Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   DV400_RS01400 Genome accession   NZ_CP031236
Coordinates   273592..274041 (-) Length   149 a.a.
NCBI ID   WP_005691824.1    Uniprot ID   A0A2S9R750
Organism   Haemophilus influenzae strain M25267     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 268592..279041
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DV400_RS01380 rho 268979..270241 (+) 1263 WP_005629550.1 transcription termination factor Rho -
  DV400_RS01385 pilD 270295..270987 (-) 693 WP_050948983.1 A24 family peptidase Machinery gene
  DV400_RS01390 pilC 270984..272204 (-) 1221 WP_050948984.1 type II secretion system F family protein Machinery gene
  DV400_RS01395 pilB 272201..273595 (-) 1395 WP_005691826.1 GspE/PulE family protein Machinery gene
  DV400_RS01400 pilA 273592..274041 (-) 450 WP_005691824.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  DV400_RS01405 ampD 274156..274710 (+) 555 WP_005691822.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  DV400_RS01410 corC 275342..276241 (+) 900 WP_050948985.1 CNNM family magnesium/cobalt transport protein CorC -
  DV400_RS01415 lnt 276264..277793 (+) 1530 WP_076773244.1 apolipoprotein N-acyltransferase -
  DV400_RS01420 rsmE 277843..278580 (+) 738 WP_005691816.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15409.89 Da        Isoelectric Point: 9.3908

>NTDB_id=304325 DV400_RS01400 WP_005691824.1 273592..274041(-) (pilA) [Haemophilus influenzae strain M25267]
MKLTTLQTLKKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAVSELLQASAPYKADVELCVYSTGKPSTCSGGSNGIA
ADITTAKGYVASVKTQSGGITVKGNGTLANMEYILQAKGNATAGVTWTTTCKGTDASLFPANFCGSVTK

Nucleotide


Download         Length: 450 bp        

>NTDB_id=304325 DV400_RS01400 WP_005691824.1 273592..274041(-) (pilA) [Haemophilus influenzae strain M25267]
ATGAAATTAACAACACTGCAAACCTTGAAAAAAGGGTTTACATTAATCGAGCTAATGATTGTGATTGCAATTATTGCTAT
TTTAGCCACTATCGCAATTCCTTCTTATCAAAATTATACCAAAAAAGCTGCGGTATCCGAATTACTGCAAGCTTCTGCGC
CTTATAAGGCTGATGTGGAATTATGCGTTTATAGCACAGGCAAACCTTCTACTTGCTCAGGAGGAAGCAATGGAATTGCA
GCTGATATTACAACAGCAAAAGGCTATGTAGCCTCAGTGAAAACTCAATCAGGTGGTATTACAGTAAAAGGGAATGGCAC
ATTGGCAAATATGGAATATATTTTGCAAGCTAAAGGTAATGCTACAGCAGGTGTAACTTGGACAACAACCTGCAAAGGAA
CGGATGCCTCTTTATTTCCAGCAAATTTTTGCGGAAGTGTCACAAAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S9R750

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae Rd KW20

93.289

100

0.933
  pilA Haemophilus influenzae 86-028NP

87.919

100

0.879
  pilA Glaesserella parasuis strain SC1401

57.047

100

0.57
  pilA2 Legionella pneumophila strain ERS1305867

39.189

99.329

0.389
  pilA Vibrio campbellii strain DS40M4

39.716

94.631

0.376

Multiple sequence alignment