Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CDG62_RS15800 Genome accession   NZ_CP032286
Coordinates   2703048..2704169 (+) Length   373 a.a.
NCBI ID   WP_087528299.1    Uniprot ID   A0A385N9L6
Organism   Acinetobacter sp. WCHA55     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2698048..2709169
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CDG62_RS15785 (CDG62_15790) - 2700247..2700981 (-) 735 WP_087528297.1 hypothetical protein -
  CDG62_RS15790 (CDG62_15795) - 2701172..2701861 (-) 690 WP_087528298.1 YggS family pyridoxal phosphate-dependent enzyme -
  CDG62_RS15795 (CDG62_15800) pilT 2701984..2703021 (+) 1038 WP_004694862.1 type IV pilus twitching motility protein PilT Machinery gene
  CDG62_RS15800 (CDG62_15805) pilU 2703048..2704169 (+) 1122 WP_087528299.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CDG62_RS15805 (CDG62_15810) fur 2704229..2704666 (-) 438 WP_004694866.1 ferric iron uptake transcriptional regulator -
  CDG62_RS15810 (CDG62_15815) - 2704779..2705177 (+) 399 WP_087528300.1 outer membrane protein assembly factor BamE -
  CDG62_RS15815 (CDG62_15820) - 2705224..2705547 (-) 324 WP_087528301.1 RnfH family protein -
  CDG62_RS15820 (CDG62_15825) - 2705547..2706629 (-) 1083 WP_087528302.1 hypothetical protein -
  CDG62_RS15825 (CDG62_15830) - 2706772..2707227 (-) 456 WP_087528303.1 bacteriohemerythrin -
  CDG62_RS15830 (CDG62_15835) - 2707502..2708350 (-) 849 WP_087528304.1 class II glutamine amidotransferase -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41916.21 Da        Isoelectric Point: 6.7280

>NTDB_id=314751 CDG62_RS15800 WP_087528299.1 2703048..2704169(+) (pilU) [Acinetobacter sp. WCHA55]
MDFNDLLNYMIQQKASDLFITADVEPSMKINGQIVPVAKGKLTGDVAGQLLHSIMSEKQRKEFADTRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETQIPTMDELKLPPVLKELAMTKRGIIIFVGATGTGKSTSLASIIGYRNQNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEVALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIER
IIHFFETDRHSQLFMDLSLNLKAMVAQQLIPTPDGNSRRAAIEILINSPLISDLIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAKQITYKDALKHADSPNDLRLQIKLSEEGGDRLLHASANITFDGQA

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=314751 CDG62_RS15800 WP_087528299.1 2703048..2704169(+) (pilU) [Acinetobacter sp. WCHA55]
ATGGATTTTAACGACTTACTCAATTATATGATTCAACAAAAAGCGTCGGATTTATTTATCACCGCTGATGTTGAACCCTC
CATGAAAATTAACGGTCAAATTGTGCCTGTGGCTAAGGGCAAGCTGACGGGTGATGTTGCGGGGCAACTGCTCCACTCGA
TCATGAGTGAGAAACAGCGCAAAGAATTTGCAGATACACGTGAGTGTAACTTCGCGATTATGAATCGGGATAAAACTGCA
CGCTTTCGTGTCAGTGCCTTTCAACAGCGTGACATGCCAGGTATGGTTTTGCGTAGGATTGAGACGCAAATTCCAACCAT
GGACGAGTTAAAGCTCCCGCCTGTGCTCAAAGAGTTAGCCATGACCAAACGTGGCATCATCATCTTTGTGGGTGCAACAG
GTACAGGTAAATCGACCTCTTTGGCTTCGATCATTGGTTACCGTAACCAAAACTCGAAAGGTCATATCATTACCATTGAA
GACCCGATTGAATTTATTCATGAGCATGCGGGCTGTATTATTACCCAGCGTGAAGTAGGCATTGACACTGACTCCTTTGA
AGTGGCTTTGAAAAACACCTTGCGTCAAGCACCTGATGTGATCTTGATCGGTGAGATTCGTTCCCGAGAAACCATGGATT
ATGCGATTGCCTTTGCTGAAACAGGTCACTTGGTATTTGCAACGCTGCATGCCAACAATGCTAACCAAGCCATCGAGCGT
ATTATTCACTTTTTTGAAACAGACCGTCATAGCCAATTGTTTATGGACTTGTCTTTAAACCTTAAAGCGATGGTTGCTCA
GCAGTTGATTCCAACACCAGACGGTAATTCTCGTCGTGCTGCAATTGAGATTTTGATTAACTCACCGTTAATTTCCGATT
TGATCCGTAAAGGTGAAATCCACGAAATTAAAGACCTGATGAAGCGTTCTCGTGAACTCGGTATGCAAACTTTTGACCAA
GCCTTATTCGATTTGTACAAAGCTAAGCAGATTACCTATAAAGATGCGCTGAAACACGCCGACTCTCCAAATGACTTACG
ATTACAAATCAAGTTGTCTGAAGAAGGCGGTGATCGTCTATTGCATGCTAGTGCAAACATCACATTTGACGGACAAGCTT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A385N9L6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

76.455

100

0.775
  pilU Pseudomonas stutzeri DSM 10701

68.194

99.464

0.678
  pilU Vibrio cholerae strain A1552

55.946

99.196

0.555
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.197

92.761

0.391
  pilT Legionella pneumophila strain Lp02

40.828

90.617

0.37
  pilT Legionella pneumophila strain ERS1305867

40.828

90.617

0.37

Multiple sequence alignment