Detailed information NTG0316715

Bioinformatically predicted

Overview

• Name: dprA
• Type: Machinery gene
• Locus tag: D6869_RS06405
• Genome accession: NZ_CP032526
• Coordinates: 1244075..1244923 (-)
• Length: 282 a.a.
• NCBI ID: WP_011835152.1
• Uniprot ID: Q25AZ2
• Organism: Lactococcus cremoris strain 4B0
• Function: ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 1239075..1249923

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D6869_RS06390 (D6869_06485)	xerS	1239145..1240215 (+)	1071	WP_011835149.1	tyrosine recombinase XerS	-
D6869_RS06395 (D6869_06490)	trmFO	1240265..1241611 (-)	1347	WP_011835150.1	methylenetetrahydrofolate--tRNA-(uracil(54)- C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO	-
D6869_RS06400 (D6869_06495)	topA	1241683..1243809 (-)	2127	WP_011835151.1	type I DNA topoisomerase	-
D6869_RS06405 (D6869_06500)	dprA	1244075..1244923 (-)	849	WP_011835152.1	DNA-processing protein DprA	Machinery gene
D6869_RS06410 (D6869_06505)	-	1245071..1245451 (-)	381	WP_011835153.1	RidA family protein	-
D6869_RS06415 (D6869_06510)	budA	1245491..1246201 (-)	711	WP_011835154.1	acetolactate decarboxylase	-
D6869_RS06420 (D6869_06515)	ilvA	1246337..1247587 (-)	1251	WP_011835155.1	threonine ammonia-lyase IlvA	-
D6869_RS06425 (D6869_06520)	ilvC	1247646..1248668 (-)	1023	WP_011835156.1	ketol-acid reductoisomerase	-
D6869_RS06430 (D6869_06525)	ilvN	1248713..1249189 (-)	477	WP_011835157.1	acetolactate synthase small subunit	-

Sequence

Protein

• Length: 282 a.a.
• Molecular weight: 31919.06 Da
• Isoelectric Point: 9.9168

>NTDB_id=316715 D6869_RS06405 WP_011835152.1 1244075..1244923(-) (dprA) [Lactococcus cremoris strain 4B0]
MITNFDLFRWKKAGMINLGVNKLLKFFHKYDRKISLRQMGQVAQVKSIPNFIEQYKNQDVKKLRIDYKKFSSFSILDDLY
PERLREIYNPPVLIFYQGNIDLLKNPKLAFVGSRLAGQSGIKAVQKIVTELNQSFTIVSGLAKGIDTASHLSAIKTKTPT
IAVIGTGLDIFYPLENRKIQEYLAKNQLVLSEYSLGEKPLKYHFPERNRIIAGLSRGVVVVEAKLRSGSLITCERALEEG
RDIFAIPGNIADGTSDGCNHLIQQGAKLVYQAQDILEEYLYN

Nucleotide

• Length: 849 bp

>NTDB_id=316715 D6869_RS06405 WP_011835152.1 1244075..1244923(-) (dprA) [Lactococcus cremoris strain 4B0]
ATGATTACAAATTTTGATTTGTTTCGTTGGAAAAAAGCAGGAATGATCAATCTTGGTGTAAATAAGCTCTTAAAATTTTT
CCACAAATATGATAGAAAAATAAGCTTACGACAAATGGGACAAGTGGCTCAAGTCAAATCAATCCCTAACTTTATCGAAC
AATATAAAAATCAGGACGTAAAGAAACTCAGGATAGACTATAAAAAATTTTCCTCCTTTTCCATTCTTGATGACCTTTAT
CCCGAGCGTCTAAGAGAAATCTATAACCCACCCGTCTTAATTTTTTATCAGGGAAATATTGATTTATTAAAAAACCCTAA
ACTTGCCTTTGTTGGAAGCCGCCTAGCAGGTCAATCAGGGATAAAAGCTGTCCAAAAAATTGTGACAGAATTGAATCAAT
CCTTTACAATAGTAAGCGGACTTGCCAAAGGGATTGACACAGCCAGCCATCTGTCAGCAATTAAAACTAAAACACCAACA
ATTGCTGTTATTGGTACAGGATTGGATATTTTCTATCCACTAGAAAATCGTAAAATACAGGAATATCTAGCAAAAAATCA
ATTAGTTTTAAGTGAATATTCATTAGGCGAAAAACCACTTAAATACCACTTCCCAGAGCGCAATCGAATTATTGCTGGTC
TTTCACGGGGAGTTGTTGTCGTGGAAGCAAAATTAAGAAGTGGAAGTTTAATTACCTGTGAAAGGGCACTGGAAGAAGGA
AGAGATATCTTTGCCATCCCTGGAAATATTGCAGATGGAACATCTGATGGCTGTAATCATCTTATTCAACAAGGAGCAAA
ACTTGTTTATCAAGCTCAAGATATTTTAGAAGAATATTTGTACAATTAA

Domains

Predicted by InterproScan.

• DNA recombination-mediator protein A (DNA_processg_A) (69-270)

• DNA processing protein A sterile alpha motif domain (SAM_DprA) (2-63)

3D structure

• AlphaFold DB: Q25AZ2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
dprA	Lactococcus lactis subsp. cremoris KW2	98.227	100	0.982
dprA	Streptococcus mutans UA159	58.633	98.582	0.578
dprA/cilB/dalA	Streptococcus mitis SK321	55.357	99.291	0.55
dprA/cilB/dalA	Streptococcus pneumoniae R6	55	99.291	0.546
dprA/cilB/dalA	Streptococcus mitis NCTC 12261	55	99.291	0.546
dprA/cilB/dalA	Streptococcus pneumoniae TIGR4	55	99.291	0.546
dprA/cilB/dalA	Streptococcus pneumoniae Rx1	55	99.291	0.546
dprA/cilB/dalA	Streptococcus pneumoniae D39	55	99.291	0.546
dprA	Legionella pneumophila strain ERS1305867	39.13	97.872	0.383
dprA	Haemophilus influenzae Rd KW20	39.925	95.035	0.379