Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DEH84_RS13785 Genome accession   NZ_CP029210
Coordinates   3074526..3076250 (+) Length   574 a.a.
NCBI ID   WP_109037371.1    Uniprot ID   A0A2U8FTK1
Organism   Aquabacterium olei strain NBRC 110486     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3069526..3081250
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DEH84_RS13765 (DEH84_13765) - 3070102..3070944 (+) 843 WP_109037367.1 DUF707 domain-containing protein -
  DEH84_RS13770 (DEH84_13770) - 3070941..3071615 (+) 675 WP_109037368.1 DUF4254 domain-containing protein -
  DEH84_RS13775 (DEH84_13775) waaC 3071631..3072605 (+) 975 WP_109037369.1 lipopolysaccharide heptosyltransferase I -
  DEH84_RS13780 (DEH84_13780) - 3072631..3074361 (+) 1731 WP_109037370.1 ABC transporter transmembrane domain-containing protein -
  DEH84_RS13785 (DEH84_13785) pilB 3074526..3076250 (+) 1725 WP_109037371.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DEH84_RS13790 (DEH84_13790) pilC 3076288..3077511 (+) 1224 WP_109037372.1 type II secretion system F family protein Machinery gene
  DEH84_RS13795 (DEH84_13795) - 3077519..3078394 (+) 876 WP_109037373.1 A24 family peptidase -
  DEH84_RS13800 (DEH84_13800) coaE 3078394..3079014 (+) 621 WP_170119677.1 dephospho-CoA kinase -
  DEH84_RS13805 (DEH84_13805) zapD 3079033..3079791 (+) 759 WP_109037374.1 cell division protein ZapD -
  DEH84_RS13810 (DEH84_13810) - 3079817..3080035 (+) 219 WP_109037375.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62499.85 Da        Isoelectric Point: 5.3585

>NTDB_id=291243 DEH84_RS13785 WP_109037371.1 3074526..3076250(+) (pilB) [Aquabacterium olei strain NBRC 110486]
MADSLLADAPGSLSGVARVLVNAGKLDARAAETLTRTAKEQKRSFVSTLVQSGVIEPGELAHTLSKALAVPVMDLAALDV
ERLPKGVIDKKLSAQYQVLVLSKRGNRLFVAGADPTDQEAIERIKFATQLTPEWVIVEQDKLAKLLDGLTASAEEQLSAL
ASSDFEFDSTEDVGAPQPDTNDVASEVEDAPVVRFLQKMLIDAINARASDLHFEPYEYNYRVRFRVDGELREVTQPPVAI
KDKLASRIKVISKLDIAEKRVPQDGRMKLKFGSKAIDFRVSTLPTLFGEKIVIRILDPSSAKLGIEALGYEAIEKERLLN
AIRRPYGMVLVTGPTGSGKTVSLYTCLNILNQPGVNISTVEDPAEINLPGINQVNVNDKAGLTFSAALKAFLRQDPDVIM
VGEIRDLETADIAIKAAQTGHMVMSTLHTNDAPTTLTRLMNMGVPPFNIASSVILITAQRLARRLCDNCKVPADIPRQAL
LDAGYKEEDLDGTWTPYRAVGCSACNNGYKGRLGIYQVMPISEEIQRIILNQGTAMDIAKQAKREGVRDLRESGLVKVKA
GLTSLEEVISVTNE

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=291243 DEH84_RS13785 WP_109037371.1 3074526..3076250(+) (pilB) [Aquabacterium olei strain NBRC 110486]
ATGGCCGATTCCCTTCTCGCTGATGCCCCTGGCTCGCTGTCCGGCGTGGCCCGCGTGCTCGTCAACGCAGGCAAGCTGGA
TGCACGCGCGGCCGAAACCCTCACGCGCACCGCCAAGGAGCAAAAGCGCAGCTTCGTCAGCACGCTGGTCCAGAGCGGCG
TCATCGAACCCGGCGAACTCGCCCACACGCTGTCGAAGGCGCTGGCCGTGCCGGTGATGGACCTGGCCGCGCTCGACGTC
GAACGCCTGCCCAAGGGCGTCATCGACAAGAAGCTCTCGGCCCAGTATCAGGTGCTGGTGCTCTCCAAGCGGGGCAACCG
GCTGTTCGTGGCCGGCGCCGACCCGACCGACCAGGAAGCGATCGAGCGCATCAAGTTCGCCACGCAGCTCACGCCCGAAT
GGGTCATCGTCGAGCAGGACAAGCTCGCGAAGCTGCTTGACGGCCTCACGGCCAGCGCGGAAGAGCAGCTCTCGGCCCTG
GCCTCCAGCGACTTCGAATTCGACTCCACCGAAGACGTGGGCGCCCCCCAGCCCGACACGAACGACGTCGCCTCCGAGGT
GGAAGACGCCCCGGTGGTGCGCTTCCTCCAGAAGATGCTGATCGACGCGATCAACGCGCGCGCCTCCGACCTGCACTTCG
AGCCCTACGAATACAACTACCGCGTGCGCTTCCGCGTCGACGGCGAGCTGCGCGAGGTCACCCAGCCGCCAGTCGCCATC
AAGGACAAGCTCGCGTCGCGCATCAAGGTCATCTCCAAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGACGGCCGCAT
GAAGCTCAAGTTCGGCTCCAAGGCCATCGACTTCCGCGTCAGCACCCTGCCCACGCTGTTCGGCGAAAAGATCGTGATCC
GTATCCTCGACCCGTCCAGCGCCAAGCTGGGCATCGAGGCCCTGGGTTACGAGGCGATCGAGAAAGAGCGCCTGCTCAAC
GCCATCAGGCGCCCGTACGGCATGGTGCTGGTCACCGGCCCGACCGGCTCCGGCAAGACGGTGTCGCTCTACACCTGCCT
GAACATCCTGAACCAGCCGGGCGTGAACATCTCGACGGTCGAAGACCCGGCGGAAATCAACCTGCCCGGCATCAACCAGG
TCAACGTCAACGACAAGGCCGGCCTCACCTTCTCGGCCGCGCTGAAGGCCTTCCTGCGCCAGGATCCCGACGTGATCATG
GTCGGTGAAATCCGTGACCTCGAAACCGCCGACATCGCCATCAAGGCCGCCCAGACCGGCCACATGGTGATGTCCACCCT
GCACACGAACGACGCGCCCACCACGCTGACGCGGTTGATGAACATGGGCGTGCCGCCCTTCAACATCGCTTCCAGCGTGA
TCCTGATCACCGCACAGCGACTGGCGCGCCGGCTGTGCGACAACTGCAAGGTCCCCGCCGACATTCCGAGGCAGGCCCTG
CTGGACGCCGGTTACAAGGAAGAAGACCTCGACGGCACCTGGACACCGTATCGCGCAGTGGGTTGCTCAGCTTGCAACAA
CGGTTACAAAGGTCGGCTGGGCATTTACCAAGTCATGCCCATCAGTGAGGAAATACAACGCATCATCCTGAATCAAGGTA
CTGCGATGGACATCGCCAAGCAGGCCAAGCGGGAAGGCGTGCGAGACTTGCGCGAATCGGGCCTGGTCAAGGTCAAGGCT
GGCCTCACCTCACTCGAAGAGGTGATTTCGGTGACCAATGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-155)

Type II/IV secretion system protein (T2SSE)

(198-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2U8FTK1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.497

99.826

0.554
  pilF Neisseria gonorrhoeae MS11

54.291

99.477

0.54
  pilB Acinetobacter baylyi ADP1

53.484

100

0.535
  pilB Legionella pneumophila strain ERS1305867

50.35

99.652

0.502
  pilB Vibrio parahaemolyticus RIMD 2210633

47.509

97.909

0.465
  pilB Vibrio campbellii strain DS40M4

45.975

97.387

0.448
  pilB Vibrio cholerae strain A1552

47.566

93.031

0.443
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.454

92.16

0.373
  pilF Thermus thermophilus HB27

39.252

93.206

0.366

Multiple sequence alignment