Detailed information NTG0316638

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: D2917_RS27685
• Genome accession: NZ_CP032519
• Coordinates: 3226418..3227320 (+)
• Length: 300 a.a.
• NCBI ID: WP_151072544.1
• Uniprot ID: A0A5P3VRF0
• Organism: Cupriavidus oxalaticus strain T2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3221418..3232320

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D2917_RS27660 (D2917_27540)	-	3221663..3221956 (-)	294	WP_063241560.1	hypothetical protein	-
D2917_RS27665 (D2917_27545)	ispB	3221955..3222884 (+)	930	WP_151072542.1	octaprenyl diphosphate synthase	-
D2917_RS27675 (D2917_27555)	pilF	3223294..3225063 (+)	1770	WP_151072543.1	ATPase, T2SS/T4P/T4SS family	Machinery gene
D2917_RS27680 (D2917_27560)	-	3225143..3226414 (+)	1272	WP_063241557.1	type II secretion system F family protein	-
D2917_RS27685 (D2917_27565)	pilD	3226418..3227320 (+)	903	WP_151072544.1	prepilin peptidase	Machinery gene
D2917_RS27690 (D2917_27570)	coaE	3227372..3228010 (+)	639	WP_063241555.1	dephospho-CoA kinase	-
D2917_RS27695 (D2917_27575)	zapD	3228248..3229006 (+)	759	WP_063241554.1	cell division protein ZapD	-
D2917_RS27700 (D2917_27580)	-	3229011..3229202 (+)	192	WP_029048086.1	DNA gyrase inhibitor YacG	-
D2917_RS27705 (D2917_27585)	-	3229256..3229693 (-)	438	WP_063241553.1	NUDIX domain-containing protein	-
D2917_RS27710 (D2917_27590)	-	3229690..3230607 (-)	918	WP_151072545.1	ATP-binding protein	-
D2917_RS27715 (D2917_27595)	argJ	3230668..3231906 (-)	1239	WP_151072546.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-

Sequence

Protein

• Length: 300 a.a.
• Molecular weight: 32016.23 Da
• Isoelectric Point: 8.3382

>NTDB_id=316638 D2917_RS27685 WP_151072544.1 3226418..3227320(+) (pilD) [Cupriavidus oxalaticus strain T2]
MALAYSVSPFPAGSDQLLQALAVLPPAFMVAVTAVLGLVVGSFLNVVIHRLPRMMEREEANYIAELRGDPLPYPARYNLM
VPRSACPHCGHAIAPWENVPVLSWLALRGRCSACKARISARYPLVELACGVLSALVAWRFGPGVQALAALVLVWALLALT
LIDADTQLLPDQITLPLLWLGLVLNLGGLFVPLADAVIGAAAGYLVLWTAYWLFRLVRGKEGMGFGDFKLMAALGAWFGW
QALPALVLLSSVAGVAFGLAGIALRRQDRDTPFPFGPFIALAGLVVLVFGSGVLPLTLLP

Nucleotide

• Length: 903 bp

>NTDB_id=316638 D2917_RS27685 WP_151072544.1 3226418..3227320(+) (pilD) [Cupriavidus oxalaticus strain T2]
ATGGCGCTGGCGTATTCCGTTTCGCCGTTTCCCGCAGGCAGCGATCAATTGCTGCAGGCGCTGGCGGTGCTGCCGCCTGC
CTTCATGGTGGCGGTGACGGCCGTGCTCGGCCTGGTGGTGGGCAGTTTCCTCAACGTGGTGATCCACCGGCTGCCGCGCA
TGATGGAGCGCGAGGAAGCCAACTATATCGCCGAGCTGCGCGGCGATCCGCTGCCCTACCCCGCCCGCTACAACCTGATG
GTGCCGCGCTCGGCCTGTCCGCACTGCGGCCACGCGATCGCGCCCTGGGAAAACGTGCCGGTGCTGAGCTGGCTGGCGCT
GCGCGGGCGCTGCTCGGCCTGCAAGGCCCGGATCAGCGCACGCTATCCGCTGGTGGAGCTGGCCTGCGGCGTGCTGAGCG
CGCTGGTGGCGTGGCGCTTCGGCCCTGGCGTGCAGGCCTTGGCGGCACTGGTGCTGGTCTGGGCGCTGCTGGCGCTGACC
CTGATCGATGCCGATACCCAGTTGCTGCCGGACCAGATCACGCTGCCGCTGCTGTGGCTGGGACTGGTGCTGAACCTGGG
CGGGTTGTTCGTGCCGCTGGCCGACGCCGTGATCGGCGCCGCCGCCGGCTACCTGGTGCTGTGGACGGCGTACTGGCTGT
TCCGGCTGGTGCGTGGCAAGGAGGGCATGGGTTTCGGCGATTTCAAGCTGATGGCGGCACTCGGGGCGTGGTTTGGCTGG
CAGGCGCTGCCGGCGCTGGTGCTGCTGTCATCGGTGGCCGGTGTGGCGTTCGGGCTGGCCGGCATCGCACTGCGCCGGCA
GGATCGCGACACGCCCTTCCCTTTCGGCCCCTTTATCGCGCTGGCGGGCCTGGTGGTGCTGGTGTTCGGCTCCGGCGTGC
TGCCGCTGACCTTGCTGCCTTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (150-259)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (35-140)

3D structure

• AlphaFold DB: A0A5P3VRF0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	54.779	90.667	0.497
pilD	Vibrio campbellii strain DS40M4	51.825	91.333	0.473
pilD	Neisseria gonorrhoeae MS11	48.921	92.667	0.453
pilD	Acinetobacter baumannii D1279779	46.715	91.333	0.427
pilD	Acinetobacter nosocomialis M2	46.35	91.333	0.423