Detailed information NTG0292958

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: B7L90_RS16615
• Genome accession: NZ_CP029473
• Coordinates: 3299401..3300015 (+)
• Length: 204 a.a.
• NCBI ID: WP_003152867.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain Hx05
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3294401..3305015

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
B7L90_RS16580 (B7L90_11570)	aroE	3295088..3295930 (+)	843	WP_025852995.1	shikimate dehydrogenase	-
B7L90_RS16585 (B7L90_11565)	yhbY	3295924..3296214 (+)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
B7L90_RS16590 (B7L90_11560)	-	3296225..3296794 (+)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
B7L90_RS16595 (B7L90_11555)	yqeK	3296784..3297365 (+)	582	WP_003152863.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
B7L90_RS16600 (B7L90_11550)	rsfS	3297362..3297718 (+)	357	WP_003152864.1	ribosome silencing factor	-
B7L90_RS16605 (B7L90_11545)	-	3297715..3298452 (+)	738	WP_025852989.1	class I SAM-dependent methyltransferase	-
B7L90_RS16610 (B7L90_11540)	comER	3298521..3299342 (-)	822	WP_003152866.1	late competence protein ComER	-
B7L90_RS16615 (B7L90_11535)	comEA	3299401..3300015 (+)	615	WP_003152867.1	helix-hairpin-helix domain-containing protein	Machinery gene
B7L90_RS16620 (B7L90_11530)	-	3300082..3300651 (+)	570	WP_003152868.1	ComE operon protein 2	-
B7L90_RS16625 (B7L90_11525)	comEC	3300652..3303003 (+)	2352	WP_025852986.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
B7L90_RS16630 (B7L90_11520)	-	3303022..3303156 (-)	135	WP_003152870.1	YqzM family protein	-
B7L90_RS16635	-	3303197..3303351 (+)	155	Protein_3176	hypothetical protein	-
B7L90_RS16640 (B7L90_11515)	holA	3303391..3304432 (+)	1042	Protein_3177	DNA polymerase III subunit delta	-
B7L90_RS16645 (B7L90_11510)	rpsT	3304449..3304715 (-)	267	WP_003152876.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22269.48 Da
• Isoelectric Point: 6.4940

>NTDB_id=292958 B7L90_RS16615 WP_003152867.1 3299401..3300015(+) (comEA) [Bacillus velezensis strain Hx05]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=292958 B7L90_RS16615 WP_003152867.1 3299401..3300015(+) (comEA) [Bacillus velezensis strain Hx05]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGAAGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363