Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HYN46_RS16760 Genome accession   NZ_CP031222
Coordinates   3714416..3716125 (+) Length   569 a.a.
NCBI ID   WP_114900449.1    Uniprot ID   -
Organism   Aquirhabdus parva strain HYN0046     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3709416..3721125
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HYN46_RS16725 (HYN46_16725) nusA 3709886..3711361 (-) 1476 WP_114900444.1 transcription termination factor NusA -
  HYN46_RS16730 (HYN46_16730) - 3711507..3712103 (-) 597 WP_114900445.1 ribosome assembly cofactor RimP -
  HYN46_RS16745 (HYN46_16745) secG 3712709..3713047 (-) 339 WP_114900446.1 preprotein translocase subunit SecG -
  HYN46_RS16750 (HYN46_16750) tpiA 3713064..3713870 (-) 807 WP_114900447.1 triose-phosphate isomerase -
  HYN46_RS16755 (HYN46_16755) - 3713982..3714290 (+) 309 WP_114900448.1 DUF1653 domain-containing protein -
  HYN46_RS16760 (HYN46_16760) pilB 3714416..3716125 (+) 1710 WP_114900449.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HYN46_RS16765 (HYN46_16765) pilC 3716290..3717516 (+) 1227 WP_114900450.1 type II secretion system F family protein Machinery gene
  HYN46_RS16770 (HYN46_16770) pilD 3717525..3718412 (+) 888 WP_407640809.1 prepilin peptidase Machinery gene
  HYN46_RS16775 (HYN46_16775) coaE 3718562..3719170 (+) 609 WP_114900452.1 dephospho-CoA kinase -
  HYN46_RS16780 (HYN46_16780) - 3719210..3720085 (+) 876 WP_114900453.1 PhzF family phenazine biosynthesis protein -
  HYN46_RS16785 (HYN46_16785) - 3720128..3720238 (+) 111 Protein_3298 4-carboxymuconolactone decarboxylase -
  HYN46_RS16790 (HYN46_16790) - 3720331..3720585 (-) 255 WP_114900454.1 pyocin activator PrtN family protein -
  HYN46_RS16795 (HYN46_16795) - 3720585..3720833 (-) 249 WP_114900455.1 hypothetical protein -
  HYN46_RS16800 (HYN46_16800) - 3720820..3721062 (-) 243 WP_114900456.1 hypothetical protein -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62450.93 Da        Isoelectric Point: 5.2504

>NTDB_id=304178 HYN46_RS16760 WP_114900449.1 3714416..3716125(+) (pilB) [Aquirhabdus parva strain HYN0046]
MSGQAVSFGGLARQIVNEGIVTAEIMQRAASDAQTEKITLISHLVSKKLAKADQIAWLVARDFGDSLFDLDAIDQSLIPK
DLVDEKIVRQFSVVPIFKRGSRIFVAMSDPTRVDALEAIRFASRLAVEAVVVEEDKLARLIEKLYENMTTADFSGFGGDV
ELDVETENNDDKNANDAAGAEEAPVVKFINKMLIDAIRMGASDLHFEPYEKSYRIRYRVDGVMREIARPPIQLAPKLAAR
LKVMSQMDISEKRMPQDGRIKLKVSKTKAIDFRVNSLPTLYGEKIVLRILDASSAMLGIDALGYEPEQKALFMAALERPQ
GMLLITGPTGSGKTVSLYTGLNILNTVDTNISTAEDPVEINLEGINQVNVNLKTGLTFAVALRAFLRQDPDIVMVGEIRD
LETAEIAVKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPACKSPVDVPAQSLKEMGFT
DEDLATGFKVYQPVGCSECRDGYKGRIGIYEVMKVTSEISRIIMEEGNAIDIADASRRSGFNDLRRSGLIKVMQGMTSLQ
EVNRVTGGE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=304178 HYN46_RS16760 WP_114900449.1 3714416..3716125(+) (pilB) [Aquirhabdus parva strain HYN0046]
ATGTCTGGACAAGCGGTATCCTTCGGTGGTTTAGCGCGTCAGATTGTGAATGAAGGTATCGTCACAGCTGAAATTATGCA
GCGCGCAGCCAGCGATGCTCAAACCGAAAAAATCACATTAATCTCGCATTTAGTCTCCAAAAAATTGGCCAAAGCCGATC
AGATTGCTTGGTTGGTTGCGCGCGATTTTGGTGATTCCTTATTTGATCTTGATGCGATAGATCAGAGCCTCATCCCGAAA
GATCTTGTAGACGAGAAGATTGTTCGTCAGTTTTCTGTTGTGCCTATTTTTAAGCGCGGTTCCCGAATATTTGTCGCGAT
GAGCGATCCGACACGGGTGGATGCGTTGGAAGCCATCCGTTTTGCCAGCCGCTTAGCGGTTGAGGCGGTTGTGGTCGAAG
AGGACAAGCTGGCGCGACTGATAGAAAAGCTTTATGAAAACATGACCACCGCTGATTTTAGCGGCTTTGGTGGTGATGTT
GAGCTGGATGTCGAAACCGAAAACAACGACGATAAAAATGCAAACGATGCAGCAGGAGCGGAAGAAGCTCCTGTTGTAAA
GTTCATTAATAAAATGCTGATTGATGCGATTCGTATGGGGGCTTCAGACTTACATTTTGAGCCCTATGAGAAAAGCTATC
GGATTCGCTATCGTGTTGATGGTGTGATGCGCGAAATTGCCCGTCCGCCTATTCAATTGGCGCCTAAATTGGCAGCGCGT
TTGAAAGTCATGTCGCAAATGGACATTTCTGAAAAGCGTATGCCACAAGACGGTCGAATTAAGCTCAAAGTTTCTAAAAC
CAAAGCAATTGACTTTCGTGTGAACTCTCTGCCAACTCTGTATGGCGAAAAAATTGTTCTGCGTATTTTGGATGCGTCGA
GTGCGATGCTGGGTATTGATGCGCTCGGTTATGAGCCTGAACAAAAAGCATTGTTTATGGCTGCTCTTGAGCGCCCACAG
GGAATGTTGTTGATCACAGGTCCAACTGGTTCAGGGAAAACGGTTTCGCTCTATACGGGTCTGAATATCTTGAATACAGT
TGATACGAATATTTCAACAGCAGAAGACCCTGTTGAAATTAACTTAGAAGGGATTAATCAGGTTAATGTCAACTTAAAAA
CAGGCTTAACCTTTGCGGTCGCGCTCCGTGCATTCTTACGCCAAGATCCTGATATTGTGATGGTGGGTGAAATCCGTGAC
CTTGAAACAGCCGAAATAGCTGTAAAAGCTGCACAAACAGGGCATATGGTGATGTCAACATTGCATACCAACAGTGCTCC
GGAGACGTTAACGCGTCTGCGTAACATGGGTGTGCCTTCATTTAATATTGCAACTTCAGTGAATCTGGTGATTGCGCAGC
GCTTAGCACGCAGATTGTGCCCAGCGTGCAAGAGCCCTGTTGATGTACCGGCGCAAAGTTTAAAAGAAATGGGCTTTACC
GATGAGGATTTGGCAACAGGATTTAAAGTTTATCAGCCCGTTGGCTGCTCTGAATGTCGTGATGGCTATAAAGGCCGTAT
TGGAATTTACGAGGTAATGAAAGTCACGTCTGAGATTTCACGGATTATTATGGAAGAAGGCAATGCGATCGATATTGCCG
ATGCATCGCGTCGATCGGGCTTCAATGATTTACGCCGTTCAGGACTGATCAAAGTTATGCAAGGTATGACCAGTCTGCAA
GAGGTAAACCGAGTCACAGGTGGCGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

68.384

98.946

0.677
  pilB Acinetobacter baylyi ADP1

66.548

98.77

0.657
  pilF Neisseria gonorrhoeae MS11

53.058

97.715

0.518
  pilB Legionella pneumophila strain ERS1305867

53.618

94.728

0.508
  pilB Vibrio parahaemolyticus RIMD 2210633

47.74

97.188

0.464
  pilB Vibrio cholerae strain A1552

49.905

92.97

0.464
  pilB Vibrio campbellii strain DS40M4

46.751

97.364

0.455
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.873

88.576

0.362

Multiple sequence alignment