Detailed information NTG0313936

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: D3C60_RS12360
• Genome accession: NZ_CP032144
• Coordinates: 2530880..2531494 (-)
• Length: 204 a.a.
• NCBI ID: WP_032866693.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain BIM B-439D
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2525880..2536494

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D3C60_RS12335 (D3C60_12335)	rpsT	2526183..2526449 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
D3C60_RS12340 (D3C60_12340)	holA	2526466..2527507 (-)	1042	Protein_2369	DNA polymerase III subunit delta	-
D3C60_RS19975	-	2527565..2527698 (-)	134	Protein_2370	hypothetical protein	-
D3C60_RS12345 (D3C60_12345)	-	2527739..2527873 (+)	135	WP_003152870.1	YqzM family protein	-
D3C60_RS12350 (D3C60_12350)	comEC	2527892..2530243 (-)	2352	WP_032866696.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
D3C60_RS12355 (D3C60_12355)	-	2530244..2530813 (-)	570	WP_003152868.1	ComE operon protein 2	-
D3C60_RS12360 (D3C60_12360)	comEA	2530880..2531494 (-)	615	WP_032866693.1	helix-hairpin-helix domain-containing protein	Machinery gene
D3C60_RS12365 (D3C60_12365)	comER	2531553..2532374 (+)	822	WP_032866690.1	late competence protein ComER	-
D3C60_RS12370 (D3C60_12370)	-	2532443..2533180 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
D3C60_RS12375 (D3C60_12375)	rsfS	2533177..2533533 (-)	357	WP_007408260.1	ribosome silencing factor	-
D3C60_RS12380 (D3C60_12380)	yqeK	2533551..2534111 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
D3C60_RS12385 (D3C60_12385)	-	2534101..2534670 (-)	570	WP_032866687.1	nicotinate-nucleotide adenylyltransferase	-
D3C60_RS12390 (D3C60_12390)	yhbY	2534681..2534971 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
D3C60_RS12395 (D3C60_12395)	aroE	2534965..2535807 (-)	843	WP_007408256.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22151.28 Da
• Isoelectric Point: 6.4858

>NTDB_id=313936 D3C60_RS12360 WP_032866693.1 2530880..2531494(-) (comEA) [Bacillus velezensis strain BIM B-439D]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSDKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=313936 D3C60_RS12360 WP_032866693.1 2530880..2531494(-) (comEA) [Bacillus velezensis strain BIM B-439D]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGACAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTTAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCATACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	32.759	100	0.373
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363