Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoS   Type   Regulator
Locus tag   EW646_RS17380 Genome accession   NZ_CP035770
Coordinates   3478435..3479427 (-) Length   330 a.a.
NCBI ID   WP_000081550.1    Uniprot ID   A0A370V765
Organism   Escherichia coli O145 strain RM8988-C1     
Function   regulation of chitinases (predicted from homology)   
Competence regulation

Genomic Context

Location: 3473435..3484427
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW646_RS17355 (EW646_17835) pphB 3474798..3475454 (+) 657 WP_001141343.1 protein-serine/threonine phosphatase -
  EW646_RS17360 (EW646_17840) - 3475495..3475731 (-) 237 WP_000562979.1 non-oxidative hydroxyarylic acid decarboxylases subunit D -
  EW646_RS17365 (EW646_17845) - 3475742..3477169 (-) 1428 Protein_3445 non-oxidative hydroxyarylic acid decarboxylases subunit C -
  EW646_RS17370 (EW646_17850) - 3477169..3477762 (-) 594 WP_000767721.1 non-oxidative hydroxyarylic acid decarboxylases subunit B -
  EW646_RS17375 (EW646_17855) - 3477908..3478315 (+) 408 WP_001208062.1 MarR family transcriptional regulator -
  EW646_RS17380 (EW646_17860) rpoS 3478435..3479427 (-) 993 WP_000081550.1 RNA polymerase sigma factor RpoS Regulator
  EW646_RS17385 (EW646_17865) nlpD 3479490..3480584 (-) 1095 WP_306174120.1 murein hydrolase activator NlpD -
  EW646_RS17390 (EW646_17870) pcm 3480769..3481395 (-) 627 WP_000254708.1 protein-L-isoaspartate O-methyltransferase -
  EW646_RS17395 (EW646_17875) surE 3481389..3482150 (-) 762 WP_001295182.1 5'/3'-nucleotidase SurE -
  EW646_RS17400 (EW646_17880) truD 3482131..3483180 (-) 1050 WP_000568924.1 tRNA pseudouridine(13) synthase TruD -
  EW646_RS17405 (EW646_17885) ispF 3483177..3483656 (-) 480 WP_001219242.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  EW646_RS17410 (EW646_17890) ispD 3483656..3484366 (-) 711 WP_000246160.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -

Sequence

Protein


Download         Length: 330 a.a.        Molecular weight: 37972.84 Da        Isoelectric Point: 4.5597

>NTDB_id=304824 EW646_RS17380 WP_000081550.1 3478435..3479427(-) (rpoS) [Escherichia coli O145 strain RM8988-C1]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide


Download         Length: 993 bp        

>NTDB_id=304824 EW646_RS17380 WP_000081550.1 3478435..3479427(-) (rpoS) [Escherichia coli O145 strain RM8988-C1]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAATAA

Domains

Predicted by InterProScan.

Sigma-70 region 3 (Sigma70_r3)

(174-248)

Sigma-70 region 2 (Sigma70_r2)

(94-163)

Sigma-70 factor, region 1.2 (Sigma70_r1_2)

(56-88)

Sigma-70, region 4 (Sigma70_r4)

(262-315)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A370V765

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoS Vibrio cholerae O1 biovar El Tor strain E7946

73.065

97.879

0.715

Multiple sequence alignment