Detailed information NTG0290049

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: DDT10_RS12265
• Genome accession: NZ_CP029071
• Coordinates: 2537516..2538130 (-)
• Length: 204 a.a.
• NCBI ID: WP_053573180.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain ALB79
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2532516..2543130

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DDT10_RS12240 (DDT10_12240)	rpsT	2532816..2533082 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
DDT10_RS12245 (DDT10_12245)	holA	2533099..2534140 (-)	1042	Protein_2339	DNA polymerase III subunit delta	-
DDT10_RS19685	-	2534180..2534334 (-)	155	Protein_2340	hypothetical protein	-
DDT10_RS12250 (DDT10_12250)	-	2534375..2534509 (+)	135	WP_003152870.1	YqzM family protein	-
DDT10_RS12255 (DDT10_12255)	comEC	2534528..2536879 (-)	2352	WP_053573181.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
DDT10_RS12260 (DDT10_12260)	-	2536880..2537449 (-)	570	WP_003152868.1	ComE operon protein 2	-
DDT10_RS12265 (DDT10_12265)	comEA	2537516..2538130 (-)	615	WP_053573180.1	helix-hairpin-helix domain-containing protein	Machinery gene
DDT10_RS12270 (DDT10_12270)	comER	2538189..2539010 (+)	822	WP_015417855.1	late competence protein ComER	-
DDT10_RS12275 (DDT10_12275)	-	2539079..2539816 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
DDT10_RS12280 (DDT10_12280)	rsfS	2539813..2540169 (-)	357	WP_007408260.1	ribosome silencing factor	-
DDT10_RS12285 (DDT10_12285)	yqeK	2540187..2540747 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
DDT10_RS12290 (DDT10_12290)	-	2540737..2541306 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
DDT10_RS12295 (DDT10_12295)	yhbY	2541317..2541607 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
DDT10_RS12300 (DDT10_12300)	aroE	2541601..2542443 (-)	843	WP_039254404.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22183.34 Da
• Isoelectric Point: 6.4888

>NTDB_id=290049 DDT10_RS12265 WP_053573180.1 2537516..2538130(-) (comEA) [Bacillus amyloliquefaciens strain ALB79]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTMVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=290049 DDT10_RS12265 WP_053573180.1 2537516..2538130(-) (comEA) [Bacillus amyloliquefaciens strain ALB79]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTTGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATGGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	32.328	100	0.368
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363