Detailed information NTG0323459

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: JL719_RS02525
• Genome accession: NZ_CP033381
• Coordinates: 566046..567203 (+)
• Length: 385 a.a.
• NCBI ID: WP_033156117.1
• Uniprot ID: A0A2N0VAW9
• Organism: Methylomonas sp. LW13
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 561046..572203

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JL719_RS02510	-	562076..562498 (-)	423	WP_129871332.1	hypothetical protein	-
JL719_RS02515 (U737_02565)	-	562640..563272 (-)	633	WP_033155855.1	response regulator transcription factor	-
JL719_RS02520 (U737_02570)	-	563269..565857 (-)	2589	WP_051669754.1	PAS domain S-box protein	-
JL719_RS02525 (U737_02575)	pilU	566046..567203 (+)	1158	WP_033156117.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
JL719_RS02530 (U737_02580)	-	567440..567763 (-)	324	WP_026602326.1	NGG1p interacting factor 3 protein, NIF3	-
JL719_RS02535	-	567812..567961 (-)	150	WP_165385910.1	hypothetical protein	-
JL719_RS02540 (U737_02585)	lexA	567971..568600 (-)	630	WP_033155856.1	transcriptional repressor LexA	-
JL719_RS02545 (U737_02590)	-	569005..569265 (-)	261	WP_033155857.1	hypothetical protein	-
JL719_RS23945	-	569449..569655 (-)	207	WP_225892983.1	winged helix-turn-helix domain-containing protein	-
JL719_RS23950	-	569654..570025 (+)	372	WP_225892984.1	hypothetical protein	-
JL719_RS02555 (U737_02600)	-	570185..570904 (-)	720	WP_033155859.1	cytochrome c1	-

Sequence

Protein

• Length: 385 a.a.
• Molecular weight: 42478.71 Da
• Isoelectric Point: 6.2424

>NTDB_id=323459 JL719_RS02525 WP_033156117.1 566046..567203(+) (pilU) [Methylomonas sp. LW13]
MEFKDYLKILVQHDGSDLYLTAGAPPAAKFQGSLKPLENVKLSRERIKEIADGIMDAEQRAAFEQVPEMNLAITEEGIGR
FRVNIFKQRNCHALVIRNIKVDIPSADALGLPQILKDKIMEKRGLILFVGGTGSGKSTSLAALIDYRNSNSSGHIITIED
PIEFVHPHKKSLVNQREVGVDTLSYEDALKNTLRQAPDVILIGEIRSQETMEHALAFAETGHLCLSTLHANNANQALDRI
INFFPEERRPQLLMDLSLNLQAFISQRLVPTVEGKRVAAIEILLGTKLVSDLIHKGDIHAIKEAMEKSENIGMQTFDSHL
LKLYKGGVITLEEALRNSDSPNNLKLKINLSEGLGSATKKPSSLAGSLALEEITKKEGDEESEGH

Nucleotide

• Length: 1158 bp

>NTDB_id=323459 JL719_RS02525 WP_033156117.1 566046..567203(+) (pilU) [Methylomonas sp. LW13]
ATGGAATTTAAAGACTATCTAAAAATCCTCGTCCAACACGATGGTTCGGACCTTTACCTGACGGCGGGCGCTCCACCGGC
GGCCAAGTTCCAAGGCAGCTTAAAACCGTTGGAAAACGTCAAACTTAGCCGTGAACGCATCAAGGAAATTGCTGACGGTA
TCATGGACGCCGAGCAACGCGCCGCCTTCGAACAGGTGCCGGAAATGAATCTGGCGATCACCGAAGAAGGAATCGGACGG
TTTCGGGTGAATATTTTTAAACAGCGCAATTGCCATGCCTTGGTAATTCGCAACATCAAGGTCGATATTCCCAGTGCCGA
CGCCTTGGGCTTGCCGCAAATCCTCAAAGATAAAATCATGGAAAAGCGCGGGCTGATTTTGTTCGTTGGCGGCACCGGTT
CCGGTAAATCGACGTCCTTGGCGGCCTTGATAGATTATCGCAATAGCAACTCATCCGGGCATATCATTACCATCGAAGAT
CCTATCGAGTTTGTGCATCCGCATAAAAAATCCTTGGTCAATCAACGCGAAGTCGGTGTCGATACCCTCAGTTATGAGGA
TGCATTGAAAAACACTTTGCGGCAGGCGCCGGATGTGATTCTGATCGGCGAAATTCGCAGTCAGGAGACCATGGAACACG
CTTTGGCTTTTGCCGAAACCGGGCATTTGTGCTTGTCCACGTTACACGCCAATAACGCCAATCAGGCCTTGGACCGGATC
ATCAACTTTTTCCCGGAAGAGCGCCGTCCGCAGTTGTTGATGGACTTGTCCTTGAATCTGCAAGCGTTTATTTCCCAACG
CCTGGTACCCACCGTGGAAGGTAAGCGGGTGGCGGCTATCGAAATTTTGCTGGGTACTAAATTGGTCAGCGATTTGATCC
ATAAAGGCGATATTCACGCCATTAAGGAAGCGATGGAAAAATCCGAAAATATCGGCATGCAGACTTTTGACAGCCATTTA
CTCAAGCTCTACAAAGGCGGGGTGATCACCTTGGAAGAGGCCTTGCGCAACTCGGATTCGCCGAACAATTTAAAACTGAA
AATCAATTTGAGCGAAGGTTTGGGGTCGGCGACCAAAAAGCCCAGTTCTTTGGCTGGCAGTCTTGCTTTGGAAGAAATCA
CCAAGAAAGAGGGGGATGAGGAAAGTGAGGGACATTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (60-270)

3D structure

• AlphaFold DB: A0A2N0VAW9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.684	97.143	0.551
pilU	Acinetobacter baylyi ADP1	55.367	91.948	0.509
pilU	Vibrio cholerae strain A1552	49.718	91.948	0.457
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.096	94.805	0.39
pilT	Legionella pneumophila strain Lp02	42.727	85.714	0.366
pilT	Legionella pneumophila strain ERS1305867	42.727	85.714	0.366