Detailed information NTG0313021

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: AhaeAN59_RS14840
• Genome accession: NZ_CP031972
• Coordinates: 3121329..3123044 (+)
• Length: 571 a.a.
• NCBI ID: WP_005083851.1
• Uniprot ID: A0A380UBG2
• Organism: Acinetobacter haemolyticus strain AN59
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IS/Tn	3123782..3124483	3121329..3123044	flank	738

Gene organization within MGE regions

Location: 3121329..3124483

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AhaeAN59_RS14840 (AhaeAN59_14995)	pilB	3121329..3123044 (+)	1716	WP_005083851.1	type IV-A pilus assembly ATPase PilB	Machinery gene
AhaeAN59_RS14845 (AhaeAN59_15000)	-	3123069..3123761 (+)	693	Protein_2913	type II secretion system F family protein	-
AhaeAN59_RS14850 (AhaeAN59_15005)	-	3123782..3124483 (-)	702	WP_001223318.1	IS1-like element ISPa14 family transposase	-

Sequence

Protein

• Length: 571 a.a.
• Molecular weight: 63323.54 Da
• Isoelectric Point: 5.1480

>NTDB_id=313021 AhaeAN59_RS14840 WP_005083851.1 3121329..3123044(+) (pilB) [Acinetobacter haemolyticus strain AN59]
MSGLHTSPKFSGFIRRLVEDGHMSASTMQTAIDTAKKTQQDIVGYLIEHHRISQLTIAESISAEFGEPIFDLAVFDTALL
PRELADSKLIQKHRVAPLIQRGQILYVATSNPTNIDALDAMRFNTKLNIEPIIVEHSKLEKLIEQQFGDSGSFDFGDSED
FDLDIDVSQDSQKDESDETPQGDESPIVKYINKLLIDAIRMGASDLHFEPYEKTYRVRYRVDGVLRQIATPPLQMANRLA
SRLKVMSQMDISEKRMPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIEALGYEDEQKALFMEALEK
PQGMLLITGPTGSGKTVSLYTGLNILNTEDTNISTAEDPVEINLEGINQVNVNTKTGLTFSAALKAFLRQDPDIIMVGEI
RDLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSHCKAPIEIPKQSLLELG
FTEQDLADPELQIFQPVGCSECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAAASEKAGFNNLRRSGLKKVMQGIT
SLQEINRVTSE

Nucleotide

• Length: 1716 bp

>NTDB_id=313021 AhaeAN59_RS14840 WP_005083851.1 3121329..3123044(+) (pilB) [Acinetobacter haemolyticus strain AN59]
ATGTCAGGATTACATACGTCTCCAAAATTTTCGGGGTTTATTCGACGGTTAGTCGAAGATGGTCATATGTCTGCATCGAC
AATGCAAACTGCAATCGATACAGCAAAAAAAACACAACAAGATATTGTTGGATATTTAATTGAACATCATCGTATCTCTC
AACTCACCATTGCAGAGTCAATCTCGGCAGAGTTTGGCGAACCTATTTTTGATTTAGCTGTTTTTGATACTGCTTTATTA
CCTCGTGAGCTTGCTGACAGCAAATTAATTCAAAAGCATCGGGTTGCCCCTCTCATTCAACGCGGACAAATTCTTTATGT
CGCGACAAGCAATCCAACCAATATTGACGCGCTTGATGCGATGCGATTTAACACCAAGCTCAATATTGAACCGATTATCG
TCGAACACAGTAAACTTGAAAAACTGATTGAACAGCAGTTTGGTGACTCTGGAAGTTTTGATTTTGGTGACAGTGAAGAC
TTTGACTTAGATATTGATGTCAGCCAAGATTCGCAAAAAGATGAAAGTGATGAAACGCCTCAAGGGGATGAATCACCAAT
TGTAAAATACATCAACAAGTTACTGATTGATGCCATTCGCATGGGTGCGTCTGATCTACATTTTGAACCCTATGAAAAAA
CCTATCGTGTTCGTTATCGCGTAGATGGTGTCTTAAGACAAATTGCGACGCCACCGTTGCAAATGGCAAATCGCTTAGCT
TCCCGTTTAAAAGTCATGTCTCAAATGGACATTTCTGAAAAACGAATGCCGCAAGATGGTCGTATTAAGCTCAAACTTTC
GAAAACCAAAGCGATTGACTTTCGTGTCAACTCACTGCCTACCCTATTTGGTGAAAAGCTAGTACTCCGTATTCTGGATC
CTTCCAGCGCCATGCTCGGAATTGAGGCACTCGGTTACGAGGATGAACAAAAAGCTCTATTTATGGAGGCGCTTGAAAAA
CCTCAAGGCATGCTGTTAATTACTGGCCCAACAGGTTCTGGTAAAACTGTGTCGTTATATACAGGTCTCAACATTCTAAA
TACTGAAGACACCAATATTTCTACTGCTGAAGACCCAGTGGAGATTAACTTAGAAGGAATCAATCAGGTTAACGTCAATA
CTAAGACAGGCCTTACCTTCTCTGCTGCGCTGAAAGCATTCTTACGTCAAGATCCAGACATTATCATGGTCGGTGAGATT
CGTGACCTAGAAACAGCAGAAATTGCAATCAAGGCTGCTCAAACGGGTCATATGGTGATGTCCACGCTACATACCAATAG
TGCACCTGAAACACTGACGCGTTTACGCAATATGGGTGTTCCTTCTTTCAATATCGCAACTTCGGTCAATCTTGTGATTG
CACAACGTTTAGCCCGTCGTTTATGCTCACACTGTAAAGCACCCATAGAGATCCCCAAACAAAGCTTATTAGAACTCGGC
TTTACTGAACAGGATTTAGCTGACCCTGAATTGCAAATCTTTCAACCAGTCGGCTGCTCAGAATGTCGTGAAGGCTATAA
AGGTCGTGTCGGTATCTATGAGGTCATGAAGGTGACGCCTGAGATCTCTAAAATTATTATGGAGGATGGCAATGCTTTAC
AAATCGCAGCGGCATCAGAAAAAGCAGGATTTAATAATTTAAGACGCTCAGGTTTAAAGAAGGTCATGCAAGGCATTACC
TCATTGCAAGAAATTAACCGCGTCACAAGTGAATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (193-460)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (46-149)

3D structure

• AlphaFold DB: A0A380UBG2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	82.137	100	0.821
pilB	Acinetobacter baylyi ADP1	77.583	100	0.776
pilB	Legionella pneumophila strain ERS1305867	53.659	93.345	0.501
pilF	Neisseria gonorrhoeae MS11	48.522	100	0.489
pilB	Vibrio parahaemolyticus RIMD 2210633	49.016	97.898	0.48
pilB	Vibrio cholerae strain A1552	48.644	96.848	0.471
pilB	Vibrio campbellii strain DS40M4	51.06	90.893	0.464
pilF	Thermus thermophilus HB27	37.478	98.599	0.37
pilB/pilB1	Synechocystis sp. PCC 6803	33.659	100	0.363
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.795	92.995	0.361