Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   D9T18_RS14150 Genome accession   NZ_CP033065
Coordinates   3135654..3137330 (+) Length   558 a.a.
NCBI ID   WP_029773362.1    Uniprot ID   A0AAD0U4F7
Organism   Pseudoalteromonas agarivorans strain Hao 2018     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3130654..3142330
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D9T18_RS14125 (D9T18_14125) nadC 3130741..3131586 (+) 846 WP_121638013.1 carboxylating nicotinate-nucleotide diphosphorylase -
  D9T18_RS14130 (D9T18_14130) - 3132061..3132498 (+) 438 WP_121638014.1 pilin -
  D9T18_RS14135 (D9T18_14135) pilC 3132617..3133846 (+) 1230 WP_121638015.1 type II secretion system F family protein Machinery gene
  D9T18_RS14140 (D9T18_14140) pilD 3133995..3134885 (+) 891 WP_121638016.1 prepilin peptidase Machinery gene
  D9T18_RS14145 (D9T18_14145) coaE 3134887..3135507 (+) 621 WP_121638017.1 dephospho-CoA kinase -
  D9T18_RS14150 (D9T18_14150) pilB 3135654..3137330 (+) 1677 WP_029773362.1 type IV-A pilus assembly ATPase PilB Machinery gene
  D9T18_RS14155 (D9T18_14155) yacG 3137378..3137608 (+) 231 WP_024031401.1 DNA gyrase inhibitor YacG -
  D9T18_RS14160 (D9T18_14160) - 3137676..3138167 (-) 492 WP_121638018.1 Spy/CpxP family protein refolding chaperone -
  D9T18_RS14165 (D9T18_14165) - 3138343..3139032 (+) 690 WP_121638019.1 response regulator -
  D9T18_RS14170 (D9T18_14170) - 3139010..3140425 (+) 1416 WP_054983288.1 ATP-binding protein -
  D9T18_RS14175 (D9T18_14175) - 3140428..3141375 (+) 948 WP_054983287.1 alpha/beta fold hydrolase -
  D9T18_RS14180 (D9T18_14180) - 3141335..3142252 (-) 918 WP_029773365.1 cation diffusion facilitator family transporter -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 61977.14 Da        Isoelectric Point: 5.0828

>NTDB_id=321127 D9T18_RS14150 WP_029773362.1 3135654..3137330(+) (pilB) [Pseudoalteromonas agarivorans strain Hao 2018]
MNINSPLLRKFITLGRIDADTVKAKQQEFASTAELISKCGKIDSNDLAEQCIDLFRVPYFDLKDFDPAQIPEDLVKEKLI
RKHHILPLVQKGRKVYIAASDPTDYGAFENFEFSTGLSCEIVVVDYIQLDNKIEQLLDAAGSLDLSADEFKEFADLDSEG
PKETTPKDDDKDDAPIIVYINKILMDAIKKGASDLHFEPYEHKYRIRFRIDGILHEVANPPNTLASKLSARIKVMSRLDI
AEKRKPQDGRIKLKITERKSIDFRVSTMPTLWGEKIVMRILDSSSAMLGIDVLGYEEDQKKLYMDALGQPQGMILVTGPT
GSGKTVSLYTGLNILNQPERNISTAEDPVEINLEGVNQVQINPKAEMTFANALRAFLRQDPDVVMVGEIRDLETAEISIK
AAQTGHLVLSTLHTNSAPETITRLLNMGVPAYNVASSISLIIAQRLARRLCPKCKTPETLPAEELARQGFSTTQIEDMTL
YAPKGCENCTDGYKGRVGIYEVMQITPEIAQIIMRGGNSLEIAEVSLQAGFNNLRLSGLRKAADGLTSLAEINRVTNM

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=321127 D9T18_RS14150 WP_029773362.1 3135654..3137330(+) (pilB) [Pseudoalteromonas agarivorans strain Hao 2018]
ATGAATATTAACTCACCGTTATTGAGAAAATTTATAACGCTTGGCCGCATTGATGCCGATACCGTAAAAGCAAAACAGCA
AGAGTTTGCCTCAACCGCTGAGCTTATATCTAAATGTGGAAAAATTGACAGCAACGACTTAGCTGAACAATGTATAGACT
TATTTCGCGTACCGTATTTTGATTTAAAAGACTTCGACCCTGCACAAATCCCCGAAGACTTAGTTAAAGAAAAGCTAATA
CGTAAACACCACATTTTACCGTTGGTTCAAAAAGGCAGAAAAGTATACATTGCCGCCTCAGATCCGACTGACTACGGTGC
CTTTGAAAACTTTGAATTTAGCACCGGCTTATCGTGCGAAATAGTCGTGGTTGACTATATTCAGCTAGATAACAAAATTG
AGCAACTACTTGATGCTGCAGGCAGCTTAGATTTAAGCGCCGATGAATTTAAAGAGTTTGCCGACCTAGATAGTGAAGGC
CCTAAAGAAACCACACCTAAAGATGATGATAAAGACGATGCGCCAATCATTGTTTATATCAATAAAATATTAATGGATGC
CATTAAAAAAGGCGCTTCTGATTTACACTTTGAACCTTACGAGCACAAATACCGTATTCGCTTTCGTATTGATGGTATTT
TGCATGAAGTTGCCAACCCACCCAATACCTTAGCAAGTAAGCTATCGGCGCGTATCAAAGTAATGTCGAGGCTCGATATT
GCTGAAAAACGTAAACCACAAGATGGCCGTATTAAATTAAAAATTACCGAGCGTAAAAGCATCGATTTTCGTGTAAGTAC
TATGCCTACGTTATGGGGCGAAAAAATAGTAATGCGTATTCTTGATTCATCAAGCGCCATGCTAGGTATTGACGTATTAG
GTTACGAAGAGGATCAAAAAAAGCTCTACATGGATGCGCTTGGGCAACCGCAAGGCATGATACTAGTTACCGGCCCAACG
GGCTCTGGTAAAACCGTATCGCTTTATACAGGTTTAAATATACTTAACCAGCCAGAGCGTAACATTAGTACCGCCGAGGA
TCCGGTAGAGATAAACCTTGAAGGGGTTAACCAAGTACAAATTAACCCTAAAGCCGAAATGACCTTTGCTAACGCCCTGC
GTGCATTTTTACGTCAAGATCCAGATGTAGTGATGGTAGGTGAAATACGTGACCTAGAAACCGCAGAAATTTCAATTAAA
GCGGCGCAAACAGGTCACTTAGTACTATCAACACTACATACCAACTCAGCGCCAGAAACCATAACGCGTTTATTAAATAT
GGGGGTGCCTGCGTATAACGTGGCAAGCTCTATAAGTTTGATTATTGCCCAGCGCTTAGCTAGGCGTTTATGCCCTAAGT
GTAAAACTCCAGAAACACTGCCAGCAGAAGAATTGGCTCGTCAAGGATTTAGCACAACACAAATTGAAGATATGACACTA
TACGCACCCAAAGGCTGCGAAAATTGCACCGATGGTTACAAAGGCCGTGTGGGTATTTACGAAGTAATGCAAATTACCCC
TGAAATTGCACAAATTATTATGCGCGGTGGCAACTCGCTCGAAATCGCAGAGGTATCGCTACAAGCTGGGTTTAATAACT
TGCGATTATCGGGTTTACGAAAAGCCGCCGATGGTTTAACGTCTCTGGCTGAAATTAATCGCGTAACCAATATGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(182-449)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.81

100

0.568
  pilB Legionella pneumophila strain ERS1305867

53.165

99.104

0.527
  pilB Acinetobacter baylyi ADP1

58.02

90.502

0.525
  pilB Vibrio cholerae strain A1552

52.799

96.057

0.507
  pilB Vibrio parahaemolyticus RIMD 2210633

52.82

95.341

0.504
  pilB Vibrio campbellii strain DS40M4

51.88

95.341

0.495
  pilF Neisseria gonorrhoeae MS11

49.042

93.548

0.459
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.645

90.86

0.36

Multiple sequence alignment