Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   DV114_RS00545 Genome accession   NZ_CP031251
Coordinates   99291..100970 (-) Length   559 a.a.
NCBI ID   WP_049331882.1    Uniprot ID   -
Organism   Neisseria subflava strain M18660     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 94291..105970
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DV114_RS00515 lspA 94646..95152 (-) 507 WP_167382871.1 signal peptidase II -
  DV114_RS00520 glyQ 95301..96200 (-) 900 WP_101756019.1 glycine--tRNA ligase subunit alpha -
  DV114_RS00525 yacG 96282..96464 (-) 183 WP_003682475.1 DNA gyrase inhibitor YacG -
  DV114_RS00530 coaE 96461..97081 (-) 621 WP_101756018.1 dephospho-CoA kinase -
  DV114_RS00535 pilD 97081..97968 (-) 888 WP_101756017.1 A24 family peptidase Machinery gene
  DV114_RS00540 pilG 97970..99211 (-) 1242 WP_101756016.1 type II secretion system F family protein Machinery gene
  DV114_RS00545 pilF 99291..100970 (-) 1680 WP_049331882.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DV114_RS00550 carB 101374..104589 (-) 3216 WP_114926050.1 carbamoyl-phosphate synthase large subunit -
  DV114_RS00555 - 104613..105419 (-) 807 WP_114926051.1 hypothetical protein -
  DV114_RS11190 - 105475..105657 (+) 183 WP_162817315.1 hypothetical protein -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62263.48 Da        Isoelectric Point: 6.0382

>NTDB_id=304967 DV114_RS00545 WP_049331882.1 99291..100970(-) (pilF) [Neisseria subflava strain M18660]
MSVGLLRVLVQSQTISNQQAEHYNNLLQSGKEILPNLFSDGIISPKALGELVARVFSYPLLDLHYYPRNNVVSDILTEEQ
MVQNRCIPIFKRGRKVYFAVSDPTQIQNFQKIAFASGLSVDLVVVPDDQLSSLLEWLGQRSTTILKEINDEHEATQQSQS
LYIDNEEAEDGPIPRFIHKTLSDALNAGASDIHFEFYEQMARVRFRVDGQLREVVQPPVAVRGQLASRIKVMARLDISEK
RIPQDGRIQIAFHKHGRPIDFRVSTLPTLFGEKVVMRILNSDGTSLNIDQLGLEPFQKEMLLEAINRPYGMVLVTGPTGS
GKTVSLYTCLSILNTEDVNISTAEDPAEINLPGVNQVNVNEKQGLTFAAALRSFLRQDPDIIMVGEIRDLETADIAIKAA
QTGHMVFSTLHTNNAPATLSRLLNMGVAPFNIASSVSLIMAQRLLRRLCPNCKREVERPPVPALKKAGFTDADLAQDWKL
YRPVGCDSCRGKGFKGRVGIYEVMPVSDEMQKVIMNNGTEVDIMNMAYQEGMVDLRRAGLMKVMQGLTSLEEVTAHTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=304967 DV114_RS00545 WP_049331882.1 99291..100970(-) (pilF) [Neisseria subflava strain M18660]
ATGAGTGTCGGATTATTGCGTGTTTTGGTTCAAAGCCAGACCATCTCAAATCAACAAGCGGAACATTACAACAACCTTTT
ACAATCAGGCAAAGAAATCCTGCCCAACCTGTTTTCAGACGGCATCATCTCCCCTAAAGCTTTGGGGGAGTTAGTTGCGC
GCGTATTCAGCTATCCCCTTTTGGATTTGCACTACTACCCGCGCAATAATGTAGTCAGCGACATTCTGACGGAAGAGCAA
ATGGTGCAAAACCGTTGCATTCCGATTTTCAAACGCGGACGCAAGGTTTATTTCGCCGTATCCGATCCGACCCAAATCCA
AAACTTTCAAAAAATCGCTTTTGCTTCAGGTTTAAGTGTCGACTTGGTAGTGGTTCCCGACGACCAGCTCAGCTCATTGC
TGGAATGGCTGGGCCAACGTTCGACCACCATTCTGAAAGAAATCAACGACGAACATGAAGCAACGCAGCAATCTCAATCG
CTTTATATCGATAACGAAGAAGCTGAAGACGGCCCTATCCCCCGTTTCATCCACAAAACCTTATCCGATGCCCTGAATGC
GGGTGCATCGGACATCCACTTTGAATTCTACGAACAAATGGCACGCGTCCGTTTCCGTGTGGACGGACAGTTGCGTGAAG
TCGTACAGCCGCCTGTCGCCGTGCGCGGCCAGTTGGCATCGCGCATCAAAGTGATGGCGCGTTTGGATATTTCCGAAAAA
CGTATTCCGCAAGACGGTAGAATTCAAATTGCCTTCCATAAACACGGCCGTCCGATTGACTTCCGTGTCAGTACTTTGCC
GACGCTGTTTGGTGAAAAAGTGGTGATGCGTATCCTAAATTCCGATGGTACATCGCTCAATATCGACCAGCTCGGTTTAG
AGCCTTTCCAAAAAGAAATGCTGTTGGAAGCCATCAACCGCCCTTACGGCATGGTATTGGTTACCGGTCCGACAGGTTCG
GGCAAAACCGTTTCTCTCTACACCTGTCTGAGTATTTTGAACACGGAAGATGTAAACATTTCGACGGCAGAAGATCCGGC
CGAGATTAACCTGCCGGGCGTCAACCAAGTCAACGTCAATGAAAAACAAGGTCTGACGTTTGCCGCCGCACTCCGATCCT
TCTTACGTCAAGATCCTGATATTATTATGGTCGGTGAGATTCGTGACTTGGAAACCGCCGACATCGCCATTAAAGCCGCC
CAAACCGGCCACATGGTTTTCTCTACCCTGCATACCAATAATGCACCGGCCACGCTGTCCCGCCTTTTGAACATGGGTGT
CGCGCCTTTCAACATCGCCAGCTCGGTCAGCCTAATTATGGCGCAAAGGTTGTTGCGCAGACTGTGCCCGAACTGCAAAC
GTGAAGTTGAGCGCCCGCCGGTTCCGGCATTGAAAAAAGCCGGCTTTACCGATGCGGACTTGGCTCAAGATTGGAAACTC
TACCGCCCGGTTGGCTGCGACAGCTGCCGCGGCAAAGGCTTTAAAGGCCGCGTCGGTATTTATGAAGTCATGCCGGTCAG
CGATGAAATGCAAAAAGTCATTATGAACAACGGTACAGAAGTTGATATCATGAACATGGCCTATCAGGAAGGCATGGTAG
ACTTGCGTCGTGCTGGCCTCATGAAAGTCATGCAAGGCCTGACCTCGTTGGAAGAAGTGACTGCTCATACCAATGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-139)

Type II/IV secretion system protein (T2SSE)

(179-447)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

77.639

100

0.776
  pilB Acinetobacter baylyi ADP1

51.056

100

0.519
  pilB Acinetobacter baumannii D1279779

50.619

100

0.512
  pilB Legionella pneumophila strain ERS1305867

47.173

100

0.478
  pilB Vibrio cholerae strain A1552

46.085

100

0.463
  pilB Vibrio campbellii strain DS40M4

44.306

100

0.445
  pilB Vibrio parahaemolyticus RIMD 2210633

46.542

95.707

0.445
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.364

98.39

0.377
  pilF Thermus thermophilus HB27

38.547

96.064

0.37

Multiple sequence alignment