Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   FEF05_RS03960 Genome accession   NZ_CP040243
Coordinates   766715..767977 (+) Length   420 a.a.
NCBI ID   WP_026917118.1    Uniprot ID   -
Organism   Glaesserella parasuis strain HPS-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 761715..772977
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FEF05_RS03935 (FEF05_03940) - 761909..764464 (-) 2556 WP_035490200.1 penicillin-binding protein 1A -
  FEF05_RS03940 (FEF05_03945) comA 764618..765295 (+) 678 WP_005712510.1 hypothetical protein Machinery gene
  FEF05_RS03945 (FEF05_03950) comB 765271..765792 (+) 522 WP_005712507.1 hypothetical protein Machinery gene
  FEF05_RS03950 (FEF05_03955) comC 765789..766319 (+) 531 WP_149350765.1 ATPase Machinery gene
  FEF05_RS03955 (FEF05_03960) comD 766319..766705 (+) 387 WP_149350766.1 pilus assembly protein PilP Machinery gene
  FEF05_RS03960 (FEF05_03965) comE 766715..767977 (+) 1263 WP_026917118.1 type IV pilus secretin PilQ Machinery gene
  FEF05_RS03965 (FEF05_03970) nusB 768056..768469 (+) 414 WP_005712500.1 transcription antitermination factor NusB -
  FEF05_RS03970 (FEF05_03975) thiL 768478..769446 (+) 969 WP_021110497.1 thiamine-phosphate kinase -
  FEF05_RS03975 (FEF05_03980) - 769449..769919 (+) 471 WP_005712497.1 phosphatidylglycerophosphatase A -
  FEF05_RS03980 (FEF05_03985) - 769921..770550 (+) 630 WP_010785950.1 LysE family transporter -
  FEF05_RS03985 (FEF05_03990) trxA 770847..771161 (+) 315 WP_149350767.1 thioredoxin -
  FEF05_RS12100 - 771706..771993 (+) 288 WP_016528248.1 hypothetical protein -
  FEF05_RS03995 (FEF05_04000) - 772545..772964 (-) 420 WP_005714137.1 hotdog fold thioesterase -

Sequence

Protein


Download         Length: 420 a.a.        Molecular weight: 47095.07 Da        Isoelectric Point: 8.9232

>NTDB_id=320686 FEF05_RS03960 WP_026917118.1 766715..767977(+) (comE) [Glaesserella parasuis strain HPS-1]
MRYLFLLFFATFPVLANQQISLSIRNAPTAEIISYLAEETGKNITISDEIKDTKNFRVEKSHFDEILNSLIKTHQLNLKK
ENGIYYIHQAQEHKQHTTAQLVNALPKLITKTIKLHYSKASEVIESLTKGQGNLLSENGYLHFDDRSNSIIVKDSAASVK
NFTQLIESLDKPTEQIAIEARIVTISSEHLQQLGVRWGLFSPNENHYKLAGNLEGNGLTTNNLNVNFPVNPSASVALQIA
AINSRVLHLELTALESENNIEIIASPRLLTTDKKPASIKQGTEIPYAMYSKKKEITDIEFREAVLGLEVTPHISKQNQIL
LDLAISQNSPNNQMNNMMATIDKQEINTQVLAKHGETIVLGGIFQHLIAKGEDKVPLLGSIPVIKRLFSQNRDKISKREL
VIFVTPYIVKSEKMGAEKQK

Nucleotide


Download         Length: 1263 bp        

>NTDB_id=320686 FEF05_RS03960 WP_026917118.1 766715..767977(+) (comE) [Glaesserella parasuis strain HPS-1]
ATGCGTTATTTATTCCTGCTATTCTTTGCTACTTTTCCTGTATTAGCAAACCAACAAATTTCACTTTCGATAAGAAATGC
CCCAACGGCAGAGATTATTAGTTATTTGGCGGAGGAAACTGGAAAGAATATTACAATTTCGGATGAAATTAAAGATACAA
AAAATTTCAGAGTAGAAAAAAGCCATTTTGATGAGATATTAAATAGTTTAATCAAAACACATCAATTAAATTTAAAAAAA
GAAAATGGCATTTACTATATTCATCAAGCCCAAGAACATAAACAACATACTACGGCACAATTAGTTAATGCTCTGCCTAA
ATTAATCACCAAAACAATCAAGTTACACTATTCCAAAGCCTCTGAAGTAATAGAGTCTTTGACAAAAGGGCAAGGCAACT
TGCTATCAGAGAATGGTTATCTTCATTTTGATGATCGCAGTAATAGTATTATCGTCAAAGACAGTGCCGCCTCAGTTAAA
AACTTTACTCAACTTATCGAAAGCCTAGATAAACCAACGGAACAAATTGCAATTGAAGCCCGAATTGTCACGATCAGCAG
TGAACATTTACAACAACTTGGCGTGCGTTGGGGCTTATTTTCCCCTAACGAAAATCACTACAAATTGGCAGGAAACTTAG
AGGGCAACGGATTAACTACCAACAACTTAAACGTAAATTTTCCAGTAAATCCGTCCGCTTCCGTTGCCTTACAAATTGCC
GCTATCAACAGCCGTGTACTTCATTTAGAACTCACCGCATTAGAGAGCGAAAATAACATTGAGATCATTGCAAGCCCTCG
CTTACTCACAACAGATAAAAAACCAGCGAGTATCAAACAAGGTACAGAGATTCCTTATGCAATGTACAGTAAGAAAAAGG
AAATCACCGATATTGAATTTCGTGAAGCGGTTTTGGGGCTAGAAGTCACGCCACATATTTCTAAACAAAATCAGATTTTG
TTAGATCTTGCCATTAGCCAAAATTCGCCAAATAACCAGATGAACAATATGATGGCAACGATTGATAAACAAGAAATTAA
TACACAAGTCCTTGCTAAACACGGCGAAACCATCGTATTAGGCGGTATTTTTCAACATCTGATCGCCAAAGGCGAAGATA
AAGTCCCGCTGTTAGGCAGTATCCCCGTGATTAAACGCTTATTTAGCCAAAACCGAGATAAAATCTCTAAACGGGAGCTG
GTTATTTTTGTTACGCCTTATATTGTAAAATCTGAAAAAATGGGAGCGGAAAAGCAGAAATAA

Domains

Predicted by InterProScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(110-175)

Bacterial type II and III secretion system protein (Secretin)

(253-410)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

97.381

100

0.974
  comE Haemophilus influenzae Rd KW20

52.706

100

0.533
  comE Haemophilus influenzae 86-028NP

51.765

100

0.524
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.681

98.571

0.371
  pilQ Vibrio cholerae strain A1552

37.681

98.571

0.371
  pilQ Vibrio campbellii strain DS40M4

36.493

100

0.367