Detailed information NTG0325875

Bioinformatically predicted

Overview

• Name: dprA
• Type: Machinery gene
• Locus tag: EGX72_RS06845
• Genome accession: NZ_CP033809
• Coordinates: 1265169..1266011 (+)
• Length: 280 a.a.
• NCBI ID: WP_001015240.1
• Uniprot ID: Q8DZU1
• Organism: Streptococcus sp. FDAARGOS_521
• Function: ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 1260169..1271011

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EGX72_RS06820 (EGX72_06815)	-	1260463..1261011 (+)	549	WP_000136509.1	sugar O-acetyltransferase	-
EGX72_RS06825 (EGX72_06820)	-	1261250..1262212 (+)	963	WP_000735588.1	ABC transporter permease	-
EGX72_RS06830 (EGX72_06825)	-	1262209..1263183 (+)	975	WP_000588576.1	iron chelate uptake ABC transporter family permease subunit	-
EGX72_RS06835 (EGX72_06830)	-	1263180..1263941 (+)	762	WP_000614752.1	ABC transporter ATP-binding protein	-
EGX72_RS06840 (EGX72_06835)	-	1264003..1265031 (+)	1029	WP_123947881.1	siderophore ABC transporter substrate-binding protein	-
EGX72_RS06845 (EGX72_06840)	dprA	1265169..1266011 (+)	843	WP_001015240.1	DNA-processing protein DprA	Machinery gene
EGX72_RS06850 (EGX72_06845)	topA	1266106..1268226 (+)	2121	WP_000246611.1	type I DNA topoisomerase	-
EGX72_RS06855 (EGX72_06850)	-	1268363..1269064 (+)	702	WP_000120355.1	ABC transporter ATP-binding protein	-

Sequence

Protein

• Length: 280 a.a.
• Molecular weight: 31294.36 Da
• Isoelectric Point: 9.3802

>NTDB_id=325875 EGX72_RS06845 WP_001015240.1 1265169..1266011(+) (dprA) [Streptococcus sp. FDAARGOS_521]
MNHFELFKLKKAGLTNLNIHNIINYLKKNSLTSLSVRNMAVVSKCKNPTFFIENYKQLDLKKLRQEFKKFPVLSILDSNY
PLELKEIYNPPVLLFYQGNIELLSKPKLAVVGARQASQIGCQSVKKIIKETNNQFVIVSGLARGIDTAAHVSALKNGGSS
IAVIGSGLDVYYPTENKKLQEYMSYNHLVLSEYFTGEQPLKFHFPERNRIIAGLCQGIVVAEAKMRSGSLITCERALEEG
REVFAIPGNIIDGKSDGCHHLIQEGAKCIISGKDILSEYQ

Nucleotide

• Length: 843 bp

>NTDB_id=325875 EGX72_RS06845 WP_001015240.1 1265169..1266011(+) (dprA) [Streptococcus sp. FDAARGOS_521]
ATGAATCATTTTGAACTATTTAAGCTTAAAAAAGCTGGATTAACAAATCTTAATATCCACAATATTATCAACTATCTCAA
AAAGAATAGTTTAACTTCTCTATCTGTTCGCAATATGGCCGTAGTATCAAAATGTAAAAATCCTACTTTCTTTATAGAAA
ATTATAAACAGCTAGACCTTAAAAAACTTCGACAAGAATTCAAAAAATTTCCAGTTCTATCGATTTTGGATTCTAACTAT
CCTTTAGAGTTAAAAGAAATATATAATCCACCAGTTCTACTTTTTTATCAGGGTAATATTGAACTTCTATCTAAACCTAA
ATTAGCTGTAGTGGGGGCAAGACAGGCATCTCAGATAGGTTGTCAGTCTGTTAAAAAGATTATCAAGGAGACTAACAATC
AATTTGTTATCGTAAGTGGTTTAGCGCGTGGCATTGATACAGCAGCACATGTTAGTGCTTTAAAAAATGGCGGCAGTAGT
ATAGCTGTTATCGGGAGTGGTTTAGATGTTTATTATCCAACGGAGAATAAGAAACTTCAAGAATATATGTCATATAATCA
TCTCGTATTATCAGAATATTTTACCGGAGAACAACCCTTGAAATTTCATTTCCCCGAACGTAACCGTATTATTGCAGGGC
TGTGTCAAGGTATTGTGGTTGCCGAAGCTAAGATGAGATCTGGAAGTTTAATTACCTGTGAAAGAGCATTAGAAGAGGGA
CGAGAAGTTTTTGCCATTCCCGGAAATATTATCGATGGCAAATCAGATGGATGCCACCATCTTATCCAAGAAGGAGCTAA
ATGCATTATTTCAGGAAAAGATATCCTTTCTGAATATCAGTAG

Domains

Predicted by InterproScan.

• DNA recombination-mediator protein A (DNA_processg_A) (69-270)

• DNA processing protein A sterile alpha motif domain (SAM_DprA) (1-63)

3D structure

• AlphaFold DB: Q8DZU1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
dprA	Streptococcus mutans UA159	70.251	99.643	0.7
dprA/cilB/dalA	Streptococcus mitis SK321	62.5	100	0.625
dprA/cilB/dalA	Streptococcus pneumoniae Rx1	61.786	100	0.618
dprA/cilB/dalA	Streptococcus pneumoniae D39	61.786	100	0.618
dprA/cilB/dalA	Streptococcus pneumoniae R6	61.786	100	0.618
dprA/cilB/dalA	Streptococcus pneumoniae TIGR4	61.786	100	0.618
dprA/cilB/dalA	Streptococcus mitis NCTC 12261	61.429	100	0.614
dprA	Lactococcus lactis subsp. cremoris KW2	60.932	99.643	0.607
dprA	Latilactobacillus sakei subsp. sakei 23K	42.231	89.643	0.379