Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   EGX83_RS13860 Genome accession   NZ_CP033768
Coordinates   2898334..2899074 (-) Length   246 a.a.
NCBI ID   WP_002048088.1    Uniprot ID   A0A009QGI0
Organism   Acinetobacter baumannii strain FDAARGOS_533     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2893334..2904074
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGX83_RS13840 (EGX83_13835) aroB 2893876..2894958 (-) 1083 WP_001193997.1 3-dehydroquinate synthase -
  EGX83_RS13845 (EGX83_13840) aroK 2894987..2895556 (-) 570 WP_000643712.1 shikimate kinase AroK -
  EGX83_RS13850 (EGX83_13845) pilQ 2895568..2897733 (-) 2166 WP_001017033.1 type IV pilus secretin PilQ family protein Machinery gene
  EGX83_RS13855 (EGX83_13850) pilP 2897796..2898323 (-) 528 WP_000695065.1 pilus assembly protein PilP Machinery gene
  EGX83_RS13860 (EGX83_13855) pilO 2898334..2899074 (-) 741 WP_002048088.1 type 4a pilus biogenesis protein PilO Machinery gene
  EGX83_RS13865 (EGX83_13860) pilN 2899071..2899712 (-) 642 WP_086351977.1 PilN domain-containing protein Machinery gene
  EGX83_RS13870 (EGX83_13865) pilM 2899712..2900770 (-) 1059 WP_002014066.1 pilus assembly protein PilM Machinery gene
  EGX83_RS13875 (EGX83_13870) ponA 2900932..2903487 (+) 2556 WP_032058623.1 penicillin-binding protein PBP1a -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 27861.64 Da        Isoelectric Point: 4.7234

>NTDB_id=325563 EGX83_RS13860 WP_002048088.1 2898334..2899074(-) (pilO) [Acinetobacter baumannii strain FDAARGOS_533]
MSQDELQELSLEQLSAKKKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGYFIVIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
IAIEATGDYHAFGAFVSSIAALPRIVTMHDFTVDVSPAKDNKSDIPVLNYSIKAKTYRYVGATDTSEQMGNPPAASASTN
TTVQPK

Nucleotide


Download         Length: 741 bp        

>NTDB_id=325563 EGX83_RS13860 WP_002048088.1 2898334..2899074(-) (pilO) [Acinetobacter baumannii strain FDAARGOS_533]
ATGAGTCAAGACGAACTTCAAGAATTGTCATTAGAACAATTATCTGCAAAGAAAAAGAAATTTAATCTCGATAAATTCCT
TCAGCAATTTAATACGCTGGATATGAACAACTATGGTAGTTGGCCAATATCTGTAAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTGTTAGCTTTGGGGTATTTCATTGTTATTCAACCGAAATTACAAGCTATTGATAATGCTCAAGCTCAGGAA
AGCAATTTATTAAACGAATTTCGTGAAAAAGACTCGAAATTACGTAACCTGCAACAGTATCAGGTACAGCTTCAAGAGAT
GCAGGCAAATTTCAACCAGCAACTAGAGCAGTTGCCAAAAGAAACTGAGATTCCGAGTTTAGTTGAAGATATCAACTTAA
CAGGAGTGAACTCTGGATTGAAATTTAAAAATATTCGCTTAGAAGATGAAGTAAAGCAGGAAATTTTTATTGAGCAACCA
ATTGCCATAGAAGCGACAGGGGACTATCACGCATTTGGAGCTTTTGTAAGTAGTATCGCTGCTTTGCCTCGTATTGTAAC
CATGCACGATTTTACTGTTGATGTATCGCCAGCTAAAGATAACAAGTCTGATATTCCTGTGCTGAACTATTCCATTAAAG
CAAAAACATACCGATACGTAGGGGCAACAGATACATCGGAGCAGATGGGCAACCCTCCAGCAGCATCTGCTTCAACGAAC
ACTACTGTTCAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A009QGI0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

98.78

100

0.988
  comO Acinetobacter baylyi ADP1

69.136

98.78

0.683

Multiple sequence alignment