Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   DV115_RS07290 Genome accession   NZ_CP031325
Coordinates   1304050..1305726 (+) Length   558 a.a.
NCBI ID   WP_115437585.1    Uniprot ID   -
Organism   Neisseria polysaccharea strain M18661     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1299050..1310726
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DV115_RS07240 rpmB 1299268..1299501 (+) 234 WP_002216391.1 50S ribosomal protein L28 -
  DV115_RS07245 rpmG 1299533..1299688 (+) 156 WP_002212306.1 50S ribosomal protein L33 -
  DV115_RS12215 - 1299788..1299984 (-) 197 Protein_1227 hypothetical protein -
  DV115_RS07250 ubiM 1300039..1301223 (-) 1185 WP_025456643.1 5-demethoxyubiquinol-8 5-hydroxylase UbiM -
  DV115_RS07255 rpmA 1301458..1301730 (-) 273 WP_003689821.1 50S ribosomal protein L27 -
  DV115_RS07260 rplU 1301755..1302063 (-) 309 WP_002216394.1 50S ribosomal protein L21 -
  DV115_RS07270 ispB 1302287..1303261 (+) 975 WP_003750327.1 octaprenyl diphosphate synthase -
  DV115_RS07275 - 1303265..1303711 (+) 447 WP_003750326.1 DUF441 domain-containing protein -
  DV115_RS07290 pilF 1304050..1305726 (+) 1677 WP_115437585.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DV115_RS07295 - 1305811..1306020 (-) 210 WP_003710977.1 DNA gyrase inhibitor YacG -
  DV115_RS07300 coaE 1306013..1306645 (-) 633 WP_003750324.1 dephospho-CoA kinase -
  DV115_RS07305 pilD 1306647..1307507 (-) 861 WP_115437586.1 prepilin peptidase Machinery gene
  DV115_RS07310 pilG 1307581..1308813 (-) 1233 WP_003710974.1 type 4 pilus assembly protein PilG Machinery gene
  DV115_RS07315 - 1309055..1310374 (-) 1320 WP_025456642.1 MFS transporter -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 62101.22 Da        Isoelectric Point: 5.3060

>NTDB_id=305648 DV115_RS07290 WP_115437585.1 1304050..1305726(+) (pilF) [Neisseria polysaccharea strain M18661]
MSVGLLRILVQNQVVTVEQAEHYYKESQAGKEVLPILFSDGVISPKSLAALIARVFSYSVFDLRYYPRHRVLMGVLTEEQ
MVEFHCVPVFRRDNKVFFAVSDPTQMPQIQKTVSAAGIAVELVIVEDDQLAGLLDWVGSRSTSLLQELGEEQEEEESHTL
YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPIAVRGQLASRIKVMSRLDISEKR
IPQDGRMQLTFQKGGKPIDFRVSTLPTLFGEKVVMRILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSG
KTVSLYTCLNILNTESVNIATAEDPAEINLPGINQVNVNEKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAAQ
TGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSSCKQEVERPSASALKEIGFTDEDLAKDWKLY
HAVGCDRCRGQGYKGRAGVYEVMPISEEMQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=305648 DV115_RS07290 WP_115437585.1 1304050..1305726(+) (pilF) [Neisseria polysaccharea strain M18661]
ATGAGCGTAGGTTTGCTGAGGATTCTGGTTCAAAACCAGGTGGTTACTGTTGAGCAGGCCGAGCATTACTACAAGGAGTC
GCAGGCGGGTAAGGAAGTGTTGCCGATACTGTTTTCAGACGGCGTCATTTCGCCCAAGTCGCTTGCGGCATTGATTGCAA
GGGTGTTCAGTTATTCGGTTTTCGATTTGCGCTACTATCCGCGCCACAGGGTGCTGATGGGGGTGTTGACGGAGGAGCAG
ATGGTGGAGTTCCACTGTGTGCCGGTTTTCCGTCGGGACAATAAAGTATTTTTTGCGGTTTCCGATCCGACACAGATGCC
GCAAATTCAGAAAACCGTGTCTGCCGCAGGGATTGCGGTTGAGTTGGTCATTGTCGAGGATGACCAGTTGGCGGGTTTGC
TCGATTGGGTGGGGTCGAGATCGACATCGCTGCTTCAGGAGCTTGGGGAGGAGCAGGAGGAAGAGGAAAGCCACACCCTG
TATATCGACAATGAGGAGGCGGAGGACGGCCCTGTTCCGAGGTTTATCCATAAAACTTTGTCGGATGCCTTGCGCAGCGG
GGCATCGGACATTCATTTTGAGTTTTACGAACACAATGCCCGTATCCGTTTCCGCGTGGACGGGCAGCTTCGCGAGGTGG
TTCAGCCGCCTATTGCGGTAAGGGGGCAGCTTGCTTCCCGGATTAAGGTGATGTCGCGTTTGGATATTTCCGAAAAACGC
ATACCGCAGGACGGCAGGATGCAGCTGACCTTTCAAAAGGGCGGCAAGCCTATCGATTTCCGCGTCAGCACTTTGCCGAC
GCTGTTTGGCGAAAAGGTTGTGATGCGGATTTTGAACTCCGATGCCGCGTCTTTGAATATCGACCAGCTCGGTTTTGAGC
CGTTTCAGAAAAAATTGCTGTTGGAAGCGATTCATCGTCCTTACGGTATGGTGCTGGTAACCGGCCCGACGGGTTCGGGT
AAGACGGTGTCGCTCTATACCTGTTTGAATATTTTGAATACGGAGTCGGTAAATATTGCAACGGCGGAAGACCCTGCCGA
GATTAACCTGCCAGGCATCAATCAGGTCAACGTCAATGAGAAGCAGGGTCTGACTTTTGCCGCTGCTTTAAAGTCTTTCC
TGCGTCAGGATCCGGACATCATTATGGTCGGTGAGATTCGTGATTTGGAAACTGCCGATATTGCGATTAAGGCGGCACAA
ACAGGGCATATGGTGTTTTCCACCCTGCACACCAATAATGCGCCGGCGACGTTGTCGCGTATGCTGAATATGGGTGTCGC
GCCGTTTAATATTGCCAGTTCGGTCAGCCTGATTATGGCGCAGCGTCTTTTACGCAGGTTGTGTTCGAGCTGCAAACAGG
AAGTGGAACGCCCGTCTGCTTCTGCTCTGAAGGAAATCGGCTTCACCGATGAGGATCTTGCAAAAGATTGGAAACTTTAC
CATGCCGTCGGTTGCGACCGTTGCCGGGGTCAGGGTTACAAGGGGCGTGCGGGCGTGTATGAGGTTATGCCCATCAGCGA
AGAAATGCAGCGTGTGATTATGAACAACGGTACGGAAGTGGATATTTTGGACGTTGCCTATAAGGAGGGTATGGTGGATT
TGCGCCGGGCCGGTATTTTGAAAGTTATGCAGGGCATTACTTCATTGGAAGAGGTAACGGCAAATACCAACGATTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-139)

Type II/IV secretion system protein (T2SSE)

(178-446)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

97.491

100

0.975
  pilB Acinetobacter baumannii D1279779

50.532

100

0.511
  pilB Acinetobacter baylyi ADP1

50.178

100

0.505
  pilB Legionella pneumophila strain ERS1305867

47.527

100

0.482
  pilB Vibrio cholerae strain A1552

45.73

100

0.461
  pilB Vibrio parahaemolyticus RIMD 2210633

45.567

100

0.461
  pilB Vibrio campbellii strain DS40M4

44.149

100

0.446
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.993

96.057

0.375

Multiple sequence alignment