Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   D6869_RS11930 Genome accession   NZ_CP032526
Coordinates   2344682..2345128 (-) Length   148 a.a.
NCBI ID   WP_011836043.1    Uniprot ID   A0A1V0PET3
Organism   Lactococcus cremoris strain 4B0     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 2339816..2353781 2344682..2345128 within 0


Gene organization within MGE regions


Location: 2339816..2353781
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D6869_RS11900 (D6869_12055) - 2339816..2341211 (-) 1396 Protein_2334 IS3 family transposase -
  D6869_RS11905 (D6869_12060) - 2341284..2342093 (-) 810 WP_011836038.1 metal ABC transporter permease -
  D6869_RS11910 (D6869_12065) - 2342086..2342823 (-) 738 WP_011836039.1 metal ABC transporter ATP-binding protein -
  D6869_RS11915 (D6869_12070) - 2343002..2343844 (-) 843 WP_011836040.1 zinc ABC transporter substrate-binding protein -
  D6869_RS11920 (D6869_12075) - 2343841..2344278 (-) 438 WP_011836041.1 zinc-dependent MarR family transcriptional regulator -
  D6869_RS11925 (D6869_12080) comGG 2344359..2344658 (-) 300 WP_011836042.1 competence type IV pilus minor pilin ComGG Machinery gene
  D6869_RS11930 (D6869_12085) comGF 2344682..2345128 (-) 447 WP_011836043.1 competence type IV pilus minor pilin ComGF Machinery gene
  D6869_RS11935 (D6869_12090) comGE 2345091..2345387 (-) 297 WP_014735175.1 competence type IV pilus minor pilin ComGE Machinery gene
  D6869_RS11940 (D6869_12095) comGD 2345359..2345802 (-) 444 WP_011836045.1 competence type IV pilus minor pilin ComGD Machinery gene
  D6869_RS11945 (D6869_12100) comGC 2345750..2346100 (-) 351 WP_042211582.1 competence type IV pilus major pilin ComGC Machinery gene
  D6869_RS11950 (D6869_12105) comGB 2346145..2347170 (-) 1026 WP_042211510.1 competence type IV pilus assembly protein ComGB Machinery gene
  D6869_RS11955 (D6869_12110) comGA 2347070..2348050 (-) 981 WP_011836048.1 competence type IV pilus ATPase ComGA Machinery gene
  D6869_RS11960 (D6869_12115) - 2348164..2351958 (-) 3795 Protein_2346 PolC-type DNA polymerase III -
  D6869_RS13225 - 2352478..2353071 (-) 594 Protein_2347 PolC-type DNA polymerase III N-terminal domain-containing protein -
  D6869_RS11965 (D6869_12120) - 2353245..2353781 (-) 537 WP_011836050.1 hypothetical protein -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 17102.82 Da        Isoelectric Point: 8.2376

>NTDB_id=316732 D6869_RS11930 WP_011836043.1 2344682..2345128(-) (comGF) [Lactococcus cremoris strain 4B0]
MERKFCDLKLKIRAFTLLECLVALLAISGSVLVISGLTRMIEEQMKISQNDSRKDWQIFCEQMRSELSGAKLDNVNQNFL
YVTKDKKLRFGLVGDDFRKSDDKGQGYQPMLYDLKGAKIQAEENLIKITIDFDNGGERVFIYRFTDTK

Nucleotide


Download         Length: 447 bp        

>NTDB_id=316732 D6869_RS11930 WP_011836043.1 2344682..2345128(-) (comGF) [Lactococcus cremoris strain 4B0]
ATGGAAAGGAAATTTTGCGACTTGAAGCTCAAAATTAGGGCATTTACTTTACTAGAATGTCTCGTAGCACTTTTGGCAAT
TTCTGGTTCAGTGCTAGTCATCTCAGGGCTGACTAGAATGATTGAAGAACAGATGAAAATAAGCCAGAATGATAGTAGGA
AAGATTGGCAAATATTTTGTGAACAAATGCGTTCAGAACTCTCCGGAGCAAAATTAGACAATGTGAATCAAAATTTTTTG
TATGTGACCAAAGATAAGAAGTTACGGTTCGGATTAGTAGGGGATGATTTTCGTAAAAGTGATGATAAAGGGCAAGGATA
CCAACCGATGCTTTATGATTTAAAAGGAGCAAAAATTCAGGCAGAAGAAAATTTGATAAAAATAACAATTGATTTTGATA
ATGGAGGTGAGCGAGTATTTATTTATCGATTTACAGATACAAAGTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(45-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Lactococcus lactis subsp. cremoris KW2

99.291

95.27

0.946
  comYF Streptococcus mutans UA140

44.681

95.27

0.426
  comYF Streptococcus mutans UA159

43.972

95.27

0.419
  comGF/cglF Streptococcus mitis SK321

39.286

94.595

0.372
  comGF/cglF Streptococcus mitis NCTC 12261

39.286

94.595

0.372

Multiple sequence alignment