Detailed information NTG0325565

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: EGX83_RS13870
• Genome accession: NZ_CP033768
• Coordinates: 2899712..2900770 (-)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii strain FDAARGOS_533
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2894712..2905770

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EGX83_RS13845 (EGX83_13840)	aroK	2894987..2895556 (-)	570	WP_000643712.1	shikimate kinase AroK	-
EGX83_RS13850 (EGX83_13845)	pilQ	2895568..2897733 (-)	2166	WP_001017033.1	type IV pilus secretin PilQ family protein	Machinery gene
EGX83_RS13855 (EGX83_13850)	pilP	2897796..2898323 (-)	528	WP_000695065.1	pilus assembly protein PilP	Machinery gene
EGX83_RS13860 (EGX83_13855)	pilO	2898334..2899074 (-)	741	WP_002048088.1	type 4a pilus biogenesis protein PilO	Machinery gene
EGX83_RS13865 (EGX83_13860)	pilN	2899071..2899712 (-)	642	WP_086351977.1	PilN domain-containing protein	Machinery gene
EGX83_RS13870 (EGX83_13865)	pilM	2899712..2900770 (-)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
EGX83_RS13875 (EGX83_13870)	ponA	2900932..2903487 (+)	2556	WP_032058623.1	penicillin-binding protein PBP1a	-
EGX83_RS13880 (EGX83_13875)	-	2903505..2904329 (-)	825	WP_086351976.1	putative RNA methyltransferase	-
EGX83_RS13885 (EGX83_13880)	-	2904332..2904664 (-)	333	WP_000169602.1	hypothetical protein	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=325565 EGX83_RS13870 WP_002014066.1 2899712..2900770(-) (pilM) [Acinetobacter baumannii strain FDAARGOS_533]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=325565 EGX83_RS13870 WP_002014066.1 2899712..2900770(-) (pilM) [Acinetobacter baumannii strain FDAARGOS_533]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCGGATCGTCTTG
CGAATCCAAATCGTGTAAATGTGCTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAGTTG
GCAGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTATGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGCCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTTTTCGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGAAGAA
GCAAGCCGAGCGAAAAAAGATCGTTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGCTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTCGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCTAAGCTTTTGCAGCAAAAATTAGGTTATCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCCTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423