Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   D1112_RS04400 Genome accession   NZ_CP031854
Coordinates   987886..989280 (-) Length   464 a.a.
NCBI ID   WP_005601209.1    Uniprot ID   B0BPG4
Organism   Actinobacillus pleuropneumoniae strain ORG1224     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 982886..994280
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D1112_RS04370 (D1112_04390) lptE 983500..983994 (+) 495 WP_005597385.1 LPS assembly lipoprotein LptE -
  D1112_RS04375 (D1112_04395) holA 984103..985128 (+) 1026 WP_012263005.1 DNA polymerase III subunit delta -
  D1112_RS04380 (D1112_04400) yacG 985205..985393 (-) 189 WP_005597388.1 DNA gyrase inhibitor YacG -
  D1112_RS04385 (D1112_04405) coaE 985375..986013 (-) 639 WP_005601202.1 dephospho-CoA kinase -
  D1112_RS04390 (D1112_04410) - 986035..986673 (-) 639 WP_005604550.1 prepilin peptidase -
  D1112_RS04395 (D1112_04415) pilC 986700..987902 (-) 1203 WP_012263006.1 type II secretion system F family protein Machinery gene
  D1112_RS04400 (D1112_04420) pilB 987886..989280 (-) 1395 WP_005601209.1 GspE/PulE family protein Machinery gene
  D1112_RS04405 (D1112_04425) pilA 989298..989744 (-) 447 WP_005597397.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  D1112_RS04410 (D1112_04430) radA 989926..991305 (+) 1380 WP_237601081.1 DNA repair protein RadA -
  D1112_RS04415 (D1112_04435) - 991349..991582 (-) 234 WP_005597399.1 YgjV family protein -
  D1112_RS04420 (D1112_04440) folA 991709..992197 (-) 489 WP_012263007.1 type 3 dihydrofolate reductase -
  D1112_RS04425 (D1112_04445) proB 992284..993375 (+) 1092 WP_237586572.1 glutamate 5-kinase -
  D1112_RS04430 (D1112_04450) - 993388..994164 (+) 777 WP_237586573.1 TSUP family transporter -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 53022.90 Da        Isoelectric Point: 6.5042

>NTDB_id=311892 D1112_RS04400 WP_005601209.1 987886..989280(-) (pilB) [Actinobacillus pleuropneumoniae strain ORG1224]
MQYSVCDVNNRQVFRISEEQWQKNYDERHILLRYLTVPLREDQQTLWLAIDDMNNLSACEIFAFLTHKRIEPVLVSSEEL
KYLLGQLMPEQQSVYDETELPYQPLAQAQHSDVNDPIIQVLDGLFKYGLKHNASDIHLEPQKDKMLIRLRIDGVLHPYKS
FSLQLANRLISRIKLLAKLDISESRLPQDGQFGFKTILSETLDFRVSSLPTYWGEKVVLRLQKNKPTHFDFTTLGFLAEQ
KEKLLRALRQPQGLILVTGPTGSGKSITLYSALDYLNDSKRHILTAEDPIEIELEGIIQTQINPAIDLGFSRLLRTFLRQ
DPDVIMLGEIRDQESAEIALSAAQTGHLVLSTLHTNDAPSAIERLLQLGIKEYEINNSLLLVVAQRLVRKLCTECGGKGC
KYCYHGYRGRIGVYQLFSKNGKIFEKSTACLDFENLYQSAQQKFKTGITSLEEIERVLGHVESV

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=311892 D1112_RS04400 WP_005601209.1 987886..989280(-) (pilB) [Actinobacillus pleuropneumoniae strain ORG1224]
ATGCAGTATTCCGTATGTGATGTAAACAATCGGCAAGTATTTCGGATCTCCGAGGAACAGTGGCAAAAAAATTATGATGA
ACGTCATATCTTATTACGTTATCTCACCGTACCGCTTCGGGAAGACCAACAAACATTATGGTTGGCAATCGATGATATGA
ATAATCTCAGTGCTTGCGAGATTTTTGCTTTTCTTACGCATAAGCGAATTGAGCCGGTATTGGTCTCTTCCGAAGAATTG
AAATACCTGCTCGGGCAATTAATGCCGGAACAACAATCGGTATATGACGAAACGGAATTACCTTATCAACCTTTAGCACA
AGCGCAGCATTCGGATGTTAATGATCCGATTATTCAAGTGTTGGACGGCCTGTTTAAATACGGTTTAAAACATAATGCTT
CGGATATTCATTTGGAACCGCAAAAAGACAAAATGTTGATTCGATTACGTATCGACGGCGTGTTACATCCGTATAAATCA
TTTTCTCTCCAACTTGCCAATCGACTTATTTCTCGCATTAAATTATTAGCTAAACTGGATATTAGTGAATCCCGTCTTCC
GCAAGACGGGCAATTCGGTTTTAAGACAATCTTATCCGAAACATTGGATTTCCGTGTTTCGAGTTTACCGACTTATTGGG
GCGAAAAAGTCGTTTTACGGCTACAAAAAAATAAACCGACGCATTTTGATTTTACTACGTTGGGCTTCTTGGCGGAGCAG
AAGGAAAAGTTGTTGCGGGCATTGCGGCAGCCTCAAGGATTAATATTAGTTACGGGACCGACCGGCAGTGGTAAAAGTAT
TACCTTATATAGTGCGTTGGATTATTTAAATGACAGTAAAAGGCATATTTTGACCGCCGAAGATCCGATAGAAATCGAAC
TTGAAGGCATAATCCAAACGCAAATAAACCCGGCGATAGATTTAGGGTTCAGCCGGCTATTAAGAACGTTTTTACGACAA
GATCCGGATGTGATTATGTTAGGTGAAATTCGTGATCAAGAAAGTGCCGAAATTGCATTAAGTGCGGCACAAACCGGGCA
TTTAGTGCTTTCGACCTTACATACCAATGATGCGCCTTCGGCAATCGAACGCTTATTGCAGCTCGGTATCAAAGAATATG
AAATCAATAATTCGTTATTACTTGTCGTGGCGCAACGTTTAGTTCGTAAATTATGTACCGAATGCGGCGGAAAAGGCTGT
AAATATTGTTATCACGGCTACAGAGGCAGAATCGGCGTTTATCAACTGTTTTCAAAAAATGGAAAAATTTTTGAGAAATC
GACCGCTTGTTTGGATTTTGAAAACTTATATCAAAGTGCGCAACAGAAATTCAAGACGGGAATCACTTCATTAGAAGAAA
TCGAGAGAGTGTTAGGTCATGTTGAAAGTGTATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-390)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(28-92)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B0BPG4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

68.192

98.922

0.675
  pilB Haemophilus influenzae Rd KW20

58.72

97.629

0.573
  pilB Haemophilus influenzae 86-028NP

58.278

97.629

0.569
  pilB Vibrio campbellii strain DS40M4

40.125

100

0.416
  pilB Legionella pneumophila strain ERS1305867

39.264

100

0.414
  pilB Vibrio parahaemolyticus RIMD 2210633

39.709

100

0.412
  pilB Acinetobacter baumannii D1279779

38.855

100

0.409
  pilB Vibrio cholerae strain A1552

39.004

100

0.405
  pilB Acinetobacter baylyi ADP1

38.32

100

0.403
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.538

100

0.369

Multiple sequence alignment