Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FDP16_RS11220 Genome accession   NZ_CP040798
Coordinates   2243318..2245000 (-) Length   560 a.a.
NCBI ID   WP_176799686.1    Uniprot ID   -
Organism   Streptococcus sanguinis strain CGMH058     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2238318..2250000
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FDP16_RS11190 (FDP16_11670) - 2238754..2239215 (-) 462 WP_176799822.1 hypothetical protein -
  FDP16_RS11195 (FDP16_11675) - 2239374..2239817 (-) 444 WP_176799682.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FDP16_RS11200 (FDP16_11680) - 2239858..2240301 (-) 444 WP_176799683.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FDP16_RS11205 (FDP16_11685) - 2240406..2240849 (-) 444 WP_176799684.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FDP16_RS11210 (FDP16_11690) pilC 2241022..2242248 (-) 1227 WP_176799685.1 type II secretion system F family protein Machinery gene
  FDP16_RS11215 (FDP16_11695) pilT 2242252..2243316 (-) 1065 WP_002899359.1 type IV pilus twitching motility protein PilT Machinery gene
  FDP16_RS11220 (FDP16_11700) pilB 2243318..2245000 (-) 1683 WP_176799686.1 GspE/PulE family protein Machinery gene
  FDP16_RS11225 (FDP16_11705) - 2246120..2247007 (-) 888 WP_176799687.1 cation diffusion facilitator family transporter -
  FDP16_RS11230 (FDP16_11710) - 2247177..2247698 (+) 522 WP_176799688.1 TetR/AcrR family transcriptional regulator -
  FDP16_RS11235 (FDP16_11715) - 2247737..2248903 (+) 1167 WP_176799689.1 MFS transporter -
  FDP16_RS11240 (FDP16_11720) - 2249055..2249882 (+) 828 WP_176799690.1 Cof-type HAD-IIB family hydrolase -

Sequence

Protein


Download         Length: 560 a.a.        Molecular weight: 62211.34 Da        Isoelectric Point: 4.9442

>NTDB_id=323358 FDP16_RS11220 WP_176799686.1 2243318..2245000(-) (pilB) [Streptococcus sanguinis strain CGMH058]
MALIAILVQFNLITAAQKEEILQDMPQSNMQLERYLISKGYVTEEDMLKVMSYYYRVPHVNLSQFVIEKEAVEKVSEKVA
KRHGLIPISFTDGEEGEDPKLIVAMADPSNYIALDDVKIVSKMAVEPYVTFRDDIEKYIDQYYSKGEEAQQAATEIEGFN
MDEEIVEEDLEIKNAPVVRLIDSIISQAIKTRTSDIHIEPFEKVVRVRFRVDGTLVENMQLKANAHSAIATRIKIMSGLD
IAERRIPQDGRIETTIDGKEVDMRVSVLPTVFGEKIVIRILSRNATLLSKEELGFSPTNQKLFEDILKAPEGIILLTGPT
GSGKTTTLYTALRELNDVGKNIITVEDPVEYRLEGVNQVQVNNKAGLTFASGLRSILRQDPDIVLLGEIRDEETASIAVR
AAITGHVVLSTIHTNDTASTVNRLVDMGIKPYLVSTATVGIIAQRLIKRICPKCKTEYTVETNEHAGIGIHKGDTLYRGR
GCNYCSGTGYYGRIAIHEIMAVTREIKSLINDGGTTAQLRAEAKKNGMRDLAEEAIDIAKQGTTTIEEAMKIAFSLEGEV

Nucleotide


Download         Length: 1683 bp        

>NTDB_id=323358 FDP16_RS11220 WP_176799686.1 2243318..2245000(-) (pilB) [Streptococcus sanguinis strain CGMH058]
ATGGCACTAATCGCTATATTGGTTCAATTCAATCTGATAACGGCAGCTCAAAAAGAAGAAATTCTTCAAGATATGCCACA
ATCAAACATGCAGTTAGAGAGATATCTAATCAGCAAGGGCTATGTGACAGAAGAAGATATGCTGAAAGTCATGAGTTACT
ATTATCGTGTACCTCATGTCAATCTATCACAGTTTGTGATAGAAAAGGAAGCTGTCGAGAAGGTTTCAGAAAAAGTTGCT
AAACGTCATGGATTGATTCCGATCTCTTTCACAGATGGAGAAGAGGGAGAAGACCCCAAGTTAATAGTTGCGATGGCAGA
CCCAAGCAACTATATCGCCCTAGACGATGTTAAAATCGTTTCTAAGATGGCGGTAGAACCTTATGTAACTTTTCGGGATG
ATATTGAGAAGTATATCGATCAATACTATTCCAAAGGAGAAGAAGCCCAACAGGCAGCGACCGAAATCGAAGGTTTTAAT
ATGGATGAAGAGATCGTCGAAGAAGATCTCGAAATCAAAAACGCTCCGGTTGTACGTTTGATTGACTCGATTATCAGTCA
GGCAATCAAGACGCGGACCAGCGATATCCATATTGAGCCCTTTGAAAAAGTTGTTCGTGTTCGTTTCCGGGTTGACGGAA
CCTTGGTGGAAAACATGCAGTTGAAAGCCAATGCTCACTCAGCCATTGCGACACGGATTAAGATCATGAGTGGTCTGGAC
ATTGCCGAGCGGCGGATTCCTCAGGATGGACGGATTGAAACAACCATCGACGGCAAAGAAGTCGACATGCGGGTTTCAGT
CTTGCCTACTGTATTCGGTGAAAAAATCGTTATTCGGATTTTGAGTCGGAATGCAACTCTTCTCAGTAAAGAGGAGCTGG
GATTCTCGCCAACTAATCAGAAGCTCTTTGAAGATATTCTTAAGGCTCCAGAAGGCATTATCTTGCTGACTGGTCCGACA
GGAAGCGGAAAGACAACCACTCTCTACACAGCGCTCCGTGAGCTCAATGATGTAGGAAAAAATATCATCACTGTTGAAGA
TCCGGTTGAGTACCGATTGGAAGGGGTCAACCAAGTTCAGGTAAATAATAAAGCTGGACTGACCTTCGCGAGTGGTTTGA
GAAGTATCCTGCGTCAGGACCCGGACATCGTTCTTTTGGGGGAAATTCGGGACGAAGAGACAGCTAGTATCGCGGTTCGG
GCCGCTATCACTGGTCACGTCGTTCTCTCGACTATCCACACCAATGACACAGCTAGTACAGTCAACCGTTTGGTCGATAT
GGGCATCAAGCCTTATCTGGTCTCAACGGCGACTGTCGGTATTATCGCACAGCGCTTGATTAAGCGCATCTGTCCTAAGT
GTAAGACTGAATATACTGTAGAAACAAATGAACATGCAGGTATCGGTATTCACAAGGGAGACACCTTGTATCGCGGACGC
GGCTGCAACTACTGCAGCGGTACGGGATACTATGGACGTATAGCTATCCACGAAATCATGGCAGTGACAAGGGAGATTAA
ATCACTAATTAATGATGGTGGAACAACAGCACAGCTTCGAGCAGAAGCTAAGAAAAATGGTATGCGAGACTTGGCGGAGG
AGGCCATTGATATCGCAAAACAGGGGACTACAACGATTGAAGAAGCAATGAAGATTGCCTTTAGTCTAGAAGGGGAAGTT
TAG

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(184-448)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(36-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

38.693

100

0.391
  pilB Vibrio parahaemolyticus RIMD 2210633

38.693

100

0.391
  pilF Thermus thermophilus HB27

39.35

98.929

0.389
  pilB Vibrio campbellii strain DS40M4

38.272

100

0.388
  pilB Legionella pneumophila strain ERS1305867

38.324

100

0.384
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.241

99.464

0.38
  pilF Neisseria gonorrhoeae MS11

37.814

99.643

0.377
  pilB Acinetobacter baylyi ADP1

36.972

100

0.375
  pilB Acinetobacter baumannii D1279779

36.918

99.643

0.368