Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   DV155_RS08435 Genome accession   NZ_CP031326
Coordinates   1424328..1426004 (+) Length   558 a.a.
NCBI ID   WP_002220273.1    Uniprot ID   -
Organism   Neisseria meningitidis strain M21374     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1419328..1431004
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DV155_RS08375 rpmB 1419472..1419705 (+) 234 WP_002216391.1 50S ribosomal protein L28 -
  DV155_RS08380 rpmG 1419737..1419892 (+) 156 WP_002212306.1 50S ribosomal protein L33 -
  DV155_RS08390 ubiM 1420163..1421347 (-) 1185 WP_002220268.1 5-demethoxyubiquinol-8 5-hydroxylase UbiM -
  DV155_RS08395 rpmA 1421582..1421854 (-) 273 WP_002212328.1 50S ribosomal protein L27 -
  DV155_RS08400 rplU 1421879..1422187 (-) 309 WP_002216394.1 50S ribosomal protein L21 -
  DV155_RS08410 ispB 1422411..1423385 (+) 975 WP_002220270.1 octaprenyl diphosphate synthase -
  DV155_RS08415 - 1423390..1423836 (+) 447 WP_002220271.1 DUF441 domain-containing protein -
  DV155_RS08435 pilF 1424328..1426004 (+) 1677 WP_002220273.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DV155_RS08440 - 1426089..1426298 (-) 210 WP_002212333.1 DNA gyrase inhibitor YacG -
  DV155_RS08445 coaE 1426291..1426923 (-) 633 WP_002220275.1 dephospho-CoA kinase -
  DV155_RS08450 pilD 1426925..1427785 (-) 861 WP_002220276.1 A24 family peptidase Machinery gene
  DV155_RS08455 pilG 1427859..1429091 (-) 1233 WP_002220278.1 type 4 pilus assembly protein PilG Machinery gene

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 61905.01 Da        Isoelectric Point: 5.3037

>NTDB_id=305703 DV155_RS08435 WP_002220273.1 1424328..1426004(+) (pilF) [Neisseria meningitidis strain M21374]
MSVGLLRILVQNQVVTVEQAEHYCNESQAGKEVLPMLFSDGVISPKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQ
MVEFHCVPVFRRGDKVFFAVSDPTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPIAVRGQLASRIKVMSRLDISEKR
IPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMRILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSG
KTVSLYTCLNILNTESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAAQ
TGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSSCKQEVERPSASALKEVGFTDEDLAKDWKLY
RAVGCDRCRGQGYKGRAGVYEVMPISEEMQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=305703 DV155_RS08435 WP_002220273.1 1424328..1426004(+) (pilF) [Neisseria meningitidis strain M21374]
ATGAGCGTAGGTTTGCTGAGGATTCTGGTTCAAAACCAGGTGGTTACTGTTGAGCAGGCCGAGCATTACTGCAATGAGTC
GCAGGCGGGTAAGGAAGTGTTGCCGATGCTGTTTTCAGACGGTGTCATTTCGCCCAAGTCGCTTGCGGCATTGATTGCGA
GGGTGTTCAGTTATTCGATTCTTGATTTGCGTCATTATCCGCGCCACAGGGTGCTGATGGGGGTGTTGACGGAGGAGCAG
ATGGTGGAGTTCCACTGTGTGCCGGTTTTCCGTCGGGGCGACAAAGTATTTTTTGCGGTTTCCGATCCGACACAGATGCC
GCAAATTCAGAAAACCGTTTCTGCCGCAGGGATTGAGGTTGAGTTGGTCATTGTCGAGGATGACCAGTTGGCGGGTTTGC
TCGATTGGGTGGGTTCGCGTTCGACATCGCTGCTTCAGGAGCTTGGGGAGGGGCAGGAGGAAGAGGAAAGCCACACCCTG
TATATCGACAACGAGGAGGCAGAAGACGGCCCTGTTCCGAGGTTTATCCATAAGACTTTGTCGGATGCCTTGCGCAGCGG
GGCATCGGACATCCATTTCGAGTTTTACGAACACAATGCCCGTATCCGTTTCCGTGTGGACGGGCAGCTCCGCGAGGTGG
TTCAGCCGCCCATTGCGGTAAGGGGGCAGCTTGCTTCCCGGATTAAGGTAATGTCGCGTTTGGACATTTCCGAAAAACGG
ATACCGCAGGACGGCAGGATGCAGCTGACCTTTCAAAAGGGCGGCAAGCCTGTCGATTTCCGTGTCAGCACATTGCCGAC
GCTGTTTGGCGAAAAGGTCGTGATGCGGATTTTGAATTCCGATGCCGCGTCTTTGAACATCGACCAGCTCGGTTTTGAGC
CGTTTCAGAAAAAATTGTTGTTGGAAGCGATTCACCGTCCCTACGGGATGGTGCTGGTAACCGGTCCGACGGGTTCGGGT
AAGACGGTGTCGCTCTATACCTGTTTGAATATTTTGAATACGGAGTCGGTAAATATTGCAACGGCGGAAGACCCTGCCGA
GATTAACCTGCCGGGCATCAATCAGGTTAACGTCAATGATAAGCAGGGTCTGACTTTTGCCGCTGCTTTGAAGTCTTTCC
TGCGTCAGGACCCGGACATCATTATGGTCGGTGAGATTCGTGATTTGGAAACTGCCGATATTGCGATTAAGGCGGCACAA
ACAGGGCATATGGTGTTTTCCACCCTGCACACCAATAATGCGCCGGCGACGTTGTCGCGTATGCTGAATATGGGTGTCGC
GCCGTTTAATATTGCCAGTTCGGTCAGCCTGATTATGGCGCAGCGTCTTTTACGCAGGCTGTGTTCGAGCTGCAAACAGG
AAGTGGAACGCCCGTCTGCCTCTGCTTTGAAGGAAGTCGGCTTCACCGATGAGGACCTTGCAAAAGATTGGAAACTTTAC
CGCGCCGTCGGTTGCGACCGTTGCCGGGGGCAGGGTTATAAGGGGCGTGCGGGCGTGTATGAGGTTATGCCCATCAGCGA
AGAAATGCAGCGTGTGATTATGAACAACGGTACGGAAGTGGATATTTTGGACGTTGCCTATAAGGAGGGTATGGTGGATT
TGCGCCGGGCCGGTATTTTGAAAGTTATGCAGGGCATTACTTCATTGGAAGAGGTAACGGCAAATACCAACGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(178-446)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

99.283

100

0.993
  pilB Acinetobacter baumannii D1279779

50.265

100

0.511
  pilB Acinetobacter baylyi ADP1

50.356

100

0.507
  pilB Legionella pneumophila strain ERS1305867

49.067

96.057

0.471
  pilB Vibrio parahaemolyticus RIMD 2210633

45.39

100

0.459
  pilB Vibrio cholerae strain A1552

45.374

100

0.457
  pilB Vibrio campbellii strain DS40M4

43.838

100

0.446
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.962

93.728

0.375
  pilF Thermus thermophilus HB27

36.738

100

0.367

Multiple sequence alignment