Detailed information NTG0324378

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: EFV54_RS09390
• Genome accession: NZ_CP033607
• Coordinates: 1832857..1833504 (-)
• Length: 215 a.a.
• NCBI ID: WP_010906131.1
• Uniprot ID: A0A3N6KQH1
• Organism: Lactococcus lactis strain IL1403
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1827857..1838504

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EFV54_RS09365 (EFV54_09365)	atpF	1827927..1828433 (-)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-
EFV54_RS09370 (EFV54_09370)	atpB	1828448..1829161 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
EFV54_RS09375 (EFV54_09375)	-	1829206..1829421 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
EFV54_RS09380 (EFV54_09380)	-	1829605..1830381 (-)	777	WP_004255244.1	alpha/beta hydrolase family protein	-
EFV54_RS09385 (EFV54_09385)	comEC	1830666..1832876 (-)	2211	WP_010906130.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
EFV54_RS09390 (EFV54_09390)	comEA	1832857..1833504 (-)	648	WP_010906131.1	ComEA family DNA-binding protein	Machinery gene
EFV54_RS09395 (EFV54_09395)	-	1833564..1834925 (-)	1362	WP_010906132.1	ABC transporter permease	-
EFV54_RS09400 (EFV54_09400)	-	1834922..1835854 (-)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
EFV54_RS09405 (EFV54_09405)	-	1835959..1836357 (-)	399	WP_004255236.1	hypothetical protein	-
EFV54_RS09410 (EFV54_09410)	-	1836461..1837024 (-)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
EFV54_RS09415 (EFV54_09415)	-	1837197..1838375 (-)	1179	WP_010906133.1	SLC13 family permease	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23624.76 Da
• Isoelectric Point: 4.8659

>NTDB_id=324378 EFV54_RS09390 WP_010906131.1 1832857..1833504(-) (comEA) [Lactococcus lactis strain IL1403]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=324378 EFV54_RS09390 WP_010906131.1 1832857..1833504(-) (comEA) [Lactococcus lactis strain IL1403]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATTTAATTAAAGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (151-212)

3D structure

• AlphaFold DB: A0A3N6KQH1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	76.147	100	0.772
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	36.564	100	0.386