Detailed information NTG0324328

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: EFD52_RS08215
• Genome accession: NZ_CP033606
• Coordinates: 1689799..1690446 (-)
• Length: 215 a.a.
• NCBI ID: WP_010906131.1
• Uniprot ID: A0A3N6KQH1
• Organism: Lactococcus lactis strain IL6288
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1684799..1695446

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EFD52_RS08190 (EFD52_08190)	atpF	1684869..1685375 (-)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-
EFD52_RS08195 (EFD52_08195)	atpB	1685390..1686103 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
EFD52_RS08200 (EFD52_08200)	-	1686148..1686363 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
EFD52_RS08205 (EFD52_08205)	-	1686547..1687323 (-)	777	WP_004255244.1	alpha/beta hydrolase family protein	-
EFD52_RS08210 (EFD52_08210)	comEC	1687608..1689818 (-)	2211	WP_010906130.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
EFD52_RS08215 (EFD52_08215)	comEA	1689799..1690446 (-)	648	WP_010906131.1	ComEA family DNA-binding protein	Machinery gene
EFD52_RS08220 (EFD52_08220)	-	1690506..1691867 (-)	1362	WP_010906132.1	ABC transporter permease	-
EFD52_RS08225 (EFD52_08225)	-	1691864..1692796 (-)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
EFD52_RS08230 (EFD52_08230)	-	1692901..1693299 (-)	399	WP_004255236.1	hypothetical protein	-
EFD52_RS08235 (EFD52_08235)	-	1693403..1693966 (-)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
EFD52_RS08240 (EFD52_08240)	-	1694139..1695317 (-)	1179	WP_010906133.1	SLC13 family permease	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23624.76 Da
• Isoelectric Point: 4.8659

>NTDB_id=324328 EFD52_RS08215 WP_010906131.1 1689799..1690446(-) (comEA) [Lactococcus lactis strain IL6288]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=324328 EFD52_RS08215 WP_010906131.1 1689799..1690446(-) (comEA) [Lactococcus lactis strain IL6288]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATTTAATTAAAGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (151-212)

3D structure

• AlphaFold DB: A0A3N6KQH1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	76.147	100	0.772
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	36.564	100	0.386