Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYF   Type   Machinery gene
Locus tag   EB818_RS00685 Genome accession   NZ_CP033335
Coordinates   104894..105328 (+) Length   144 a.a.
NCBI ID   WP_002986542.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain TSPY208     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 99894..110328
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EB818_RS00655 (EB818_00655) - 101563..101928 (+) 366 WP_002986560.1 DUF1033 family protein -
  EB818_RS00660 (EB818_00660) comYA 102021..102959 (+) 939 WP_002986557.1 competence type IV pilus ATPase ComGA Machinery gene
  EB818_RS00665 (EB818_00665) comYB 102895..103929 (+) 1035 WP_228631569.1 competence type IV pilus assembly protein ComGB Machinery gene
  EB818_RS00670 (EB818_00670) comYC 103931..104257 (+) 327 WP_011528161.1 competence type IV pilus major pilin ComGC Machinery gene
  EB818_RS00675 (EB818_00675) comGD 104232..104660 (+) 429 WP_076639399.1 competence type IV pilus minor pilin ComGD -
  EB818_RS00680 (EB818_00680) comGE 104617..104901 (+) 285 WP_002987779.1 competence type IV pilus minor pilin ComGE -
  EB818_RS00685 (EB818_00685) comYF 104894..105328 (+) 435 WP_002986542.1 competence type IV pilus minor pilin ComGF Machinery gene
  EB818_RS00690 (EB818_00690) comGG 105312..105638 (+) 327 WP_002986539.1 competence type IV pilus minor pilin ComGG -
  EB818_RS00695 (EB818_00695) comYH 105736..106689 (+) 954 WP_129322573.1 class I SAM-dependent methyltransferase Machinery gene
  EB818_RS00700 (EB818_00700) - 106748..107944 (+) 1197 WP_020904834.1 acetate kinase -
  EB818_RS00705 (EB818_00705) - 108131..108439 (+) 309 Protein_88 hypothetical protein -
  EB818_RS00710 (EB818_00710) proC 108522..109292 (-) 771 WP_076639397.1 pyrroline-5-carboxylate reductase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16573.20 Da        Isoelectric Point: 10.3584

>NTDB_id=322998 EB818_RS00685 WP_002986542.1 104894..105328(+) (comYF) [Streptococcus pyogenes strain TSPY208]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIGQSQICITLKWKSGLERTFYYAFQD

Nucleotide


Download         Length: 435 bp        

>NTDB_id=322998 EB818_RS00685 WP_002986542.1 104894..105328(+) (comYF) [Streptococcus pyogenes strain TSPY208]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACCCTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCAAACCCTCCTTAAACGTAGCCATTATCTAGCCCGTCATGATCAAGACAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGGAGCAAGATTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAG
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTCCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGGGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(41-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

51.079

96.528

0.493
  comYF Streptococcus mutans UA159

50.36

96.528

0.486
  comGF Lactococcus lactis subsp. cremoris KW2

45.985

95.139

0.437
  comGF/cglF Streptococcus mitis NCTC 12261

44.03

93.056

0.41
  comGF/cglF Streptococcus pneumoniae Rx1

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae D39

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae R6

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae TIGR4

43.609

92.361

0.403
  comGF/cglF Streptococcus mitis SK321

42.857

92.361

0.396

Multiple sequence alignment