Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ECB94_RS06895 Genome accession   NZ_CP033577
Coordinates   1490493..1491719 (+) Length   408 a.a.
NCBI ID   WP_124940290.1    Uniprot ID   A0A3G4V8F0
Organism   Vibrio mediterranei strain 117-T6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1485493..1496719
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ECB94_RS06875 (ECB94_06885) ampD 1486460..1487044 (-) 585 WP_006074431.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ECB94_RS06880 (ECB94_06890) nadC 1487180..1488070 (+) 891 WP_124940288.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ECB94_RS06885 (ECB94_06895) - 1488312..1488791 (+) 480 WP_006074427.1 pilin -
  ECB94_RS06890 (ECB94_06900) pilB 1488791..1490482 (+) 1692 WP_164491468.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ECB94_RS06895 (ECB94_06905) pilC 1490493..1491719 (+) 1227 WP_124940290.1 type II secretion system F family protein Machinery gene
  ECB94_RS06900 (ECB94_06910) pilD 1491762..1492631 (+) 870 WP_124940291.1 prepilin peptidase Machinery gene
  ECB94_RS06905 (ECB94_06915) coaE 1492634..1493242 (+) 609 WP_006074421.1 dephospho-CoA kinase -
  ECB94_RS06910 (ECB94_06920) zapD 1493274..1494014 (+) 741 WP_031494891.1 cell division protein ZapD -
  ECB94_RS06915 (ECB94_06925) yacG 1494036..1494230 (+) 195 WP_006074419.1 DNA gyrase inhibitor YacG -
  ECB94_RS06920 (ECB94_06930) rplS 1494434..1494787 (-) 354 WP_006074418.1 50S ribosomal protein L19 -
  ECB94_RS06925 (ECB94_06935) trmD 1494830..1495579 (-) 750 WP_038224311.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  ECB94_RS06930 (ECB94_06940) rimM 1495614..1496171 (-) 558 WP_031494889.1 ribosome maturation factor RimM -
  ECB94_RS06935 (ECB94_06945) rpsP 1496192..1496440 (-) 249 WP_031494887.1 30S ribosomal protein S16 -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 45105.11 Da        Isoelectric Point: 10.1763

>NTDB_id=324104 ECB94_RS06895 WP_124940290.1 1490493..1491719(+) (pilC) [Vibrio mediterranei strain 117-T6]
MPNPLPMPLRKFRWRGVDRSGAKAKGQILALTEMEVRNQLGRQQIHIRKISRHRISFWTRLTHKISRKDITLFTRQLATL
LTTRVPLVQALKLISESHSKAEMKSVLNQVASRVESGVPIAQALQALSTHFDRFYIDMIATGESSGNIAVTLDRLATYRE
KHDALQSKVVKAMIYPSMVLIVSCAVTYLMLTSVIPEFESMFKGFNANLPWFTSEVLELSNWSQSYGLVSVASLGLLIAS
IKFISLKNATAELYISKLAIKLPVVGDIIAKATIAKFSRTLATSFSSGIPILTGIQASARTTENAYFQQAIFSIHTHVVA
GVPVHLAMRNSLAFPEMVLQMVMIGEETGKLDEMLNKIASIYEADVDNIVDNLGTIIEPLVIVFLGTIIGGLVIAMYLPI
FNLMSVIG

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=324104 ECB94_RS06895 WP_124940290.1 1490493..1491719(+) (pilC) [Vibrio mediterranei strain 117-T6]
ATGCCTAACCCGCTTCCTATGCCATTAAGAAAGTTCCGCTGGAGAGGTGTTGATCGATCTGGCGCAAAAGCAAAGGGGCA
AATCCTTGCTTTAACCGAAATGGAGGTCAGAAATCAATTAGGTCGGCAGCAAATCCATATCAGAAAAATTTCTCGCCATC
GCATTTCGTTTTGGACCAGATTAACCCACAAGATTTCACGCAAAGACATCACTCTATTCACTAGGCAATTGGCTACTTTG
CTGACGACAAGAGTTCCTCTTGTTCAAGCCCTTAAGCTTATCTCTGAAAGCCACTCCAAAGCCGAAATGAAATCGGTCCT
TAATCAGGTCGCTAGCCGAGTGGAGAGTGGCGTCCCTATCGCTCAAGCGCTACAAGCACTAAGTACACATTTTGACCGCT
TCTATATCGATATGATTGCGACGGGTGAATCGTCCGGAAACATTGCGGTGACGTTAGATAGGTTAGCAACATATCGTGAA
AAACATGATGCTCTGCAGTCTAAAGTCGTTAAAGCCATGATATATCCAAGTATGGTGCTGATAGTTTCTTGTGCCGTAAC
CTATTTGATGCTCACGAGTGTGATTCCTGAATTTGAATCGATGTTTAAAGGGTTCAATGCCAATTTACCTTGGTTCACAT
CCGAGGTGCTGGAGCTATCAAACTGGAGCCAATCATACGGACTGGTTAGCGTTGCTTCTCTAGGTTTATTGATTGCTAGC
ATTAAGTTTATTAGCCTTAAAAATGCTACTGCCGAATTATATATAAGTAAATTAGCCATCAAACTGCCCGTTGTTGGCGA
CATCATTGCCAAAGCAACCATTGCCAAATTTAGCCGAACATTAGCGACCAGTTTTAGTTCTGGAATACCGATACTCACAG
GCATACAAGCGAGCGCAAGAACAACAGAGAACGCTTACTTTCAACAAGCTATTTTTTCTATACACACTCATGTCGTAGCG
GGCGTACCTGTTCATTTAGCAATGCGAAACAGTCTCGCTTTCCCTGAGATGGTGCTGCAAATGGTCATGATAGGTGAAGA
AACTGGCAAGTTAGATGAAATGCTCAATAAGATCGCTAGCATCTATGAGGCGGATGTCGATAACATCGTAGACAACTTAG
GAACAATCATCGAGCCTTTGGTTATTGTCTTCCTTGGCACAATAATTGGCGGGTTAGTGATTGCAATGTACTTACCTATC
TTTAACTTAATGTCGGTAATAGGCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(277-399)

Type II secretion system (T2SS), protein F (T2SSF)

(73-196)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3G4V8F0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio cholerae strain A1552

56.219

98.529

0.554
  pilC Vibrio campbellii strain DS40M4

55.112

98.284

0.542
  pilC Acinetobacter baylyi ADP1

41.045

98.529

0.404
  pilC Acinetobacter baumannii D1279779

39.85

97.794

0.39
  pilC Pseudomonas stutzeri DSM 10701

39.447

97.549

0.385
  pilC Legionella pneumophila strain ERS1305867

37.811

98.529

0.373

Multiple sequence alignment