Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   D6869_RS07420 Genome accession   NZ_CP032526
Coordinates   1457934..1459226 (+) Length   430 a.a.
NCBI ID   WP_011835332.1    Uniprot ID   A2RL94
Organism   Lactococcus cremoris strain 4B0     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1452934..1464226
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D6869_RS07395 (D6869_07500) glyQ 1453556..1454509 (-) 954 WP_011835327.1 glycine--tRNA ligase subunit alpha -
  D6869_RS07400 (D6869_07505) - 1454738..1455505 (-) 768 WP_011835328.1 5'-nucleotidase -
  D6869_RS07405 (D6869_07510) - 1455615..1456634 (-) 1020 WP_011835329.1 DUF475 domain-containing protein -
  D6869_RS07410 (D6869_07515) - 1456700..1457200 (-) 501 WP_011835330.1 VanZ family protein -
  D6869_RS07415 (D6869_07520) - 1457242..1457877 (-) 636 WP_011835331.1 YigZ family protein -
  D6869_RS07420 (D6869_07525) comFA 1457934..1459226 (+) 1293 WP_011835332.1 DEAD/DEAH box helicase Machinery gene
  D6869_RS12995 comFC 1459472..1459876 (+) 405 WP_242700771.1 phosphoribosyltransferase family protein Machinery gene
  D6869_RS07430 (D6869_07535) - 1459935..1460513 (-) 579 WP_011835334.1 nucleotidyltransferase family protein -
  D6869_RS07435 (D6869_07540) - 1460530..1460985 (-) 456 WP_011676094.1 diacylglycerol kinase -
  D6869_RS07440 (D6869_07545) ybeY 1460969..1461457 (-) 489 WP_011835335.1 rRNA maturation RNase YbeY -
  D6869_RS07445 (D6869_07550) - 1461687..1462175 (-) 489 WP_011835336.1 NUDIX hydrolase -
  D6869_RS07450 (D6869_07555) - 1462245..1463198 (-) 954 WP_041931308.1 PhoH family protein -

Sequence

Protein


Download         Length: 430 a.a.        Molecular weight: 49461.65 Da        Isoelectric Point: 9.9193

>NTDB_id=316717 D6869_RS07420 WP_011835332.1 1457934..1459226(+) (comFA) [Lactococcus cremoris strain 4B0]
MNLILENLLGRLLLEKDISAFYNFTDQVNNENKLIKICAMTSVSANKVRCNRCGTIHIKTNVKLPIGAFFCPTCLELGRV
RSDEYFYHLPQQDFPEKTYLRWTGKLTENQEKISNALCQQITNNQKLLVQAVTGAGKTEMIYQLIEQILSHGGSVGLASP
RIDVCIELHQRLSRDFTCQIPLLYHEGDSYFRSPLVVMTSHQLLRFKEAFDLLIIDEVDAFPFRDNDMLYFALENAKKIN
GNLLYLTATSTDKLDKQIKKHEIKRLFLPRRFHGHPLVIPMFFWKKTFYKKFIEQRKTGFPLLIFVAEIDFGQDFAKNLQ
EKFQKERMAFVASTTKSRKTIVEAFRKKQVSILITTSILERGVTFSSIDVFVINSEHPNFTKSALIQMVGRVGRDSKRPT
GLVSFFHSGKSLAMCQAQKEIKKMNQLGGF

Nucleotide


Download         Length: 1293 bp        

>NTDB_id=316717 D6869_RS07420 WP_011835332.1 1457934..1459226(+) (comFA) [Lactococcus cremoris strain 4B0]
ATGAACTTAATTTTAGAAAATCTCCTTGGGCGTCTTTTACTAGAAAAAGATATTTCAGCTTTTTATAATTTTACTGACCA
AGTTAACAATGAAAATAAATTAATTAAAATTTGTGCAATGACATCTGTCAGTGCTAATAAAGTAAGATGTAATCGATGTG
GCACGATACATATAAAAACCAATGTTAAACTTCCGATTGGTGCTTTTTTCTGTCCAACTTGTCTTGAACTTGGTCGTGTC
CGCTCAGATGAATATTTCTACCATCTTCCTCAACAAGACTTTCCAGAAAAAACTTATTTACGTTGGACTGGAAAATTAAC
AGAAAATCAAGAAAAAATTTCTAACGCTCTCTGTCAGCAAATTACTAATAATCAAAAACTACTTGTTCAAGCCGTTACTG
GTGCTGGTAAAACAGAAATGATATATCAGCTTATTGAACAAATTCTTAGCCATGGGGGTTCGGTCGGTTTAGCCAGCCCA
AGAATTGATGTATGCATTGAGCTTCATCAACGTTTATCACGTGATTTCACTTGTCAAATTCCCCTTTTGTATCATGAGGG
AGATTCTTATTTTCGTTCGCCTTTGGTCGTTATGACCAGTCACCAACTTTTACGTTTCAAAGAGGCCTTTGATTTGCTTA
TCATCGATGAAGTCGATGCCTTTCCTTTTAGAGATAATGACATGCTTTATTTCGCTCTTGAAAATGCTAAAAAAATTAAT
GGAAACTTGCTCTACTTAACCGCTACATCTACTGACAAATTAGACAAACAGATAAAAAAGCATGAAATTAAACGACTTTT
TTTACCACGCCGCTTTCACGGTCATCCTTTGGTCATTCCAATGTTTTTTTGGAAAAAAACATTTTATAAAAAATTCATTG
AGCAAAGAAAGACTGGTTTCCCACTTCTAATTTTCGTTGCCGAAATCGACTTTGGTCAAGACTTTGCCAAAAATTTACAA
GAAAAATTTCAAAAAGAAAGAATGGCTTTTGTCGCTTCAACAACTAAAAGCAGAAAAACGATTGTCGAAGCTTTTCGTAA
GAAACAAGTTTCGATTTTAATTACGACTTCTATTCTTGAACGAGGCGTAACTTTTTCTTCGATTGATGTTTTTGTCATTA
ATAGCGAACACCCTAATTTCACAAAATCTGCCCTCATTCAAATGGTCGGACGAGTGGGACGAGACTCTAAACGTCCAACA
GGATTAGTTAGTTTTTTTCACTCTGGAAAATCTTTAGCCATGTGTCAAGCTCAGAAAGAAATTAAAAAAATGAACCAACT
CGGAGGTTTTTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(290-394)

Type III restriction enzyme, res subunit (ResIII)

(105-249)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A2RL94

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Lactococcus lactis subsp. cremoris KW2

97.674

100

0.977
  comFA/cflA Streptococcus mitis SK321

49.428

100

0.502
  comFA/cflA Streptococcus pneumoniae Rx1

49.428

100

0.502
  comFA/cflA Streptococcus pneumoniae D39

49.428

100

0.502
  comFA/cflA Streptococcus pneumoniae R6

49.428

100

0.502
  comFA/cflA Streptococcus pneumoniae TIGR4

49.428

100

0.502
  comFA/cflA Streptococcus mitis NCTC 12261

48.97

100

0.498
  comFA Bacillus subtilis subsp. subtilis str. 168

37.004

100

0.391

Multiple sequence alignment