Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   DIR46_RS08780 Genome accession   NZ_CP029343
Coordinates   1903168..1904895 (+) Length   575 a.a.
NCBI ID   WP_109344900.1    Uniprot ID   A0A2S2DGQ7
Organism   Massilia oculi strain CCUG 43427     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1898168..1909895
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DIR46_RS08760 (DIR46_08760) - 1898900..1899457 (+) 558 WP_109344896.1 ClpP family protease -
  DIR46_RS08765 (DIR46_08765) - 1899649..1900890 (+) 1242 WP_109344897.1 hypothetical protein -
  DIR46_RS08770 (DIR46_08770) - 1900901..1901305 (+) 405 WP_109344898.1 hypothetical protein -
  DIR46_RS08775 (DIR46_08775) - 1901397..1902965 (+) 1569 WP_109344899.1 methyl-accepting chemotaxis protein -
  DIR46_RS08780 (DIR46_08780) pilF 1903168..1904895 (+) 1728 WP_109344900.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DIR46_RS08785 (DIR46_08785) pilC 1904917..1906149 (+) 1233 WP_109344901.1 type II secretion system F family protein Machinery gene
  DIR46_RS08790 (DIR46_08790) - 1906228..1907598 (-) 1371 WP_109344902.1 hypothetical protein -
  DIR46_RS08795 (DIR46_08795) - 1907696..1909801 (-) 2106 WP_109344903.1 glycoside hydrolase family 36 protein -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62086.48 Da        Isoelectric Point: 5.0939

>NTDB_id=291787 DIR46_RS08780 WP_109344900.1 1903168..1904895(+) (pilF) [Massilia oculi strain CCUG 43427]
MAAVLPNTTTGTQLSGLARALVQANRLSAAQADMLHKKAVQEKTAFIDAVLASGAIEPRALAAFCAETFGYPLLDLSAFA
PEFLPTSAIDARLMQAQRVIALAKRGNKLSVALSDPTNTQALDQIKFQSEATVEPVIVPHDALLTLLAAIAKGAEQDLSE
LAGDDAEIEFAEEDQQSATNPEAASDVEDAPIVRFLNKILMDAVQMGASDIHFEPYEKYYRIRLRVDGVLRDHAMPPLSI
REKLVSRIKVLARLDIAEKRVPQDGRMRLILSATRTIDFRVSTLPTLFGEKTVMRILDATQAQMGIDALGYDPDQKALLL
DAITRPYGMVLVTGPTGSGKTVSLYSCLNLLNKPGINISTAEDPAEINLPGVNQVNVNEKAGLTFPVALKSFLRQDPDII
MVGEIRDLETADIAIKAAQTGHMVFSTLHTNDAPSTLTRLMNMGVAPFNIASSVILITAQRLARRLCGCKQPLEISREAL
LAAGYRDSDLDGDWRPYGPIGCDRCLGSGYKGRVGIYQIMPISPSIEALILANGNSMEIAAQAEKEGVNSLRRSGLLKVK
QGLTSLEEVLGCTNE

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=291787 DIR46_RS08780 WP_109344900.1 1903168..1904895(+) (pilF) [Massilia oculi strain CCUG 43427]
ATGGCAGCAGTCCTCCCCAACACGACCACCGGTACGCAACTGTCGGGCCTGGCGCGCGCGCTGGTACAGGCCAATCGCCT
GAGCGCCGCGCAGGCGGACATGCTGCACAAGAAGGCGGTCCAGGAGAAGACCGCCTTCATCGACGCCGTGCTCGCCAGCG
GCGCGATCGAGCCGCGCGCGCTGGCCGCCTTCTGCGCCGAGACCTTCGGCTATCCGCTGCTCGACCTGTCGGCCTTCGCG
CCGGAATTCCTGCCCACCAGCGCCATCGACGCCAGGCTGATGCAGGCCCAGCGCGTGATCGCGCTGGCCAAGCGCGGCAA
CAAGCTGTCGGTCGCGCTGTCCGACCCGACCAATACCCAGGCCCTGGACCAGATCAAGTTCCAGAGCGAGGCGACGGTCG
AGCCGGTGATCGTGCCGCACGATGCGCTGCTGACCCTGCTGGCCGCGATCGCCAAGGGCGCCGAACAGGATCTGAGCGAG
CTGGCCGGCGACGACGCCGAGATCGAATTCGCCGAGGAAGACCAGCAGTCGGCCACGAACCCGGAAGCCGCCAGCGACGT
CGAGGACGCGCCCATCGTGCGCTTCCTGAACAAGATCCTGATGGACGCGGTACAGATGGGCGCGTCGGACATCCACTTCG
AGCCCTACGAAAAGTATTACCGGATCCGCCTGCGGGTCGACGGCGTGCTGCGCGACCATGCGATGCCGCCGCTCTCGATC
CGCGAAAAACTGGTCTCGCGCATCAAGGTGCTGGCGCGGCTGGACATCGCCGAAAAGCGCGTGCCGCAGGACGGCCGCAT
GCGGCTGATCCTGTCGGCCACGCGCACCATCGATTTCCGTGTCAGCACCCTGCCCACGCTGTTCGGCGAAAAAACGGTGA
TGCGTATCCTGGACGCGACCCAGGCGCAAATGGGCATCGACGCGCTCGGCTACGACCCGGACCAGAAGGCGCTGCTGCTG
GACGCGATCACCCGTCCCTACGGCATGGTGCTGGTGACGGGCCCGACCGGTTCGGGCAAGACGGTGTCGCTGTACAGCTG
CCTGAATCTGCTGAACAAGCCAGGGATCAATATTTCGACCGCGGAAGACCCTGCCGAGATCAACCTGCCGGGCGTGAACC
AGGTCAACGTCAACGAAAAGGCCGGCCTGACCTTCCCGGTGGCGCTGAAATCCTTCCTGCGCCAGGATCCCGACATCATC
ATGGTGGGCGAGATCCGCGACCTGGAAACGGCCGACATCGCGATCAAGGCCGCCCAGACCGGCCACATGGTGTTCTCGAC
CCTGCACACCAACGATGCGCCGTCGACCCTGACGCGCCTGATGAACATGGGCGTGGCGCCCTTCAATATCGCCTCGTCGG
TGATCCTGATCACGGCCCAGCGCCTGGCGCGCCGCCTGTGCGGCTGCAAGCAGCCGCTCGAGATCAGCCGCGAGGCCCTG
CTGGCCGCCGGCTACCGCGACAGCGACCTGGACGGCGACTGGCGGCCCTATGGCCCGATCGGCTGCGACCGCTGCCTGGG
TTCGGGCTACAAGGGGCGCGTCGGCATCTACCAGATCATGCCGATCTCCCCCAGTATCGAGGCGCTGATCCTGGCCAACG
GCAATTCCATGGAGATCGCGGCACAGGCCGAGAAGGAAGGCGTCAACTCCTTGCGCCGCTCGGGCCTGCTGAAAGTGAAA
CAGGGGCTGACCAGCCTGGAAGAAGTGCTTGGCTGCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(198-465)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(53-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S2DGQ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

54.529

97.913

0.534
  pilB Acinetobacter baylyi ADP1

53.603

98.957

0.53
  pilB Acinetobacter baumannii D1279779

53.345

98.783

0.527
  pilB Legionella pneumophila strain ERS1305867

48.496

98.261

0.477
  pilB Vibrio parahaemolyticus RIMD 2210633

46.346

97.565

0.452
  pilB Vibrio cholerae strain A1552

45.826

97.913

0.449
  pilB Vibrio campbellii strain DS40M4

45.115

97.913

0.442
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.132

92.174

0.379
  pilF Thermus thermophilus HB27

40.262

92.87

0.374

Multiple sequence alignment