Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AhaeAN4_RS15120 Genome accession   NZ_CP031979
Coordinates   3086128..3087843 (+) Length   571 a.a.
NCBI ID   WP_005083851.1    Uniprot ID   A0A380UBG2
Organism   Acinetobacter haemolyticus strain AN4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3081128..3092843
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AhaeAN4_RS15085 (AhaeAN4_15170) nusA 3081935..3083419 (-) 1485 WP_005088007.1 transcription termination factor NusA -
  AhaeAN4_RS15090 (AhaeAN4_15175) rimP 3083457..3083981 (-) 525 WP_005083847.1 ribosome maturation factor RimP -
  AhaeAN4_RS15110 (AhaeAN4_15195) secG 3084618..3084947 (-) 330 WP_004637686.1 preprotein translocase subunit SecG -
  AhaeAN4_RS15115 (AhaeAN4_15200) tpiA 3084960..3085751 (-) 792 WP_005088004.1 triose-phosphate isomerase -
  AhaeAN4_RS15120 (AhaeAN4_15205) pilB 3086128..3087843 (+) 1716 WP_005083851.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AhaeAN4_RS15125 (AhaeAN4_15210) pilC 3087868..3089094 (+) 1227 WP_005083853.1 type II secretion system F family protein Machinery gene
  AhaeAN4_RS15130 (AhaeAN4_15215) pilD 3089094..3089954 (+) 861 WP_005083855.1 prepilin peptidase Machinery gene
  AhaeAN4_RS15135 (AhaeAN4_15220) coaE 3089956..3090561 (+) 606 WP_005088002.1 dephospho-CoA kinase -
  AhaeAN4_RS15140 (AhaeAN4_15225) - 3090558..3091469 (-) 912 WP_017394516.1 DMT family transporter -
  AhaeAN4_RS15145 (AhaeAN4_15235) rlmB 3091509..3092255 (-) 747 WP_004637678.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  AhaeAN4_RS15150 (AhaeAN4_15240) - 3092364..3092690 (-) 327 WP_004637677.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63323.54 Da        Isoelectric Point: 5.1480

>NTDB_id=313100 AhaeAN4_RS15120 WP_005083851.1 3086128..3087843(+) (pilB) [Acinetobacter haemolyticus strain AN4]
MSGLHTSPKFSGFIRRLVEDGHMSASTMQTAIDTAKKTQQDIVGYLIEHHRISQLTIAESISAEFGEPIFDLAVFDTALL
PRELADSKLIQKHRVAPLIQRGQILYVATSNPTNIDALDAMRFNTKLNIEPIIVEHSKLEKLIEQQFGDSGSFDFGDSED
FDLDIDVSQDSQKDESDETPQGDESPIVKYINKLLIDAIRMGASDLHFEPYEKTYRVRYRVDGVLRQIATPPLQMANRLA
SRLKVMSQMDISEKRMPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIEALGYEDEQKALFMEALEK
PQGMLLITGPTGSGKTVSLYTGLNILNTEDTNISTAEDPVEINLEGINQVNVNTKTGLTFSAALKAFLRQDPDIIMVGEI
RDLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSHCKAPIEIPKQSLLELG
FTEQDLADPELQIFQPVGCSECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAAASEKAGFNNLRRSGLKKVMQGIT
SLQEINRVTSE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=313100 AhaeAN4_RS15120 WP_005083851.1 3086128..3087843(+) (pilB) [Acinetobacter haemolyticus strain AN4]
ATGTCAGGATTACATACGTCTCCAAAATTTTCGGGGTTTATTCGACGGTTAGTCGAAGATGGTCATATGTCTGCATCGAC
AATGCAAACTGCAATCGATACAGCAAAAAAAACACAACAAGATATTGTTGGATATTTAATTGAACATCATCGTATCTCTC
AACTCACCATTGCAGAGTCAATCTCGGCAGAGTTTGGCGAACCTATTTTTGATTTAGCTGTTTTTGATACTGCTTTATTA
CCTCGTGAGCTTGCTGACAGCAAATTAATTCAAAAGCATCGGGTTGCCCCTCTCATTCAACGCGGACAAATTCTTTATGT
CGCGACAAGCAATCCAACCAATATTGACGCGCTTGATGCGATGCGATTTAACACCAAGCTCAATATTGAACCGATTATCG
TCGAACACAGTAAACTTGAAAAACTGATTGAACAGCAGTTTGGTGACTCTGGAAGTTTTGATTTTGGTGACAGTGAAGAC
TTTGACTTAGATATTGATGTCAGCCAAGATTCGCAAAAAGATGAAAGTGATGAAACGCCTCAAGGGGATGAATCACCAAT
TGTAAAATACATCAACAAGTTACTGATTGATGCCATTCGCATGGGTGCGTCTGATCTACATTTTGAACCCTATGAAAAAA
CCTATCGTGTTCGTTATCGCGTAGATGGTGTCTTAAGACAAATTGCGACGCCACCGTTGCAAATGGCAAATCGCTTAGCT
TCCCGTTTAAAAGTCATGTCTCAAATGGACATTTCTGAAAAACGAATGCCGCAAGATGGTCGTATTAAGCTCAAACTTTC
GAAAACCAAAGCGATTGACTTTCGTGTCAACTCACTGCCTACCCTATTTGGTGAAAAGCTAGTACTCCGTATTCTGGATC
CTTCCAGCGCCATGCTCGGAATTGAGGCACTCGGTTACGAGGATGAACAAAAAGCTCTATTTATGGAGGCGCTTGAAAAA
CCTCAAGGCATGCTGTTAATTACTGGCCCAACAGGTTCTGGTAAAACTGTGTCGTTATATACAGGTCTCAACATTCTAAA
TACTGAAGACACCAATATTTCTACTGCTGAAGACCCAGTGGAGATTAACTTAGAAGGAATCAATCAGGTTAACGTCAATA
CTAAGACAGGCCTTACCTTCTCTGCTGCGCTGAAAGCATTCTTACGTCAAGATCCAGACATTATCATGGTCGGTGAGATT
CGTGACCTAGAAACAGCAGAAATTGCAATCAAGGCTGCTCAAACGGGTCATATGGTGATGTCCACGCTACATACCAATAG
TGCACCTGAAACACTGACGCGTTTACGCAATATGGGTGTTCCTTCTTTCAATATCGCAACTTCGGTCAATCTTGTGATTG
CACAACGTTTAGCCCGTCGTTTATGCTCACACTGTAAAGCACCCATAGAGATCCCCAAACAAAGCTTATTAGAACTCGGC
TTTACTGAACAGGATTTAGCTGACCCTGAATTGCAAATCTTTCAACCAGTCGGCTGCTCAGAATGTCGTGAAGGCTATAA
AGGTCGTGTCGGTATCTATGAGGTCATGAAGGTGACGCCTGAGATCTCTAAAATTATTATGGAGGATGGCAATGCTTTAC
AAATCGCAGCGGCATCAGAAAAAGCAGGATTTAATAATTTAAGACGCTCAGGTTTAAAGAAGGTCATGCAAGGCATTACC
TCATTGCAAGAAATTAACCGCGTCACAAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(193-460)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A380UBG2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

82.137

100

0.821
  pilB Acinetobacter baylyi ADP1

77.583

100

0.776
  pilB Legionella pneumophila strain ERS1305867

53.659

93.345

0.501
  pilF Neisseria gonorrhoeae MS11

48.522

100

0.489
  pilB Vibrio parahaemolyticus RIMD 2210633

49.016

97.898

0.48
  pilB Vibrio cholerae strain A1552

48.644

96.848

0.471
  pilB Vibrio campbellii strain DS40M4

51.06

90.893

0.464
  pilF Thermus thermophilus HB27

37.478

98.599

0.37
  pilB/pilB1 Synechocystis sp. PCC 6803

33.659

100

0.363
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.795

92.995

0.361

Multiple sequence alignment