGCATCGCGCCCTTCAATATCGCAT
CCAGCGTGATTCTTATTACGGCGCAGCGACTGGCGCGTCGCTTGTGCCCCAATTGCAAGGCGCCCGTGGACATTCCGCAT
GAGACGCTGCTGGAGGCCGGCTACCGGGACGAGGAGATCGACGGCTCATGGGTTACCTACCGACCGGTGGGCTGCTCCGC
CTGCAATAACGGATACAAGGGGCGCGTGGGCATTTACCAGGTCATGCCGGTGTCCGAAGAAATCCAGCGCATCATCCTGC
GCGATGGAAGCGCACTGGAAATTGCCGAACAGGCACGCCTGGAAGGCGTCCGTTCTCTGCGTGAATCCGGGCTTCACAAG
GCAAAAATGGGGTTGACCTCGTTGGAAGAGGTCTTGGCTGTCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(51-150)

Type II/IV secretion system protein (T2SSE)

(200-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6M6IAK8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.181

99.48

0.539
  pilB Acinetobacter baumannii D1279779

53.723

97.747

0.525
  pilF Neisseria gonorrhoeae MS11

52.389

97.92

0.513
  pilB Legionella pneumophila strain ERS1305867

49.912

97.92

0.489
  pilB Vibrio parahaemolyticus RIMD 2210633

45.714

97.054

0.444
  pilB Vibrio campbellii strain DS40M4

45.455

97.227

0.442
  pilB Vibrio cholerae strain A1552

46.881

91.681

0.43
  pilF Thermus thermophilus HB27

39.897

100

0.404
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.121

92.721

0.381

Multiple sequence alignment