Detailed information NTG0312764

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: LL223_RS09655
• Genome accession: NZ_CP031926
• Coordinates: 1894816..1895463 (-)
• Length: 215 a.a.
• NCBI ID: WP_010906131.1
• Uniprot ID: A0A3N6KQH1
• Organism: Lactococcus lactis subsp. lactis strain 223
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1889816..1900463

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LL223_RS09630 (LL223_1847)	atpF	1889886..1890392 (-)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-
LL223_RS09635 (LL223_1848)	atpB	1890407..1891120 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
LL223_RS09640 (LL223_1849)	-	1891165..1891380 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
LL223_RS09645 (LL223_1850)	-	1891564..1892340 (-)	777	WP_004255244.1	alpha/beta hydrolase family protein	-
LL223_RS09650 (LL223_1851)	comEC	1892625..1894835 (-)	2211	WP_010906130.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
LL223_RS09655 (LL223_1852)	comEA	1894816..1895463 (-)	648	WP_010906131.1	ComEA family DNA-binding protein	Machinery gene
LL223_RS09660 (LL223_1853)	-	1895523..1896884 (-)	1362	WP_021214597.1	ABC transporter permease	-
LL223_RS09665 (LL223_1854)	-	1896881..1897813 (-)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
LL223_RS09670 (LL223_1855)	-	1897918..1898316 (-)	399	WP_004255236.1	hypothetical protein	-
LL223_RS09675 (LL223_1856)	-	1898420..1898983 (-)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
LL223_RS09680 (LL223_1857)	-	1899156..1900334 (-)	1179	WP_021214596.1	SLC13 family permease	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23624.76 Da
• Isoelectric Point: 4.8659

>NTDB_id=312764 LL223_RS09655 WP_010906131.1 1894816..1895463(-) (comEA) [Lactococcus lactis subsp. lactis strain 223]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=312764 LL223_RS09655 WP_010906131.1 1894816..1895463(-) (comEA) [Lactococcus lactis subsp. lactis strain 223]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATTTAATTAAAGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (151-212)

3D structure

• AlphaFold DB: A0A3N6KQH1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	76.147	100	0.772
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	36.564	100	0.386