Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   EXA09_RS08860 Genome accession   NZ_CP035812
Coordinates   1877955..1878614 (-) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain B7A     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 1872955..1883614
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXA09_RS08835 parE 1873046..1874938 (+) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -
  EXA09_RS08840 ygiN 1874986..1875300 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  EXA09_RS08845 mdaB 1875331..1875912 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  EXA09_RS08850 ygiZ 1876231..1876563 (+) 333 WP_000912120.1 DUF2645 family protein -
  EXA09_RS08855 qseC 1876609..1877958 (-) 1350 WP_000673354.1 quorum sensing histidine kinase QseC -
  EXA09_RS08860 ciaR 1877955..1878614 (-) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  EXA09_RS08865 ygiW 1878766..1879158 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  EXA09_RS08870 ygiV 1879211..1879693 (+) 483 WP_000183494.1 GyrI-like domain-containing protein -
  EXA09_RS08875 parC 1880239..1882497 (+) 2259 WP_001281894.1 DNA topoisomerase IV subunit A -
  EXA09_RS08880 plsC 1882731..1883468 (+) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=305066 EXA09_RS08860 WP_001221502.1 1877955..1878614(-) (ciaR) [Escherichia coli strain B7A]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=305066 EXA09_RS08860 WP_001221502.1 1877955..1878614(-) (ciaR) [Escherichia coli strain B7A]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment