Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   CRG85_RS22875 Genome accession   NZ_CP034787
Coordinates   4729036..4730556 (+) Length   506 a.a.
NCBI ID   WP_000058987.1    Uniprot ID   -
Organism   Escherichia coli strain ECCNB20-2     
Function   require for natural transformation (predicted from homology)   
Unclear

Genomic Context

Location: 4724036..4735556
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CRG85_RS22850 (CRG85_22845) ilvE 4725590..4726519 (-) 930 WP_000208515.1 branched-chain-amino-acid transaminase -
  CRG85_RS22855 (CRG85_22850) ilvM 4726539..4726802 (-) 264 WP_000983255.1 acetolactate synthase 2 small subunit -
  CRG85_RS22860 (CRG85_22855) ilvG 4726799..4728445 (-) 1647 WP_001012619.1 acetolactate synthase 2 catalytic subunit -
  CRG85_RS22865 (CRG85_22860) ilvX 4728448..4728498 (-) 51 WP_001387183.1 peptide IlvX -
  CRG85_RS22870 (CRG85_22865) ilvL 4728585..4728683 (-) 99 WP_001311244.1 ilv operon leader peptide -
  CRG85_RS22875 (CRG85_22870) comM 4729036..4730556 (+) 1521 WP_000058987.1 YifB family Mg chelatase-like AAA ATPase Machinery gene
  CRG85_RS22880 (CRG85_22875) maoP 4730581..4730919 (-) 339 WP_000841001.1 macrodomain Ori organization protein MaoP -
  CRG85_RS22885 (CRG85_22880) hdfR 4731038..4731877 (+) 840 WP_000379246.1 HTH-type transcriptional regulator HdfR -

Sequence

Protein


Download         Length: 506 a.a.        Molecular weight: 55261.42 Da        Isoelectric Point: 7.6190

>NTDB_id=290802 CRG85_RS22875 WP_000058987.1 4729036..4730556(+) (comM) [Escherichia coli strain ECCNB20-2]
MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKEARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGR
YDLPIAIALLAASEQLTANKLDEYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
QAVCAFLEGKHTLERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGPPGTGKTMLASRINGLLPDLSNE
EALESAAILSLVNAESVQKQWRQRPFRSPHHSASLTAMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIES
GQIHLSRTRAKITYPARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGILSKTVVPG
ESSTTVKQRVMAARERQFKRQNKLNAWLDSPEIRKFCKLESEDAQWLEETLIHLGLSIRAWQRLLKVARTIADIDQSDII
TRQHLQEAVSYRAIDRLLIHLQKLLT

Nucleotide


Download         Length: 1521 bp        

>NTDB_id=290802 CRG85_RS22875 WP_000058987.1 4729036..4730556(+) (comM) [Escherichia coli strain ECCNB20-2]
ATGTCACTGTCAATTGTTCATACCCGCGCAGCCCTGGGAGTAAATGCGCCCCCAATCACTGTTGAGGTACATATCAGTAA
AGGTCTACCCGGTTTAACGATGGTGGGCTTACCAGAAACAACGGTAAAAGAAGCCCGCGATCGCGTGCGCAGCGCCATTA
TCAATAGCGGATATGAATATCCGGCCAAAAAAATCACCATCAACCTGGCTCCAGCCGATCTGCCAAAAGAAGGGGGACGA
TATGATTTACCTATCGCCATTGCGTTGCTGGCGGCCTCAGAACAGCTTACAGCCAATAAGTTAGATGAATATGAATTAGT
CGGAGAACTGGCGCTTACAGGCGCTCTGCGTGGCGTTCCCGGTGCAATCTCCAGTGCAACTGAAGCTATTAAGTCGGGCA
GAAAAATTATCGTCGCGAAAGATAACGAAGATGAAGTGGGGCTAATTAACGGTGAAGGATGCCTGATAGCCGATCATCTG
CAGGCTGTCTGTGCGTTTCTGGAAGGTAAGCACACTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACA
ACATGATCTCAGTGATGTTGTCGGTCAGGAGCAAGGAAAGCGAGGACTGGAAATTACCGCCGCTGGTGGGCACAACCTTT
TACTGATTGGACCGCCGGGAACAGGTAAAACAATGCTCGCCAGCCGTATTAATGGCCTGTTGCCAGATTTAAGCAATGAA
GAGGCACTGGAGAGCGCTGCGATATTAAGTCTGGTAAATGCTGAATCAGTACAAAAACAATGGCGGCAGCGCCCGTTCCG
CTCACCTCATCACAGTGCGTCGTTAACTGCGATGGTGGGCGGTGGTGCAATTCCAGGGCCAGGTGAAATTTCGCTGGCGC
ATAACGGCGTGCTTTTTCTTGATGAGTTACCTGAGTTTGAACGGCGTACACTGGATGCCTTGCGAGAGCCGATTGAATCC
GGGCAGATCCATCTTTCACGCACACGAGCAAAAATAACCTATCCAGCCCGTTTCCAGCTTGTCGCGGCGATGAATCCCAG
CCCTACCGGACATTATCAGGGAAACCATAACCGCTGCACGCCAGAACAGACATTACGTTATCTCAACCGGCTCTCGGGGC
CCTTTCTCGACCGCTTCGATCTCTCACTGGAGATCCCATTACCACCCCCCGGCATTTTGAGTAAAACGGTAGTGCCGGGA
GAAAGCAGCACCACCGTTAAACAACGTGTAATGGCTGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAGTTGAACGCCTG
GCTGGATAGTCCGGAAATACGCAAATTCTGCAAGCTTGAGAGCGAAGATGCGCAGTGGCTGGAAGAAACGCTGATCCATC
TGGGGTTATCGATTCGTGCCTGGCAGCGGTTATTGAAAGTTGCACGAACCATTGCTGATATTGATCAGTCCGACATTATC
ACACGTCAGCATTTGCAGGAGGCAGTTAGCTATCGTGCCATTGACCGTTTGCTCATCCATCTGCAAAAACTACTGACATA
A

Domains

Predicted by InterProScan.

Subunit ChlI of Mg-chelatase (ChlI)

(21-142)

Magnesium chelatase, subunit ChlI C-terminal (Mg_chelatase_C)

(400-492)

Magnesium chelatase, subunit ChlI (Mg_chelatase)

(189-386)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Haemophilus influenzae Rd KW20

58.513

100

0.591
  comM Vibrio cholerae O1 biovar El Tor strain E7946

59.163

99.209

0.587
  comM Vibrio cholerae strain A1552

59.163

99.209

0.587
  comM Glaesserella parasuis strain SC1401

58.383

100

0.585
  comM Vibrio campbellii strain DS40M4

57.968

99.209

0.575
  comM Acinetobacter baylyi ADP1

48.996

98.419

0.482
  comM Legionella pneumophila str. Paris

48.193

98.419

0.474
  comM Legionella pneumophila strain ERS1305867

48.193

98.419

0.474
  RA0C_RS07335 Riemerella anatipestifer ATCC 11845 = DSM 15868

44.269

100

0.443