Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DCD74_RS08070 Genome accession   NZ_CP029556
Coordinates   1667719..1669449 (+) Length   576 a.a.
NCBI ID   WP_112926858.1    Uniprot ID   A0A344J6I5
Organism   Solilutibacter oculi strain 83-4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1662719..1674449
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DCD74_RS08050 (DCD74_08050) - 1663411..1665030 (+) 1620 WP_112926854.1 PAS domain-containing sensor histidine kinase -
  DCD74_RS08055 (DCD74_08055) pilR 1665062..1666519 (+) 1458 WP_112926855.1 sigma-54 dependent transcriptional regulator Regulator
  DCD74_RS13205 (DCD74_08060) - 1666749..1667114 (+) 366 WP_112926856.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  DCD74_RS13130 (DCD74_08065) - 1667329..1667658 (+) 330 WP_112926857.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  DCD74_RS08070 (DCD74_08070) pilB 1667719..1669449 (+) 1731 WP_112926858.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DCD74_RS08075 (DCD74_08075) pilC 1669522..1670727 (+) 1206 WP_407072233.1 type II secretion system F family protein Machinery gene
  DCD74_RS08080 (DCD74_08080) - 1670731..1671597 (+) 867 WP_112926860.1 A24 family peptidase -
  DCD74_RS08085 (DCD74_08085) coaE 1671602..1672222 (+) 621 WP_112926861.1 dephospho-CoA kinase -
  DCD74_RS08090 (DCD74_08090) - 1672223..1673194 (-) 972 WP_112926862.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62698.11 Da        Isoelectric Point: 6.0491

>NTDB_id=293489 DCD74_RS08070 WP_112926858.1 1667719..1669449(+) (pilB) [Solilutibacter oculi strain 83-4]
MNAQPNTQNLVGITGITRRLAMDGALEIDGARAAQEAAAKARIPIAQWLISEKLVTPAQLTAANAMEFGLSIFDPGAMPP
HPETLKLVSEELIQKHKVLPLFKRGNRLFVGTADPANLQGMDEIKFQSSLAVEPVLVDIDTLQRTLEIWQATGDSLGDLL
GGDDDLNTLDVAEDHADGGDQGVDAKGDDDTPVVKFINKILIDAIRRGASDIHFEPYEESYRVRLRIDGVLRSVTRMANK
MHTRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQMDFRVSTLPTLFGEKIVLRILDGGAAKLGIEKLGYEEAQQKLF
LDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDDTRNISTVEDPVEIRLPGVNQVQQNTKTGMTFAAALRSFLRQDPDV
IMVGEIRDLETAEIGVKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPFNIISSVTLVIAQRLARRLCPSCKREAKLPEH
ALLAEGFTHEEIAAGLKVYEASGCSECTEGYKGRTGIYQVMPMTEEIQAIVLEGGNAMQIAAAAQRSGVNDLRQSAMLKV
KQGVTSLTEINRVTKD

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=293489 DCD74_RS08070 WP_112926858.1 1667719..1669449(+) (pilB) [Solilutibacter oculi strain 83-4]
ATGAACGCACAGCCGAATACCCAAAATCTGGTCGGTATCACCGGCATTACCCGTCGCTTGGCGATGGATGGCGCATTGGA
GATCGATGGCGCGCGAGCAGCGCAGGAGGCCGCGGCCAAGGCCCGCATCCCCATCGCCCAATGGCTGATCAGCGAGAAGC
TGGTCACCCCCGCCCAACTGACCGCCGCGAATGCGATGGAATTCGGACTGTCAATCTTCGACCCCGGTGCAATGCCCCCT
CACCCGGAAACCCTCAAGCTGGTTTCCGAAGAGCTGATCCAGAAGCACAAGGTGCTGCCGCTATTCAAGCGCGGCAACCG
GCTCTTTGTGGGCACCGCGGATCCCGCCAACCTGCAGGGCATGGATGAGATCAAGTTCCAGTCCAGCCTGGCAGTCGAAC
CGGTGTTGGTGGACATCGATACCCTGCAGCGCACGCTGGAAATCTGGCAGGCCACGGGCGACTCGCTGGGTGACTTGCTC
GGTGGCGACGACGACCTGAACACGCTCGATGTCGCCGAAGATCACGCGGATGGTGGCGATCAGGGCGTCGATGCCAAGGG
TGATGACGACACGCCCGTGGTCAAGTTCATCAACAAGATTCTGATTGATGCCATCCGCCGGGGCGCTTCTGACATCCACT
TCGAACCCTATGAAGAGAGCTACCGCGTGCGCCTGCGCATCGACGGCGTACTGCGCAGTGTTACCCGCATGGCCAACAAG
ATGCATACCCGCATCGCCGCCCGCCTCAAGGTCATGGCCCAGCTCGATATCGCCGAAAAGCGCGTCCCTCAGGACGGTCG
CATCAAGCTGAACCTGTCGAAGACCAAGCAGATGGACTTCCGCGTCAGCACCCTGCCCACCTTGTTTGGCGAAAAGATCG
TGCTGCGTATCCTGGATGGCGGTGCGGCGAAGCTGGGCATCGAGAAGCTGGGCTATGAGGAGGCACAGCAGAAACTCTTC
CTCGACGCCATCCACAAGCCCTATGGGATGGTGCTGGTCACCGGCCCGACCGGCTCCGGCAAGACCGTGTCGCTGTACAC
GGCACTGGGCATCCTCAACGACGACACCCGCAACATCTCGACGGTCGAAGACCCGGTGGAAATCCGCCTGCCCGGCGTCA
ACCAGGTCCAGCAGAACACCAAGACCGGCATGACCTTCGCCGCCGCGCTGCGCTCTTTCCTGCGACAGGATCCGGATGTG
ATCATGGTCGGCGAAATCCGCGACCTGGAGACTGCCGAGATCGGCGTCAAGGCCGCCCAGACAGGTCACATGGTGCTGTC
CACCCTGCATACCAATGACGCGCCGCAGACCATTGCCCGCCTGATGAACATGGGCATCGCACCGTTCAACATCATCAGTT
CGGTGACCCTGGTCATCGCCCAGCGCCTGGCCCGTCGGCTCTGCCCATCCTGCAAGCGCGAGGCCAAGCTGCCCGAACAC
GCCCTGCTGGCCGAGGGCTTTACCCACGAGGAAATCGCGGCCGGGCTCAAGGTCTACGAGGCCAGCGGTTGCAGTGAATG
CACGGAAGGGTATAAAGGGCGCACTGGCATCTACCAGGTCATGCCGATGACCGAAGAGATCCAGGCCATCGTGTTGGAAG
GGGGCAATGCCATGCAGATCGCCGCGGCCGCGCAACGGTCCGGCGTGAATGACCTGCGCCAGTCGGCGATGCTCAAGGTC
AAGCAGGGCGTGACCAGCCTTACGGAAATCAACCGCGTCACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-149)

Type II/IV secretion system protein (T2SSE)

(199-466)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A344J6I5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.379

98.438

0.545
  pilB Acinetobacter baumannii D1279779

54.561

98.958

0.54
  pilB Legionella pneumophila strain ERS1305867

49.209

98.785

0.486
  pilF Neisseria gonorrhoeae MS11

48.501

98.438

0.477
  pilB Vibrio cholerae strain A1552

47.909

99.653

0.477
  pilB Vibrio campbellii strain DS40M4

47.276

98.785

0.467
  pilB Vibrio parahaemolyticus RIMD 2210633

47.67

96.875

0.462
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.394

91.667

0.361

Multiple sequence alignment