Detailed information    

insolico Bioinformatically predicted

Overview

Name   clpE   Type   Regulator
Locus tag   EQI87_RS07865 Genome accession   NZ_CP035166
Coordinates   1454669..1456768 (-) Length   699 a.a.
NCBI ID   WP_046380982.1    Uniprot ID   -
Organism   Bacillus subtilis strain SRCM103971     
Function   repress competence development (at the early growth phase) (predicted from homology)   
Competence regulation

Genomic Context

Location: 1449669..1461768
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQI87_RS07830 (EQI87_07830) spo0E 1449724..1449981 (+) 258 WP_003218598.1 aspartyl-phosphate phosphatase Spo0E -
  EQI87_RS07835 (EQI87_07835) - 1450067..1450498 (+) 432 WP_029726990.1 hypothetical protein -
  EQI87_RS07840 (EQI87_07840) kinD 1450526..1452046 (-) 1521 WP_015252216.1 sporulation kinase KinD -
  EQI87_RS07845 (EQI87_07845) mhqR 1452239..1452676 (+) 438 WP_003232475.1 MarR family transcriptional regulator MhqR -
  EQI87_RS07850 (EQI87_07850) motB 1452716..1453501 (-) 786 WP_003232473.1 flagellar motor protein MotB -
  EQI87_RS07855 (EQI87_07855) motA 1453473..1454285 (-) 813 WP_003238984.1 flagellar motor stator protein MotA -
  EQI87_RS07865 (EQI87_07865) clpE 1454669..1456768 (-) 2100 WP_046380982.1 ATP-dependent protease ATP-binding subunit ClpE Regulator
  EQI87_RS07870 (EQI87_07870) - 1457134..1458177 (+) 1044 WP_015715790.1 membrane protein -
  EQI87_RS07875 (EQI87_07875) queC 1458491..1459150 (+) 660 WP_015715791.1 7-cyano-7-deazaguanine synthase QueC -
  EQI87_RS07880 (EQI87_07880) queD 1459143..1459592 (+) 450 WP_003232462.1 6-carboxytetrahydropterin synthase QueD -
  EQI87_RS07885 (EQI87_07885) queE 1459585..1460316 (+) 732 WP_003232460.1 7-carboxy-7-deazaguanine synthase QueE -
  EQI87_RS07890 (EQI87_07890) queF 1460334..1460831 (+) 498 WP_003218613.1 preQ(1) synthase -
  EQI87_RS07895 (EQI87_07895) ykvN 1461308..1461664 (-) 357 WP_003245808.1 winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 699 a.a.        Molecular weight: 77787.85 Da        Isoelectric Point: 5.1837

>NTDB_id=293302 EQI87_RS07865 WP_046380982.1 1454669..1456768(-) (clpE) [Bacillus subtilis strain SRCM103971]
MRCQHCHQNEATIRLNMQINSVHKQMVLCETCYNELTRKPSMSMGPQSFGFPFEQAFQPKEKSAAKQSGKKGLLDELAQN
ITNGAKAGLIDPVIGRDDEVARVIEILNRRNKNNPVLIGEPGVGKTAIAEGLALKIAEGDVPNKLKNKELYLLDVASLVA
NTGIRGQFEERMKQLITELKERKNVILFIDEIHLLVGAGSAEGSMDAGNILKPALARGELQVIGATTLKEYRQIEKDAAL
ERRFQPVVVQEPSIEQAILILQGIKDKYEAYHGVTFSDEAIKACVTLSSRYIQDRHLPDKAIDLLDEAGSKANLLIDELN
DEDAAERLTAIEAEKTKALEDENYELAAKLRDEELALEKKLNSSSAHTAVTVEAEHIQEIVEQKTGIPVGKLQADEQTKM
KELEAKLHERVIGQEAAVQKVAKAVRRSRAGLKSKNRPVGSFLFVGPTGVGKTELSKTLADELFGTKDAIIRLDMSEYME
KHAVSKIIGSPPGYVGHEEAGQLTEKVRRNPYSIVLLDEIEKAHPDVQHMFLQIMEDGRLTDSQGRTVSFKDTVIIMTSN
AGAGEKQTKVGFQSDDSVIEEQTLIDSLSMFFKPEFLNRFDSIIEFRSLEKEHLVKIVSLLLGELEETLAERGISLNVTD
EAKEKIAELGYHPSFGARPLRRTIQEWVEDEMTDLLLDNGEITSFHVILEDDKIKVRAK

Nucleotide


Download         Length: 2100 bp        

>NTDB_id=293302 EQI87_RS07865 WP_046380982.1 1454669..1456768(-) (clpE) [Bacillus subtilis strain SRCM103971]
ATGCGTTGTCAACATTGTCATCAAAACGAGGCGACGATTCGCCTTAACATGCAAATAAATTCCGTTCATAAACAGATGGT
TCTTTGTGAAACTTGCTATAACGAACTGACCCGTAAACCTTCAATGAGTATGGGTCCTCAATCTTTCGGATTTCCGTTTG
AACAGGCATTCCAGCCGAAAGAAAAGAGCGCAGCAAAACAAAGCGGAAAAAAAGGGTTGCTTGATGAGTTGGCTCAAAAT
ATTACAAACGGTGCTAAAGCCGGTCTCATTGATCCCGTCATCGGCCGTGATGATGAAGTGGCGCGAGTGATCGAAATTCT
AAACCGCCGCAACAAAAACAATCCGGTTCTTATTGGTGAGCCGGGTGTGGGGAAAACTGCCATCGCTGAAGGGCTCGCTT
TAAAAATTGCTGAAGGCGATGTTCCAAACAAACTGAAAAACAAAGAGCTATACTTGCTTGATGTTGCATCCCTTGTTGCA
AATACAGGGATCAGAGGCCAATTTGAGGAGAGAATGAAACAGCTGATCACTGAGCTGAAGGAACGAAAAAATGTCATTCT
GTTCATTGATGAAATTCACCTTCTCGTCGGCGCAGGCTCTGCAGAAGGATCAATGGACGCCGGCAACATTCTCAAACCGG
CCCTAGCCAGAGGCGAACTGCAAGTCATTGGCGCGACAACACTGAAAGAATATCGTCAAATCGAAAAAGATGCCGCGCTG
GAAAGACGTTTTCAGCCTGTCGTGGTGCAGGAGCCTTCAATTGAACAGGCTATCCTCATTCTGCAAGGGATTAAAGACAA
ATACGAGGCGTACCATGGCGTAACATTCAGTGATGAAGCAATCAAAGCGTGTGTCACTTTATCATCCCGCTACATTCAGG
ACAGACACCTGCCGGATAAAGCAATTGATTTATTAGATGAAGCAGGTTCAAAAGCCAACCTGTTAATTGATGAACTGAAT
GATGAGGATGCCGCTGAACGCTTAACTGCAATTGAAGCCGAAAAAACAAAAGCCCTGGAAGATGAAAATTACGAACTGGC
GGCAAAACTCCGTGATGAAGAACTCGCATTGGAGAAAAAACTGAACAGCTCCTCCGCTCATACCGCTGTCACTGTGGAAG
CTGAGCACATTCAGGAAATTGTTGAACAAAAAACAGGCATCCCTGTCGGCAAACTGCAGGCAGACGAACAAACGAAAATG
AAAGAACTCGAAGCAAAACTTCATGAACGCGTGATTGGACAAGAAGCCGCTGTTCAAAAAGTGGCAAAGGCGGTAAGACG
AAGCCGCGCCGGTTTAAAATCCAAAAACAGACCAGTCGGCTCCTTCCTCTTCGTCGGTCCTACCGGCGTAGGGAAAACAG
AGCTTTCTAAAACACTGGCAGATGAATTATTCGGCACAAAAGACGCTATTATCCGACTCGATATGAGCGAATACATGGAG
AAACACGCCGTATCTAAAATTATCGGTTCACCGCCTGGATATGTCGGCCATGAGGAAGCTGGACAATTAACTGAGAAAGT
GCGCCGCAATCCTTACAGCATTGTGTTGCTGGATGAGATTGAAAAAGCACACCCAGACGTTCAGCATATGTTCCTGCAAA
TTATGGAAGATGGCCGTCTGACAGACAGCCAAGGCAGAACCGTAAGCTTCAAAGACACTGTGATCATCATGACAAGTAAT
GCGGGTGCTGGTGAGAAACAAACGAAAGTCGGTTTCCAATCAGATGACAGTGTCATCGAAGAACAAACATTAATTGATTC
ACTGAGCATGTTCTTTAAACCTGAGTTCCTCAACCGTTTTGACAGCATTATTGAGTTCCGCTCATTGGAAAAAGAACATC
TTGTCAAAATCGTCAGCCTTCTTCTTGGAGAACTTGAAGAAACATTGGCGGAACGGGGCATTAGCTTGAATGTGACAGAT
GAAGCGAAAGAAAAAATCGCTGAGCTGGGCTACCACCCTTCATTCGGTGCACGTCCGCTTAGAAGAACCATCCAAGAATG
GGTTGAGGATGAAATGACCGATCTGCTGCTTGATAATGGCGAGATCACAAGTTTTCACGTGATTTTAGAAGATGATAAAA
TCAAAGTGCGAGCGAAATAA

Domains

Predicted by InterProScan.

AAA domain (Cdc48 subfamily) (AAA_2)

(437-603)

AAA lid domain (AAA_lid_9)

(253-352)

ATPase family associated with various cellular activities (AAA) (AAA)

(116-247)

C-terminal, D2-small domain, of ClpB protein (ClpB_D2-small)

(609-679)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  clpE Streptococcus mutans UA159

56.662

100

0.602
  clpC Lactococcus lactis subsp. cremoris KW2

56.284

100

0.589
  clpE Streptococcus pneumoniae TIGR4

54.785

100

0.565
  clpE Streptococcus pneumoniae Rx1

54.785

100

0.565
  clpE Streptococcus pneumoniae D39

54.785

100

0.565
  clpE Streptococcus pneumoniae R6

54.785

100

0.565
  clpC Bacillus subtilis subsp. subtilis str. 168

54.016

90.844

0.491
  clpC Streptococcus pneumoniae Rx1

45.884

93.848

0.431
  clpC Streptococcus pneumoniae D39

45.884

93.848

0.431
  clpC Lactococcus lactis subsp. lactis strain DGCC12653

47.687

89.7

0.428
  clpC Streptococcus thermophilus LMD-9

45.427

93.848

0.426
  clpC Streptococcus thermophilus LMG 18311

44.97

93.848

0.422
  clpC Streptococcus pneumoniae TIGR4

46.457

90.844

0.422
  clpC Streptococcus mutans UA159

46.216

88.841

0.411
  clpA Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

41.749

86.695

0.362

Multiple sequence alignment