Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   EB818_RS00665 Genome accession   NZ_CP033335
Coordinates   102895..103929 (+) Length   344 a.a.
NCBI ID   WP_228631569.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain TSPY208     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 97895..108929
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EB818_RS00655 (EB818_00655) - 101563..101928 (+) 366 WP_002986560.1 DUF1033 family protein -
  EB818_RS00660 (EB818_00660) comYA 102021..102959 (+) 939 WP_002986557.1 competence type IV pilus ATPase ComGA Machinery gene
  EB818_RS00665 (EB818_00665) comYB 102895..103929 (+) 1035 WP_228631569.1 competence type IV pilus assembly protein ComGB Machinery gene
  EB818_RS00670 (EB818_00670) comYC 103931..104257 (+) 327 WP_011528161.1 competence type IV pilus major pilin ComGC Machinery gene
  EB818_RS00675 (EB818_00675) comGD 104232..104660 (+) 429 WP_076639399.1 competence type IV pilus minor pilin ComGD -
  EB818_RS00680 (EB818_00680) comGE 104617..104901 (+) 285 WP_002987779.1 competence type IV pilus minor pilin ComGE -
  EB818_RS00685 (EB818_00685) comYF 104894..105328 (+) 435 WP_002986542.1 competence type IV pilus minor pilin ComGF Machinery gene
  EB818_RS00690 (EB818_00690) comGG 105312..105638 (+) 327 WP_002986539.1 competence type IV pilus minor pilin ComGG -
  EB818_RS00695 (EB818_00695) comYH 105736..106689 (+) 954 WP_129322573.1 class I SAM-dependent methyltransferase Machinery gene
  EB818_RS00700 (EB818_00700) - 106748..107944 (+) 1197 WP_020904834.1 acetate kinase -
  EB818_RS00705 (EB818_00705) - 108131..108439 (+) 309 Protein_88 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 39648.10 Da        Isoelectric Point: 9.7085

>NTDB_id=322996 EB818_RS00665 WP_228631569.1 102895..103929(+) (comYB) [Streptococcus pyogenes strain TSPY208]
MISFLRQDISIPSKLSLKKLSSKHQHKFIQLLANLLSTGFSFAEVIAFLKRSQLLQLDYVLKMEESLLKGQGLADMLSGL
GFSDAILTQISLADRHGNIETTLVAIQHYLNQMARIRRKTAEVITYPLILLLFLFVMMLGLRRYLVPQLETQNQITYFLN
HFPAFFIGFCSGLILLFGMVWLRWRSQSRLKLYSRLSRYPFLGKLLKQYLTSYYAREWGTLIGQGLDLMTILDIMAIEKS
SLMKELAEDIRMSLLEGQAFHIKVATYPFFKKELSLMIEYGEIKSKLGAELEIYAQESWEQFFSQLYQVTQLIQPAIFLV
VAVTIVMIYAAILLPIYQNMGGIF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=322996 EB818_RS00665 WP_228631569.1 102895..103929(+) (comYB) [Streptococcus pyogenes strain TSPY208]
TTGATCAGCTTCTTGAGGCAGGACATCTCAATCCCAAGCAAGCTAAGCTTGAAAAAATTATCTAGTAAACATCAGCATAA
ATTCATTCAATTACTAGCTAATCTTTTATCTACAGGATTTAGCTTCGCAGAGGTTATTGCTTTTTTAAAAAGAAGCCAGT
TGCTTCAGCTAGATTATGTTCTTAAAATGGAAGAGTCTTTATTAAAAGGACAAGGCTTAGCAGATATGTTGTCGGGTTTA
GGTTTTTCAGATGCTATTCTTACTCAAATTAGTTTAGCTGATAGACACGGTAATATTGAAACAACATTAGTAGCGATTCA
ACATTACTTGAACCAAATGGCAAGGATCAGACGAAAAACTGCTGAAGTTATCACCTATCCTCTTATTCTTTTACTTTTTC
TTTTTGTGATGATGCTAGGGTTGAGACGCTATTTAGTCCCTCAATTAGAAACTCAAAATCAGATAACTTACTTTCTTAAC
CATTTTCCTGCCTTCTTTATAGGTTTTTGCTCAGGTCTTATTTTGTTGTTCGGGATGGTATGGCTACGATGGCGATCTCA
AAGCCGCTTGAAACTCTATAGTCGGCTAAGCCGTTATCCTTTTTTAGGTAAACTGTTAAAACAATACTTAACTAGTTATT
ATGCTAGAGAATGGGGTACGTTAATAGGTCAAGGTCTTGATTTGATGACTATTTTAGACATTATGGCCATTGAGAAATCC
TCACTAATGAAAGAATTAGCTGAGGACATCAGAATGAGTCTGCTTGAAGGGCAAGCCTTTCACATTAAAGTAGCTACCTA
CCCTTTTTTTAAAAAAGAATTAAGTTTAATGATTGAATACGGTGAAATCAAATCGAAACTGGGGGCCGAGTTGGAAATCT
ATGCCCAGGAAAGCTGGGAACAATTTTTTAGTCAGCTTTATCAAGTGACACAACTTATTCAACCAGCTATTTTTTTAGTA
GTTGCTGTGACAATCGTCATGATTTATGCGGCAATCCTATTACCAATTTACCAAAATATGGGAGGTATTTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(28-147)

Type II secretion system (T2SS), protein F (T2SSF)

(215-335)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

53.687

98.547

0.529
  comYB Streptococcus mutans UA159

53.687

98.547

0.529
  comYB Streptococcus gordonii str. Challis substr. CH1

50.588

98.837

0.5
  comGB/cglB Streptococcus mitis NCTC 12261

47.619

97.674

0.465
  comGB/cglB Streptococcus mitis SK321

47.748

96.802

0.462
  comGB/cglB Streptococcus pneumoniae Rx1

45.646

96.802

0.442
  comGB/cglB Streptococcus pneumoniae D39

45.646

96.802

0.442
  comGB/cglB Streptococcus pneumoniae R6

45.646

96.802

0.442
  comGB/cglB Streptococcus pneumoniae TIGR4

45.646

96.802

0.442
  comGB Lactococcus lactis subsp. cremoris KW2

42.769

94.477

0.404

Multiple sequence alignment