Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   CN228_RS29095 Genome accession   NZ_CP032631
Coordinates   6015250..6017355 (-) Length   701 a.a.
NCBI ID   WP_017682642.1    Uniprot ID   A0AAN4TP60
Organism   Pseudomonas syringae pv. actinidiae str. Shaanxi_M228     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6010250..6022355
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CN228_RS29080 (CN228_29080) - 6011875..6013494 (-) 1620 WP_003378845.1 AAA family ATPase -
  CN228_RS29085 (CN228_29085) aroB 6013505..6014608 (-) 1104 WP_003378846.1 3-dehydroquinate synthase -
  CN228_RS29090 (CN228_29090) aroK 6014727..6015245 (-) 519 WP_003378847.1 shikimate kinase AroK -
  CN228_RS29095 (CN228_29095) pilQ 6015250..6017355 (-) 2106 WP_017682642.1 type IV pilus secretin PilQ Machinery gene
  CN228_RS29100 (CN228_29100) pilP 6017403..6017930 (-) 528 WP_003378849.1 type 4a pilus biogenesis lipoprotein PilP -
  CN228_RS29105 (CN228_29105) pilO 6017927..6018550 (-) 624 WP_003378850.1 type 4a pilus biogenesis protein PilO -
  CN228_RS29110 (CN228_29110) - 6018547..6019173 (-) 627 WP_003378851.1 PilN domain-containing protein -
  CN228_RS29115 (CN228_29115) pilM 6019173..6020237 (-) 1065 WP_003378852.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 701 a.a.        Molecular weight: 75416.87 Da        Isoelectric Point: 4.9774

>NTDB_id=317366 CN228_RS29095 WP_017682642.1 6015250..6017355(-) (pilQ) [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228]
MTRILSIIGVSLCIAMLSPVLQAANLKTLDVAALPGDRIELKLAFDAPVPAPRGYTTAQPARIALDLPGVTSQLETKSRE
LGTGNARSVVVVQAQDRTRVIINLTTLAPYSSRVDGNNLFVVIGQGAAAAQASQPTTAMGAQRVAVPPPVPSSAKPFIPA
GVKAIRNVDFQRGELGEGNVVIELSNPSIAPDIQEQGGKIRVDFAKTQLPEPLRVRLDVKDFATPVQFVSASATGDKASI
LIEPSGAFDYSAFQTENKLTISVRPLTNEDLDRRAADKPVYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGI
TLRLQNVPWDQALDLVLKTKGLDKRKVGSVLLVAPADEIAARERQELESLKQIAELAPLRRELLQVNYAKAADIAKLFQS
VTSAESKAEERGSITVDDRTNNIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEANVDYNKQLGVRWGGSTNTSGSG
KWTTYGLDDNGDEAGNTSGNLTSNVPFVDLGAAGATSGIGLGFVTNNTLLDLELSAMEKTGNGEIVSQPKVVTSDKETAK
ILKGTEIPYQESSSSGATTVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNAVLGVPPIKKNEVNAKVLISDGET
IVIGGVFSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSESKSELLVFLTPRIMNNQAISVSR

Nucleotide


Download         Length: 2106 bp        

>NTDB_id=317366 CN228_RS29095 WP_017682642.1 6015250..6017355(-) (pilQ) [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228]
ATGACCCGGATTCTCTCGATTATCGGCGTGTCGTTATGCATAGCGATGCTGTCGCCGGTTCTCCAGGCTGCCAACCTCAA
GACCCTGGATGTCGCCGCCCTGCCAGGCGATCGCATCGAGCTCAAGCTCGCCTTTGACGCGCCGGTGCCCGCGCCTCGCG
GCTACACCACCGCGCAGCCGGCGCGCATCGCTCTGGACCTGCCGGGCGTGACCAGCCAGCTGGAGACCAAAAGCCGTGAG
CTGGGCACCGGCAATGCACGCAGCGTAGTGGTGGTGCAGGCTCAGGATCGCACGCGAGTGATCATCAACCTGACGACGCT
TGCGCCTTACAGCTCCCGCGTCGACGGCAACAATCTGTTCGTGGTCATCGGCCAGGGTGCCGCCGCAGCGCAGGCTTCCC
AGCCGACCACTGCGATGGGCGCGCAACGGGTAGCAGTGCCGCCGCCTGTCCCGTCGTCTGCCAAGCCCTTTATACCGGCG
GGCGTCAAAGCCATCCGCAATGTCGATTTTCAGCGCGGTGAGCTGGGTGAAGGCAATGTGGTCATCGAACTGAGTAACCC
GAGCATCGCGCCTGATATTCAGGAGCAGGGCGGCAAGATCCGTGTCGACTTCGCCAAGACCCAGTTGCCCGAGCCTCTGC
GTGTGCGGCTGGACGTCAAGGATTTCGCGACCCCTGTGCAGTTCGTCAGTGCGTCGGCCACGGGCGACAAGGCCAGTATT
CTGATCGAGCCGTCGGGTGCCTTCGATTACTCTGCGTTCCAGACCGAAAACAAGCTGACCATCAGCGTACGTCCGTTGAC
CAACGAAGATCTGGACCGCCGCGCCGCCGACAAGCCGGTGTACACCGGTGAAAAGCTCTCGCTTAACTTCCAGGACATCG
ACGTGCGTTCGGTGCTGCAACTGATCGCCGACTTCACCAACCTCAATCTGGTGGCCAGTGACACGGTTCAGGGCGGCATC
ACCCTGCGCCTGCAGAACGTGCCGTGGGATCAGGCGCTGGACCTGGTGCTGAAAACCAAAGGCCTGGACAAGCGCAAGGT
CGGCAGTGTTCTGCTGGTTGCGCCAGCGGATGAAATTGCCGCCCGCGAACGCCAGGAGCTGGAATCGCTCAAGCAGATTG
CCGAACTGGCGCCGCTGCGCCGCGAGCTGCTGCAGGTCAATTACGCCAAGGCCGCCGACATCGCCAAGCTGTTCCAGTCG
GTGACCAGTGCCGAGTCCAAGGCTGAAGAGCGGGGCTCGATCACCGTGGACGACCGCACCAACAACATCATTGCCTACCA
GACTCAGGACCGTCTCGACGAACTGCGTCGCATCGTTTCGCAACTGGACATCCCGGTGCGCCAGGTGATGATCGAGGCAC
GCATCGTCGAGGCCAACGTCGATTACAACAAGCAGTTGGGCGTGCGCTGGGGCGGTTCGACCAACACCAGCGGCAGCGGG
AAATGGACGACTTACGGGCTGGATGACAATGGCGATGAGGCCGGTAACACGAGCGGCAATCTGACTTCCAACGTTCCGTT
CGTGGACCTGGGGGCCGCCGGGGCGACGTCCGGCATCGGTCTGGGCTTTGTGACCAATAACACCTTGCTGGACCTTGAGC
TGAGCGCGATGGAGAAGACCGGCAACGGCGAAATCGTCTCCCAGCCCAAAGTGGTCACGTCCGACAAGGAAACCGCCAAG
ATCCTCAAGGGCACGGAGATTCCGTATCAGGAATCCAGCTCCAGCGGCGCCACCACCGTAAGCTTCAAGGAGGCTTCGCT
GTCGCTTGAAGTGACGCCGCAGATCACGCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACT
ACCTGAATGCGGTGCTTGGCGTACCGCCGATCAAGAAAAACGAGGTCAACGCCAAAGTGCTGATTTCCGATGGCGAAACC
ATCGTGATCGGCGGTGTGTTCTCCAATACGCAAAGTAAAGTTGTCGATAAAGTGCCATTTCTTGGCGATGTACCGTATCT
TGGCCGGCTTTTCAGGCGCGATGTGGTATCCGAGTCCAAATCCGAGCTGTTGGTATTTTTGACTCCGCGTATCATGAACA
ACCAGGCGATTTCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(383-448)

AMIN domain (AMIN)

(38-122)

Secretin and TonB N terminus short domain (STN)

(309-355)

Bacterial type II and III secretion system protein (Secretin)

(536-692)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.476

100

0.748
  comQ Acinetobacter baylyi ADP1

41.774

99.715

0.417
  pilQ Acinetobacter baumannii strain A118

38.639

100

0.405
  pilQ Acinetobacter baumannii D1279779

38.639

100

0.405
  pilQ Legionella pneumophila strain Lp02

38.696

98.431

0.381
  pilQ Legionella pneumophila strain ERS1305867

38.507

97.432

0.375

Multiple sequence alignment