Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   D2917_RS27675 Genome accession   NZ_CP032519
Coordinates   3223294..3225063 (+) Length   589 a.a.
NCBI ID   WP_151072543.1    Uniprot ID   A0A5P3VST8
Organism   Cupriavidus oxalaticus strain T2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3218294..3230063
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D2917_RS27640 (D2917_27520) proB 3218467..3219585 (-) 1119 WP_151072540.1 glutamate 5-kinase -
  D2917_RS27645 (D2917_27525) obgE 3219635..3220732 (-) 1098 WP_151072541.1 GTPase ObgE -
  D2917_RS27650 (D2917_27530) rpmA 3220856..3221116 (-) 261 WP_010814747.1 50S ribosomal protein L27 -
  D2917_RS27655 (D2917_27535) rplU 3221156..3221467 (-) 312 WP_006576525.1 50S ribosomal protein L21 -
  D2917_RS27660 (D2917_27540) - 3221663..3221956 (-) 294 WP_063241560.1 hypothetical protein -
  D2917_RS27665 (D2917_27545) ispB 3221955..3222884 (+) 930 WP_151072542.1 octaprenyl diphosphate synthase -
  D2917_RS27675 (D2917_27555) pilF 3223294..3225063 (+) 1770 WP_151072543.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  D2917_RS27680 (D2917_27560) - 3225143..3226414 (+) 1272 WP_063241557.1 type II secretion system F family protein -
  D2917_RS27685 (D2917_27565) pilD 3226418..3227320 (+) 903 WP_151072544.1 prepilin peptidase Machinery gene
  D2917_RS27690 (D2917_27570) coaE 3227372..3228010 (+) 639 WP_063241555.1 dephospho-CoA kinase -
  D2917_RS27695 (D2917_27575) zapD 3228248..3229006 (+) 759 WP_063241554.1 cell division protein ZapD -
  D2917_RS27700 (D2917_27580) - 3229011..3229202 (+) 192 WP_029048086.1 DNA gyrase inhibitor YacG -
  D2917_RS27705 (D2917_27585) - 3229256..3229693 (-) 438 WP_063241553.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 589 a.a.        Molecular weight: 64805.59 Da        Isoelectric Point: 7.7696

>NTDB_id=316637 D2917_RS27675 WP_151072543.1 3223294..3225063(+) (pilF) [Cupriavidus oxalaticus strain T2]
MTLGLALAQSRRIAPALLAQLEQAAREKQSQLIDEIVGSGTMSAHDLALFAADKYQLPLLDLNQYNLTKVPPGLAGNREF
HAHRLLPLGRRENRLVLGLSDPSNQAGLDAIKEKYKLPVEAVVVEHDKLMKYVRSAGEALGTLKNIAPVQAERKMIEYDP
VAAAGAQRNRTAADAIDDAPVVRFLQKLLTEAFHRGASDLHFEPFETFYRVRFRVDGVLQEVARPPLDIRDKIATRIKVL
SRLDISEKRVPQDGRMKLLIALPKDTKDNKDGKDAKDPKDKDARETVEKAVDFRVSTLPTLFGEKIVMRILESSSDKLDI
DQLGYEPQQKALLLDVIKRPYGMVLVTGPTGSGKTVSLYTFLNLLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFA
AALRSFLRQDPDVIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRLMNMGVAPFNIASSVLLITAQRLARRL
CTCKRPGELPRETLLDAGFREPDLDGSWQPYHPVGCERCNGSGYKGRCGIYQVMPITEAMQQIILSHGTALQIAEQARKD
GVLSLREAGLLKVRAGVTSLEEVLATTNT

Nucleotide


Download         Length: 1770 bp        

>NTDB_id=316637 D2917_RS27675 WP_151072543.1 3223294..3225063(+) (pilF) [Cupriavidus oxalaticus strain T2]
ATGACACTCGGTCTTGCCCTGGCCCAGAGCCGGCGTATCGCGCCCGCCCTGCTTGCTCAGCTGGAGCAGGCCGCCCGTGA
AAAGCAGTCGCAGCTGATCGATGAGATCGTCGGCAGCGGCACCATGAGCGCGCACGACCTGGCGCTGTTCGCGGCGGACA
AGTACCAGCTGCCGTTGCTGGACCTCAACCAGTACAACCTGACCAAGGTGCCGCCGGGGCTGGCGGGCAACCGCGAATTC
CATGCGCACCGCCTGCTGCCGCTGGGCCGGCGCGAAAACCGGTTGGTGCTGGGGCTGTCCGATCCGTCCAACCAGGCCGG
GCTGGACGCGATCAAGGAAAAGTACAAGCTGCCGGTCGAAGCGGTGGTGGTCGAGCACGACAAGCTGATGAAGTACGTGC
GCTCCGCCGGCGAGGCGCTGGGCACGCTCAAGAACATCGCGCCGGTGCAGGCCGAGCGCAAGATGATCGAATACGACCCG
GTGGCGGCCGCCGGCGCGCAGCGCAACCGCACTGCCGCCGATGCCATCGACGACGCCCCCGTGGTGCGCTTCCTGCAGAA
GCTGCTGACCGAAGCCTTCCACCGCGGCGCATCCGACCTGCATTTCGAGCCGTTCGAGACCTTCTACCGGGTCCGCTTCC
GCGTGGACGGCGTGCTGCAGGAGGTGGCAAGGCCGCCGCTGGATATCCGCGACAAGATCGCCACCCGCATCAAGGTGCTG
TCCCGGCTGGATATTTCTGAAAAGCGCGTGCCGCAGGACGGCCGCATGAAGCTGCTGATCGCGCTGCCCAAGGATACAAA
GGACAACAAGGACGGCAAGGACGCGAAGGATCCCAAGGACAAGGACGCCAGGGAAACCGTCGAGAAGGCCGTCGATTTCC
GCGTCTCGACGCTGCCGACGCTGTTCGGCGAGAAGATCGTGATGCGGATCCTGGAATCGTCGTCCGACAAGCTCGATATC
GACCAGCTCGGCTACGAGCCGCAGCAGAAGGCGCTGCTGCTGGACGTGATCAAGCGCCCGTACGGCATGGTGCTGGTGAC
CGGCCCCACCGGCAGCGGCAAGACGGTGTCGCTGTACACCTTCCTGAACCTGCTGAACCAGGGCGACATCAATATCTCGA
CCGCCGAGGACCCGGCCGAAATCCAGCTGCCCGGCATCAACCAGGTCAATGTCAACGACAAGGCCGGCCTGACCTTTGCC
GCGGCGCTGCGCTCGTTCCTGCGGCAGGATCCGGACGTCATCATGGTCGGCGAAATCCGCGACCTGGAGACCGCCGATAT
CTCGATCAAGGCCGCGCAGACCGGCCACCTGGTGCTGTCGACGCTGCACACCAACGACGCGCCCACCACGCTGACGCGGC
TGATGAACATGGGCGTGGCGCCGTTCAACATCGCCTCCAGCGTGCTGCTGATCACCGCGCAGCGGCTGGCGCGGCGGCTG
TGCACCTGCAAGCGCCCCGGCGAGCTGCCGCGCGAGACGCTGCTGGATGCGGGCTTCCGCGAACCTGATCTGGACGGCAG
CTGGCAGCCCTACCACCCGGTCGGGTGCGAGCGCTGCAACGGCAGCGGCTACAAGGGCCGCTGCGGCATCTACCAGGTCA
TGCCGATCACCGAGGCGATGCAGCAGATCATCCTGTCGCACGGCACCGCGCTGCAGATCGCCGAGCAGGCGCGCAAGGAC
GGCGTGCTATCGTTGCGCGAGGCTGGGCTGCTCAAGGTCAGAGCGGGCGTCACGTCACTCGAAGAGGTGCTGGCGACCAC
GAATACGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(285-479)

Type II/IV secretion system protein (T2SSE)

(187-260)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5P3VST8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

50.586

100

0.513
  pilB Acinetobacter baumannii D1279779

50.085

100

0.501
  pilB Acinetobacter baylyi ADP1

50.265

95.925

0.482
  pilB Legionella pneumophila strain ERS1305867

49.027

95.925

0.47
  pilB Vibrio cholerae strain A1552

43.919

100

0.441
  pilB Vibrio campbellii strain DS40M4

45.324

94.397

0.428
  pilB Vibrio parahaemolyticus RIMD 2210633

45.307

94.058

0.426
  pilF Thermus thermophilus HB27

38.345

100

0.385
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.852

96.435

0.365

Multiple sequence alignment