Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   SWP_RS21140 Genome accession   NC_011566
Coordinates   4990025..4991731 (+) Length   568 a.a.
NCBI ID   WP_020914722.1    Uniprot ID   B8CUR6
Organism   Shewanella piezotolerans WP3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4985025..4996731
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SWP_RS21120 (swp_4754) ampE 4985192..4986043 (-) 852 WP_020914715.1 beta-lactamase regulator AmpE -
  SWP_RS21125 (swp_4755) ampD 4986090..4986632 (-) 543 WP_020914716.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  SWP_RS21130 (swp_4756) nadC 4987118..4988002 (+) 885 WP_020914717.1 carboxylating nicotinate-nucleotide diphosphorylase -
  SWP_RS24855 - 4989515..4989614 (+) 100 Protein_4154 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  SWP_RS21140 (swp_4761) pilB 4990025..4991731 (+) 1707 WP_020914722.1 type IV-A pilus assembly ATPase PilB Machinery gene
  SWP_RS21145 (swp_4762) pilC 4991776..4993041 (+) 1266 WP_020914723.1 type II secretion system F family protein Machinery gene
  SWP_RS21150 (swp_4763) pilD 4993095..4994006 (+) 912 WP_020914724.1 prepilin peptidase Machinery gene
  SWP_RS21155 (swp_4764) coaE 4994236..4994841 (+) 606 WP_020914725.1 dephospho-CoA kinase -
  SWP_RS21160 (swp_4765) zapD 4994850..4995584 (+) 735 WP_020914726.1 cell division protein ZapD -
  SWP_RS21165 (swp_4766) yacG 4995633..4995869 (+) 237 WP_020914727.1 DNA gyrase inhibitor YacG -
  SWP_RS21170 (swp_4767) mutT 4995866..4996261 (+) 396 WP_020914728.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 63001.25 Da        Isoelectric Point: 5.3436

>NTDB_id=31813 SWP_RS21140 WP_020914722.1 4990025..4991731(+) (pilB) [Shewanella piezotolerans WP3]
MPTTGLHLGLSTLFIRKNLLSEEQISSAISISRKKHTSLVSTIIQQQLISARTIAELCYEEYGTPLLDLNEFDVAAIPDE
FLNRKLIEKHKCLPLFKRGNRLYIATSDPTNIAALEDFQFSAGLHAEAILVEDDKLSKALESVLEEDITSLDLSAIDEEA
LAGIEVTDTDKRDEEQGDGKDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILQEVSEPPVNLSGRISAR
LKVMSKLDIAERRVPQDGRIKMKMSRTKSIDFRVSTLPTIWGEKIVMRILDSSSAQLGIEKLGYEDDQRLMYEEMLAKPQ
GMILVTGPTGSGKTVSLYTGLNILNTAERNISTAEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLSRRLCQHCKTPEEIPTDELLKLGFS
QTQIDTDITPYKPVGCEHCTAGYKGRVGIYEVMKMSDEIARTIMEGGNSLQILKQAKSQGMRDLRDSGLLKVTQGVTSIA
EINRVTSF

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=31813 SWP_RS21140 WP_020914722.1 4990025..4991731(+) (pilB) [Shewanella piezotolerans WP3]
ATGCCTACCACAGGTCTTCATCTTGGCTTATCTACTCTTTTTATTCGCAAAAACCTACTTAGCGAAGAGCAAATATCATC
AGCTATATCTATTTCTCGTAAAAAGCATACCTCGCTAGTCAGCACCATTATCCAGCAGCAGTTGATATCAGCTCGAACCA
TTGCCGAACTCTGCTATGAAGAATATGGTACTCCTCTACTCGATCTTAATGAGTTTGATGTTGCAGCCATTCCCGATGAA
TTTCTCAATAGAAAACTGATTGAGAAGCACAAATGTTTACCGTTATTTAAGCGTGGTAACCGCCTCTATATCGCCACCTC
TGATCCCACTAATATCGCCGCTTTAGAGGATTTTCAATTCAGCGCGGGTTTGCACGCCGAAGCTATTCTAGTTGAAGATG
ATAAGCTCAGCAAAGCACTAGAGAGTGTACTTGAAGAGGATATTACATCACTTGACTTAAGCGCTATTGATGAAGAAGCC
CTTGCAGGCATCGAAGTTACCGATACCGATAAACGCGATGAAGAACAAGGTGATGGAAAAGATGACGCGCCTATCGTCAT
TTACATCAATAAAATTCTTACTGATGCCATTCGCAAGGGTGCATCAGATCTGCACTTTGAGCCTTATGAGAAACGCTACC
GCATTCGTTTTCGTATCGACGGTATCTTGCAAGAGGTTTCAGAGCCACCAGTCAACCTTTCTGGCCGTATTTCAGCCCGC
TTAAAGGTAATGTCCAAACTTGATATAGCTGAGCGCCGAGTGCCGCAAGATGGTCGCATCAAGATGAAGATGTCGCGCAC
TAAATCTATCGATTTTCGTGTCAGCACTCTGCCCACTATTTGGGGTGAAAAAATAGTGATGCGTATTCTGGACTCCTCTT
CGGCGCAGTTAGGTATCGAAAAGCTGGGTTATGAAGATGACCAAAGGCTGATGTACGAGGAGATGCTGGCCAAACCCCAA
GGGATGATATTGGTTACTGGCCCTACCGGTTCTGGTAAAACAGTATCTCTCTATACAGGGTTAAATATCCTTAACACTGC
TGAGCGCAATATCTCTACCGCCGAAGATCCGGTGGAGATCAACCTTGAAGGCGTTAACCAAGTTCATATTAACCTTAAAG
CGGGGTTAACTTTTGCCTCTGCGCTGCGCTCATTCTTGCGTCAAGATCCTGATGTGGTGATGGTCGGTGAAATTCGAGAC
TTAGAAACCGCTGAGATTGCCATTAAGGCTGCGCAAACCGGTCATTTAGTACTATCGACCTTGCATACCAACTCCGCTGC
TGAGACTTTAACACGTCTAATCAATATGGGCGTTCCAGGCTATAACATTGCCAGCTCGGTTAACCTAATTATTGCTCAGC
GGCTATCAAGGCGCCTATGCCAACACTGTAAAACCCCTGAAGAGATCCCAACGGATGAACTACTGAAGCTTGGCTTTAGC
CAAACACAAATAGATACAGATATTACCCCCTATAAACCAGTCGGCTGTGAACACTGCACTGCCGGTTATAAAGGCCGTGT
CGGCATCTATGAAGTGATGAAGATGTCAGATGAGATCGCCCGCACCATTATGGAGGGAGGGAATTCATTGCAAATTCTTA
AGCAGGCTAAATCGCAAGGGATGCGAGATCTGAGGGATTCAGGTCTGCTAAAGGTTACTCAAGGTGTAACTAGTATTGCC
GAGATTAATCGTGTTACCAGCTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B8CUR6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.289

98.768

0.576
  pilB Acinetobacter baumannii D1279779

57.016

99.12

0.565
  pilB Legionella pneumophila strain ERS1305867

56.406

98.944

0.558
  pilB Vibrio parahaemolyticus RIMD 2210633

51.681

99.472

0.514
  pilB Vibrio cholerae strain A1552

51.515

98.768

0.509
  pilB Vibrio campbellii strain DS40M4

50.267

98.768

0.496
  pilF Neisseria gonorrhoeae MS11

47.257

99.472

0.47
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.448

97.535

0.375
  pilF Thermus thermophilus HB27

37.123

99.12

0.368

Multiple sequence alignment