Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   SALB1_RS13760 Genome accession   NZ_CP029488
Coordinates   3067137..3068867 (-) Length   576 a.a.
NCBI ID   WP_109994378.1    Uniprot ID   -
Organism   Salinisphaera sp. LB1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3062137..3073867
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SALB1_RS13740 (SALB1_2803) rpmF 3062348..3062533 (+) 186 WP_109994374.1 50S ribosomal protein L32 -
  SALB1_RS13745 (SALB1_2804) plsX 3062666..3063688 (+) 1023 WP_179950669.1 phosphate acyltransferase PlsX -
  SALB1_RS13750 (SALB1_2805) clpS 3063938..3064267 (+) 330 WP_109994376.1 ATP-dependent Clp protease adapter ClpS -
  SALB1_RS13755 (SALB1_2806) clpA 3064328..3066595 (+) 2268 WP_109994377.1 ATP-dependent Clp protease ATP-binding subunit ClpA -
  SALB1_RS13760 (SALB1_2807) pilB 3067137..3068867 (-) 1731 WP_109994378.1 type IV-A pilus assembly ATPase PilB Machinery gene
  SALB1_RS13765 (SALB1_2808) - 3069019..3069468 (+) 450 WP_109994379.1 hemerythrin domain-containing protein -
  SALB1_RS13775 (SALB1_2809) - 3069691..3070659 (-) 969 WP_109994380.1 polyprenyl synthetase family protein -
  SALB1_RS13780 (SALB1_2810) rplU 3070821..3071135 (+) 315 WP_109995450.1 50S ribosomal protein L21 -
  SALB1_RS13785 (SALB1_2811) rpmA 3071177..3071440 (+) 264 WP_109994381.1 50S ribosomal protein L27 -
  SALB1_RS13790 (SALB1_2812) cgtA 3071549..3072619 (+) 1071 WP_109994382.1 Obg family GTPase CgtA -
  SALB1_RS13795 (SALB1_2813) proB 3072606..3073739 (+) 1134 WP_109994383.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62847.68 Da        Isoelectric Point: 5.3094

>NTDB_id=293131 SALB1_RS13760 WP_109994378.1 3067137..3068867(-) (pilB) [Salinisphaera sp. LB1]
MPTTQRTTRQTTLGGLARQLVEDGLIEYEAALRIQAEAQRNEESFVARAVATRALSAPVLARALARAFSMPLLDLRAVDL
AHAPIDAAPESLIRKHGALPIFRRGRTLYLAVADPTNLQALDEFRFNTGQQIEAVLVEQDKLERAIESNFSALDSAMAGL
DDVALDNLAVEQDGGDSTSSDALKADADDAPVVRFVNKVLLDAINRGASDIHFEPYEQRYRVRQRQDGVLREVASPPAAI
GMRLSARLKVMARLDLAERRIPQDGRIKLNLSKNRSIDFRVSTCPTLHGEKIVLRILDPASAQMGIEALGYEDYQREIFE
STIHKPYGMVLVTGPTGSGKTVSLYTALNILNTADRNISTAEDPAEINLPGVNQVNVQPRVGLTFAACLRAFLRQDPDVI
MVGEIRDLETAEIAIKAAQTGHMVLSTVHTNDAPQTLTRLMNMGVPAYNIAASVTLIIAQRLARRLCPQCKTAAEIPPDA
LIEEGFSVEEIEHLTIYEANLKGCDHCTDGYRGRVGIYQVMPVTDAIGQIIMSGGNADDIEKQAASEGVLNLRQAGLNKV
RHGVISLAELNRVTVE

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=293131 SALB1_RS13760 WP_109994378.1 3067137..3068867(-) (pilB) [Salinisphaera sp. LB1]
ATGCCGACAACCCAACGCACGACGCGCCAGACGACCCTTGGCGGCCTGGCGCGACAACTCGTCGAAGACGGCCTCATCGA
GTACGAGGCGGCGCTTCGTATCCAGGCGGAAGCCCAGCGCAACGAAGAGTCGTTCGTGGCGCGGGCGGTGGCGACCCGGG
CGCTTAGTGCGCCGGTCCTGGCACGGGCCCTGGCGCGCGCTTTCTCCATGCCGCTGCTGGATCTGCGCGCGGTGGATCTC
GCCCATGCCCCGATCGACGCCGCCCCCGAGAGCCTGATCCGCAAGCACGGGGCGCTGCCCATTTTCCGGCGCGGCCGGAC
GCTGTATCTGGCGGTGGCGGATCCCACCAATCTGCAGGCGCTGGACGAATTCCGTTTCAATACCGGCCAGCAGATCGAAG
CGGTACTGGTCGAGCAGGACAAGCTCGAACGCGCGATCGAATCGAACTTCAGCGCGCTCGACAGCGCGATGGCCGGCCTG
GACGATGTCGCGCTCGACAATCTCGCAGTCGAGCAGGATGGCGGCGACAGCACCAGCTCCGATGCGCTCAAGGCCGACGC
CGATGATGCGCCCGTGGTGCGTTTCGTGAACAAAGTATTGCTGGACGCCATCAACCGCGGCGCATCGGACATCCACTTCG
AACCCTACGAACAACGCTACCGGGTGCGCCAGCGCCAGGACGGCGTGCTGCGCGAAGTGGCCTCGCCCCCGGCCGCGATC
GGCATGCGCCTGTCGGCGCGGCTGAAAGTCATGGCACGGCTGGATTTGGCCGAACGACGCATCCCGCAGGACGGGCGTAT
CAAGCTCAACCTGTCGAAGAACCGATCCATTGATTTCCGCGTCTCGACCTGCCCGACACTGCACGGCGAGAAGATCGTGT
TGCGTATCCTCGATCCAGCCAGCGCACAGATGGGAATTGAAGCGCTCGGTTACGAGGATTACCAACGCGAAATCTTCGAG
TCGACCATTCACAAGCCCTACGGCATGGTGCTGGTGACCGGCCCCACCGGCAGCGGCAAGACGGTGTCGCTCTATACCGC
GCTCAATATCCTCAACACGGCGGACCGCAACATTTCCACCGCCGAGGATCCGGCGGAAATCAACCTGCCGGGCGTGAACC
AGGTCAACGTACAGCCACGCGTCGGGCTGACCTTCGCCGCCTGCCTGCGGGCGTTCCTGCGCCAGGATCCGGACGTCATC
ATGGTCGGCGAGATCCGCGACCTGGAGACGGCGGAAATTGCCATCAAGGCCGCCCAGACCGGGCATATGGTGTTGTCGAC
GGTGCACACGAATGACGCGCCGCAGACCCTCACCCGTCTGATGAACATGGGCGTGCCCGCCTACAATATCGCCGCCTCGG
TCACGCTGATCATCGCCCAGCGCCTGGCGCGCCGGCTCTGCCCCCAGTGCAAGACCGCAGCAGAAATCCCGCCCGATGCG
CTGATCGAGGAAGGCTTCTCGGTCGAGGAAATCGAGCATCTGACGATCTATGAAGCCAACCTGAAAGGTTGCGATCATTG
TACCGACGGCTATCGCGGACGCGTGGGGATCTATCAGGTGATGCCGGTCACCGATGCTATCGGCCAGATCATCATGTCGG
GCGGCAATGCAGACGACATCGAGAAACAGGCCGCCTCGGAGGGCGTGCTCAACCTTCGCCAGGCCGGCCTCAACAAGGTG
CGCCACGGGGTCATCAGCCTGGCCGAACTCAACCGCGTCACGGTGGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(53-151)

Type II/IV secretion system protein (T2SSE)

(198-465)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

53.391

99.826

0.533
  pilB Acinetobacter baylyi ADP1

53.887

98.264

0.53
  pilB Acinetobacter baumannii D1279779

54.093

97.569

0.528
  pilF Neisseria gonorrhoeae MS11

48.264

100

0.483
  pilB Vibrio cholerae strain A1552

52.233

89.41

0.467
  pilB Vibrio campbellii strain DS40M4

51.262

89.41

0.458
  pilB Vibrio parahaemolyticus RIMD 2210633

49.81

91.319

0.455
  pilB/pilB1 Synechocystis sp. PCC 6803

38.368

100

0.384
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.775

92.535

0.368
  pilF Thermus thermophilus HB27

38.662

93.403

0.361

Multiple sequence alignment