Detailed information    

insolico Bioinformatically predicted

Overview

Name   crp   Type   Regulator
Locus tag   EWS90_RS16545 Genome accession   NZ_CP035739
Coordinates   3387999..3388643 (+) Length   214 a.a.
NCBI ID   WP_003085214.1    Uniprot ID   A0A0H2ZLY6
Organism   Pseudomonas aeruginosa strain 1334/14     
Function   promote expression of competence genes (predicted from homology)   
Competence regulation

Genomic Context

Location: 3382999..3393643
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EWS90_RS16520 (EWS90_16520) - 3383492..3384973 (-) 1482 WP_003121853.1 AAA family ATPase -
  EWS90_RS16525 (EWS90_16525) - 3385052..3385390 (+) 339 WP_003085225.1 histidine triad nucleotide-binding protein -
  EWS90_RS16530 (EWS90_16530) coq7 3385490..3386137 (+) 648 WP_003085224.1 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone monooxygenase -
  EWS90_RS16535 (EWS90_16535) speD 3386192..3386986 (-) 795 WP_003085223.1 adenosylmethionine decarboxylase -
  EWS90_RS16540 (EWS90_16540) - 3387305..3387727 (-) 423 WP_003085219.1 OsmC family protein -
  EWS90_RS16545 (EWS90_16545) crp 3387999..3388643 (+) 645 WP_003085214.1 cAMP-activated global transcriptional regulator CRP Regulator
  EWS90_RS16550 (EWS90_16550) trpC 3388705..3389541 (-) 837 WP_016561543.1 indole-3-glycerol phosphate synthase TrpC -
  EWS90_RS16555 (EWS90_16555) trpD 3389538..3390587 (-) 1050 WP_003085203.1 anthranilate phosphoribosyltransferase -
  EWS90_RS16560 (EWS90_16560) - 3390589..3391194 (-) 606 WP_023085654.1 aminodeoxychorismate/anthranilate synthase component II -
  EWS90_RS16565 (EWS90_16565) - 3391542..3391799 (-) 258 WP_031800321.1 hypothetical protein -
  EWS90_RS16570 (EWS90_16570) - 3391796..3392158 (-) 363 WP_004355109.1 hypothetical protein -
  EWS90_RS16575 (EWS90_16575) - 3392155..3392784 (-) 630 WP_003113192.1 glycoside hydrolase family 19 protein -

Sequence

Protein


Download         Length: 214 a.a.        Molecular weight: 24225.01 Da        Isoelectric Point: 8.0525

>NTDB_id=304490 EWS90_RS16545 WP_003085214.1 3387999..3388643(+) (crp) [Pseudomonas aeruginosa strain 1334/14]
MVAITHTPKLKHLDKLLAHCHRRRYTAKSTIIYAGDRCETLFFIIKGSVTILIEDDDGREMIIGYLNSGDFFGELGLFEK
EGSEQERSAWVRAKVECEVAEISYAKFRELSQQDSEILYTLGSQMADRLRKTTRKVGDLAFLDVTGRVARTLLDLCQQPD
AMTHPDGMQIKITRQEIGRIVGCSREMVGRVLKSLEEQGLVHVKGKTMVVFGTR

Nucleotide


Download         Length: 645 bp        

>NTDB_id=304490 EWS90_RS16545 WP_003085214.1 3387999..3388643(+) (crp) [Pseudomonas aeruginosa strain 1334/14]
ATGGTAGCTATTACCCACACACCCAAACTCAAACACCTAGACAAGCTGCTCGCACACTGTCACCGCCGCCGCTACACCGC
AAAGAGCACCATCATCTATGCCGGCGATCGCTGCGAAACGCTGTTCTTCATCATCAAGGGTTCGGTCACCATCCTCATCG
AGGACGACGACGGCCGCGAAATGATCATCGGCTACCTCAACAGCGGTGATTTCTTCGGCGAGCTGGGATTGTTCGAAAAG
GAAGGCAGCGAACAGGAACGCAGCGCCTGGGTTCGTGCCAAGGTGGAATGCGAAGTCGCCGAGATCAGCTATGCGAAGTT
CCGCGAACTGTCGCAGCAGGACTCGGAGATCCTCTACACCCTCGGCAGCCAGATGGCCGACCGCCTGCGCAAGACCACCC
GCAAGGTGGGCGACCTGGCCTTCCTCGACGTCACCGGACGCGTCGCCCGCACCTTGCTGGACCTGTGCCAGCAACCGGAC
GCCATGACCCACCCGGACGGCATGCAGATCAAGATCACCCGCCAGGAGATCGGCCGGATCGTCGGCTGCTCGCGGGAAAT
GGTCGGACGCGTGCTGAAGAGCCTGGAGGAGCAGGGCCTGGTGCATGTGAAAGGAAAGACCATGGTGGTCTTCGGCACCC
GCTGA

Domains

Predicted by InterProScan.

Cyclic nucleotide-binding domain (cNMP_binding)

(22-114)

Crp-like helix-turn-helix domain (HTH_Crp_2)

(146-209)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZLY6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  crp Vibrio cholerae strain A1552

67.327

94.393

0.636
  crp Haemophilus influenzae Rd KW20

60.396

94.393

0.57
  crp Acinetobacter baumannii D1279779

54.545

92.523

0.505

Multiple sequence alignment