Detailed information NTG0326918

Bioinformatically predicted

Overview

• Name: dprA
• Type: Machinery gene
• Locus tag: EHF40_RS05390
• Genome accession: NZ_CP033907
• Coordinates: 1005488..1006324 (+)
• Length: 278 a.a.
• NCBI ID: WP_011017772.1
• Uniprot ID: A0A660A2Q7
• Organism: Streptococcus pyogenes strain Duke-Large
• Function: ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 1000488..1011324

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EHF40_RS05360 (EHF40_05360)	-	1000976..1001410 (+)	435	WP_021299262.1	hypothetical protein	-
EHF40_RS05365 (EHF40_05365)	-	1001460..1002371 (-)	912	WP_011528643.1	diacylglycerol kinase family protein	-
EHF40_RS05370 (EHF40_05370)	-	1002488..1003138 (-)	651	WP_002984629.1	hemolysin III family protein	-
EHF40_RS05375 (EHF40_05375)	-	1003135..1003575 (-)	441	WP_011017769.1	DUF1836 domain-containing protein	-
EHF40_RS05380 (EHF40_05380)	ylqF	1003794..1004642 (+)	849	WP_011017770.1	ribosome biogenesis GTPase YlqF	-
EHF40_RS05385 (EHF40_05385)	-	1004632..1005423 (+)	792	WP_011017771.1	ribonuclease HII	-
EHF40_RS05390 (EHF40_05390)	dprA	1005488..1006324 (+)	837	WP_011017772.1	DNA-processing protein DprA	Machinery gene
EHF40_RS05395 (EHF40_05395)	topA	1006431..1008560 (+)	2130	WP_011528644.1	type I DNA topoisomerase	-
EHF40_RS09900	-	1008636..1009520 (-)	885	Protein_989	LysR family transcriptional regulator	-
EHF40_RS05410 (EHF40_05410)	-	1009533..1010099 (+)	567	Protein_990	PTS sugar transporter subunit IIC	-
EHF40_RS05415 (EHF40_05415)	-	1010114..1011106 (+)	993	WP_021299266.1	D-lactate dehydrogenase	-

Sequence

Protein

• Length: 278 a.a.
• Molecular weight: 31088.08 Da
• Isoelectric Point: 9.0513

>NTDB_id=326918 EHF40_RS05390 WP_011017772.1 1005488..1006324(+) (dprA) [Streptococcus pyogenes strain Duke-Large]
MNHFELYKLKKAGLTNKNILNILDYQEHQEKSLSLRDMAVVSGCKHPSHFIEAYKQLDIQKLKMEFKQFPSISILDKHYP
MALKEIYNPPVLLFFQGNLDLLEKPKLAIVGSRRSSDTGVKSVRKILKELGNRFVIVSGLARGIDTSAHLACLKNGGQTI
AIIGTGLDRFYPKENRELQTFLGKNHLVLTEYGPREEALSYHFPERNRIIAGLSRGILVVEAKNRSGSLITCQIGIEEGR
DIFAVPGNILDGKSEGCLQLIKEGATCVTSGMDILSEY

Nucleotide

• Length: 837 bp

>NTDB_id=326918 EHF40_RS05390 WP_011017772.1 1005488..1006324(+) (dprA) [Streptococcus pyogenes strain Duke-Large]
GTGAATCATTTTGAACTTTATAAGCTAAAGAAAGCTGGACTGACTAATAAAAACATTCTCAATATTCTTGACTACCAAGA
ACACCAGGAAAAATCGTTGTCACTTCGAGATATGGCCGTTGTTTCTGGTTGTAAGCATCCGTCTCACTTTATAGAAGCCT
ATAAGCAGTTAGATATTCAAAAATTAAAAATGGAATTTAAACAATTTCCTAGTATTTCTATCTTAGATAAGCATTACCCA
ATGGCATTGAAAGAAATATACAATCCACCTGTCCTCTTGTTTTTTCAGGGAAATTTAGACCTTCTTGAGAAACCTAAATT
AGCCATTGTTGGCTCCAGACGCTCAAGCGATACCGGAGTAAAGTCTGTCCGTAAAATTCTTAAAGAACTCGGGAACCGTT
TTGTGATTGTTAGCGGACTTGCTCGAGGTATCGACACTAGTGCTCATTTAGCCTGCCTTAAAAATGGGGGACAAACCATC
GCTATTATTGGAACAGGGTTGGATCGCTTTTACCCTAAAGAAAATAGGGAGTTGCAAACTTTCTTAGGGAAAAATCATCT
TGTGCTAACAGAATACGGTCCAAGAGAAGAAGCTTTATCTTATCACTTTCCAGAACGGAATCGTATTATCGCAGGCCTTA
GCCGAGGTATTCTTGTCGTTGAAGCAAAAAATCGTTCAGGTTCCTTGATTACTTGTCAAATTGGGATAGAAGAAGGCCGA
GACATTTTTGCTGTCCCAGGAAACATTTTGGACGGGAAATCCGAAGGTTGCCTTCAGTTAATTAAAGAGGGAGCAACATG
CGTCACATCGGGCATGGATATCCTTTCAGAGTACTAA

Domains

Predicted by InterproScan.

• DNA recombination-mediator protein A (DNA_processg_A) (68-269)

• DNA processing protein A sterile alpha motif domain (SAM_DprA) (1-62)

3D structure

• AlphaFold DB: A0A660A2Q7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
dprA	Streptococcus mutans UA159	67.626	100	0.676
dprA/cilB/dalA	Streptococcus pneumoniae Rx1	60.432	100	0.604
dprA/cilB/dalA	Streptococcus pneumoniae D39	60.432	100	0.604
dprA/cilB/dalA	Streptococcus pneumoniae R6	60.432	100	0.604
dprA/cilB/dalA	Streptococcus pneumoniae TIGR4	60.432	100	0.604
dprA/cilB/dalA	Streptococcus mitis SK321	60.432	100	0.604
dprA/cilB/dalA	Streptococcus mitis NCTC 12261	60.072	100	0.601
dprA	Lactococcus lactis subsp. cremoris KW2	57.194	100	0.572
dprA	Legionella pneumophila strain ERS1305867	42.857	85.612	0.367